Fig 2: Treatment with rTsPmy specifically increases Helios+ tTreg expansion but could not induce pTreg differentiation in mLN and cLP in colitis. (A) The percentage of CD4+Foxp3+ Tregs in cLP from rTsPmy- or PBS-treated mice. The gating strategy is shown in Supplementary Figure 2. Statistical analysis is shown on the right (n = 5). (B) The frequency of Helios+ cells in Foxp3+ T cells of cLP from rTsPmy- or PBS-treated mice. The gate strategy is shown in Supplementary Figure 3. Statistical analysis is shown on the right (n = 5). (C) The percentage of Foxp3+ (eGFP) cells in CD3+CD4+ T cells of mLN (top) and cLP (bottom) at 5 weeks after CD4+Foxp3–CD45RBhi naïve T-cell adoptive transfer into Rag1 KO mice. The gate strategy is shown in Supplementary Figure 4. Frequencies are shown on the right (n = 3). Naïve T cells were from Foxp3eGFP mice. Results are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. mLN, mesenteric lymph nodes. cLP, colonic lamina propria. See also in Supplementary Figure 5.