CD3e Antibody Alexa Fluor 700 56-0033-82 from Thermo Fisher Scientific

Product Specs
ItemCD3e Antibody Alexa Fluor 700
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number56-0033-82
Quantity100 µg
ApplicationsFlow Cytometry
Concentration0.2 mg/mL
Conjugate/TagAlexa Fluor™ 700
FormatPBS with 0.09% sodium azide; pH 7.2
ReactivityMouse
TargetCD3e
IsotypeIgG
CloneeBio500A2 (500A2)
HostSyrian Hamster
ClonalityMonoclonal
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
NCBI Full Gene NameCD3 antigen, delta polypeptide, CD247 antigen, CD3 antigen, gamma polypeptide, CD3 antigen, epsilon polypeptide
NCBI Gene AliasesCD3epsilon, Ctg-3, Cd3z, Cd3-zeta, Cd3-eta, Ctg3, T3g, T3d, Cd3, Tcrk, Cd3h, Tcrz, T3z, Cd3zeta, CD3, T3e, 4930549J05Rik, A430104F18Rik
Storage4°C, store in dark, DO NOT FREEZE!
Antibody TypePrimary

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Website: www.thermofisher.com

(1) ClpX-dependent regulatory pathway exacerbates Streptococcus pyogenes pathogenesis in diabetic skin infectionmBioJuly 8, 2026Shuiqiao Liu, Anne L. Rosen, Benjamin S. Olson, Yongke Lu, Suzanne Hickerson, Andrew L. Kau, Scott J. Hultgren, Michael G. Caparon, Wei Xu(25-0114-U025), and PE-Cy7 anti-CD11b (60-0112-U100). Alexa Fluor 700 anti-CD3e was from Invitrogen (56-0033-82). APC anti-mCD74 was from R&D Systems (FAB7478A). Validation information for these antibodies is available

(2) Host cysteine proteases promote the severity of catheter-associated urinary tract infection and kidney fibrosismBioNovember 12, 2025Wei Xu, Jian Chen, Lisa K. McLellan, Ana L. Flores-Mireles, David A. Hunstad, Michael G. CaparonAPC-Cy7 anti-CD11c (25-0114-U025), and PE-Cy7 anti-CD11b (60-0112-U100). Alexa Fluor 700 anti-CD3ε (56-0033-82) and anti-Mo CD170 (Siglec F, 414-1702-80) were from Invitrogen. Validation information for these antibodies

(3) A refined low-dose murine model of Mycobacterium ulcerans infection to assess integrated immune networks in Buruli ulcer pathogenesismBioSeptember 10, 2025Stephen Muhi, Isabelle J. H. Foo, Lukasz Kedzierski, Jessica L. Porter, Hayley A. McQuilten, Brian Howden, Katherine Kedzierska, Andrew H. Buultjens, Brendon Y. Chua, Timothy P. Stinear(A1exF5, 56-3697-82, 1:100), anti-CD206-PE (MR6F3, 12-2061-82, 1:100), and anti-CD3-AF700 (eBio500A2, 56-0033-82, 1:200). Cell viability was determined by staining with Live/Dead-Aqua 525 (L34966A, ThermoFisher, 1:800).

(4) Reprogramming aerobic metabolism mitigates Streptococcus pyogenes tissue damage in a mouse necrotizing skin infection modelNature communicationsMarch 15, 2025Xu W, Bradstreet TR, Zou Z, Hickerson S, Zhou Y, He H, Edelson BT, Caparon MG.(25-0114-U025), PE-Cy7 anti-CD11b (60-0112-U100). Alexa FluorTM 700 anti-CD3ε was from Invitrogen (56-0033-82). Validation information for these antibodies is available at vendor websites. Cells were stained for

(5) The gut microbe pair of Oribacterium sp. GMB0313 and Ruminococcus sp. GMB0270 confers complete protection against SARS-CoV-2 infection by activating CD8+ T cell-mediated immunityGut microbesJanuary 1, 2024Wang M, Lkhagva E, Kim S, Zhai C, Islam MM, Kim HJ, Hong ST.cell surface markers. PBMCs were stained with Alexa Fluor 700 anti-hamster CD3e (Thermo Fisher Lot #56003382), APC anti-hamster CD4 (Thermo Fisher Lot #17004182), FITC anti-hamster CD8a (Thermo Fisher Lot #1 1,008,182),

(6) Reprogramming Short-Chain Fatty Acid Metabolism Mitigates Tissue Damage for Streptococcus pyogenes Necrotizing Skin InfectionResearch squareDecember 23, 2023Caparon M, Xu W, Bradstreet T, Zou Z, Hickerson S, Zhou Y, He H, Edelson B.anti-CD11c (25–0114-U025), PE-Cy7 anti-CD11b (60–0112-U100). Alexa Fluor™ 700 anti-CD3ɛ was from Invitrogen (56–0033-82). Cells were stained for 20 min at 4°C, washed, and fixed in 4% paraformaldehyde (Electron Microscopy

(7) Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunityPLoS neglected tropical diseasesFebruary 1, 2022Bhattacharya P, Ismail N, Saxena A, Gannavaram S, Dey R, Oljuskin T, Akue A, Takeda K, Yu J, Karmakar S, et al.HK1.4, Biolegend, Cat: 128006), anti-mouse CD3 (Clone: eBio500A2 (500A2), Thermo Fisher Scientific, Cat: 56-0033-82), anti-mouse CD4 (Clone: RM4-5, Bio legend, Cat: 100552), anti-mouse CD44 (Clone: IM7, Thermo Fisher Scientific

(8) ILC3s control airway inflammation by limiting T cell responses to allergens and microbesCell reportsNovember 23, 2021Teng F, Tachó-Piñot R, Sung B, Farber DL, Worgall S, Hammad H, Lambrecht BN, Hepworth MR, Sonnenberg GF.

(9) Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2iScienceSeptember 24, 2021Park JG, Oladunni FS, Rohaim MA, Whittingham-Dowd J, Tollitt J, Hodges MDJ, Fathallah N, Assas MB, Alhazmi W, Almilaibary A, et al.RRID:AB_2563647" Anti-IA/IE,Biolegend,"Cat# 107651, RRID:AB_2616728" Anti-CD3,Thermofisher Scientific,"Cat# 56-0033-82, RRID:AB_837094" Anti-CD4,Thermofisher Scientific,"Cat# 25-0041-82, RRID:AB_469576" Anti-CD49b,Thermofisher Scientific

(10) HIF1α is required for NK cell metabolic adaptation during virus infectioneLifeAugust 16, 2021Victorino F, Bigley TM, Park E, Yao CH, Benoit J, Yang LP, Piersma SJ, Lauron EJ, Davidson RM, Patti GJ, et al.RRID:AB_10981505",Flow (1:200) Antibody,Anti-CD3e AF700 (Syrian hamster monoclonal),Thermo Fisher Scientific,"Cat# 56-0033-82, RRID:AB_837094",Flow (2:100) Antibody,Anti-CD19 Alexa Fluor 700 (rat monoclonal),Thermo Fisher Scientific,"Cat#

(11) Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonisteLifeMay 18, 2021Glassman CR, Su L, Majri-Morrison SS, Winkelmann H, Mo F, Li P, Pérez-Cruz M, Ho PP, Koliesnik I, Nagy N, et al.monoclonal,BioLegend,Cat#100232,(1:100) Antibody,Anti-mouse CD3ε AF700 (eBio500A2) Syrian hamster monoclonal,eBioscience,Cat#56-0033-82,(1:100) Antibody,Anti-mouse CD3ε PerCP-Cy5.5 (145–2 C11) Armenian hamster monoclonal,eBioscience,Cat#44-0031-82,(1:100) Antibody,Anti-mouse

(12) Pleiotropic consequences of metabolic stress for the major histocompatibility complex class II molecule antigen processing and presentation machineryImmunityApril 13, 2021Clement CC, Nanaware PP, Yamazaki T, Negroni MP, Ramesh K, Morozova K, Thangaswamy S, Graves A, Kim HJ, Li TW, et al.212.A1), I-Ab-CLIP-AF488 (clone 15G4(Liljedahl et al., 1998), and CD3-AF700 (clone 500A2, eBioscience #56-0033-82). Live cells were also stained with DAPI for dead cell exclusion. For determination of H2-O and H2-M

(13) High expression of CD38 and MHC class II on CD8(+) T cells during severe influenza disease reflects bystander activation and trogocytosisClinical & translational immunologyJanuary 1, 2021Jia X, Chua BY, Loh L, Koutsakos M, Kedzierski L, Olshansky M, Heath WR, Chang SY, Xu J, Wang Z, et al.FITC‐I‐Ab (#116406), Pacific Blue‐I‐Ab (#116422), FITC‐CD19 (#115506) from Biolegend, USA. AF700‐CD3 (#56003382) was purchased from Invitrogen, USA. Live/Dead‐aqua 525 was purchased from Invitrogen. Briefly, cell

(14) Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription FactorCell reportsNovember 12, 2019Shen E, Rabe H, Luo L, Wang L, Wang Q, Yin J, Yang X, Liu W, Sido JM, Nakagawa H, et al.

(15) Synergized regulation of NK cell education by NKG2A and specific Ly49 family membersNature communicationsNovember 1, 2019Zhang X, Feng J, Chen S, Yang H, Dong Z.

(16) Development of a SFTSV DNA vaccine that confers complete protection against lethal infection in ferretsNature communicationsAugust 23, 2019Kwak JE, Kim YI, Park SJ, Yu MA, Kwon HI, Eo S, Kim TS, Seok J, Choi WS, Jeong JH, et al.anti-CD44–BV650 (clone IM7, BioLegend, 103049, 1:50), and anti-CD3–Alexa Fluor 700 (clone 500A2, eBioscience, 56-0033-82, 1:100). Lethal challenge of SFTSV in ferrets Vaccinated or control ferrets were challenged intramuscularly

(17) Herpes Zoster DNA Vaccines with IL-7 and IL-33 Molecular Adjuvants Elicit Protective T Cell ImmunityImmune networkOctober 1, 2018Kim AR, Park J, Kim JH, Kwak JE, Cho Y, Lee H, Jeong M, Park SH, Shin EC.

(18) γδ T cells control humoral immune response by inducing T follicular helper cell differentiationNature communicationsAugust 8, 2018Rezende RM, Lanser AJ, Rubino S, Kuhn C, Skillin N, Moreira TG, Liu S, Gabriely G, David BA, Menezes GB, et al.

(19) Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunityWellcome Open ResearchJanuary 3, 2018Emma E. Dutton, Ana Camelo, Matthew Sleeman, Ronald Herbst, Gianluca Carlesso, Gabrielle T. Belz, David R. WitherseBioscience Armenian hamster 11-0031-85 Monoclonal AF700 (1:100) eBio500A2 0.2 eBioscience Syrian hamster 56-0033-82 Monoclonal BV510 (1:100) 104 0.1 BioLegend Mouse 109837 Monoclonal CD3 CD45.2 Concentration

(20) Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunityWellcome Open ResearchDecember 14, 2017Emma E. Dutton, Ana Camelo, Matthew Sleeman, Ronald Herbst, Gianluca Carlesso, Gabrielle T. Belz, David R. WitherseBioscience Armenian hamster 11-0031-85 Monoclonal AF700 (1:100) eBio500A2 0.2 eBioscience Syrian hamster 56-0033-82 Monoclonal BV510 (1:100) 104 0.1 BioLegend Mouse 109837 Monoclonal BV785 (1:200) 104 0.2 BioLegend Mouse 109839 Monoclonal CD49b FITC

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CD3e Antibody Alexa Fluor 700 from Thermo Fisher Scientific

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CD3e Antibody Alexa Fluor 700 from Thermo Fisher Scientific

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