BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82), Mouse IL-2 Recombinant Protein (Product # 14-8021-64), and Mouse IL-4 Recombinant Protein (Product # 14-8041-80). Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours. Following restimulation, cells were fixed and permeabilized and then stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.5 µg of Rat IgG1 K Isotype Control eFluor® 450 (Product # 48-4301-82) (left) or 0.5 µg of Anti-Mouse IL-13 eFluor® 450 (right). Viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.