Western blot was performed using Anti-WIPI2 Rabbit Polyclonal Antibody (Product # PA5-54425) and a 49 kDa band corresponding to WIPI2 was observed across cell lines tested along with an uncharacterized band (*) at ~70 kDa. Membrane enriched cell extracts (30 µg lysate) of A-431 (Lane 1), THP-1 (Lane 2), Jurkat (Lane 3), SH-SY5Y (Lane 4), Raji (Lane 5) and K-562 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).