Immunoprecipitation of Alsin was performed on HAP1 WT cell lysate. Antibody-bead conjugate was prepared by adding 2 µg of Alsin Polyclonal Antibody (Product # PA5-61254) with 30 µL of Dynabeads™ Protein A (Product # 10002D) and rocked for ~1 hour at 4 degree celcius. 500 µg of protein was incubated with the antibody-bead conjugate for ~1 hours at 4 degree celcius. Following centrifugation and multiple washes, 4% starting material (SM), 4% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody. Ponceau stained transfer of blot is shown. HC = Heavy chain. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.