eBioscience™ from Invitrogen Antibodies
HELIOS Monoclonal Antibody (22F6), PE-Cyanine7
25-9883-42
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Browse Similar Products: Helios Flow Cytometry Antibodies
We are currently investigating mechanisms of T cell tolerance and Helios is a transcription factor first found to be expressed by Tregs and later on by activated CD4 T cells. This anti-Helios antibody is the only monoclonal Ab commercially available for flow cytometry and we chose the PeCY7 conjugate because we wanted a bright fluorochrome which will work with our current panel for T cells.
Flow Cytometry
Mouse splenocytes
30 mins @ +4C
anti-CD16/32
None
LSR II
Splenocytes were stained with antibodies against CD4 followed by fixation and permeabilization, followed by intracellular staining for Foxp3 and Helios. Result shown is gated on CD4+ cells. We observed that Foxp3+ CD4 T cells expressed high levels of Helios compared to Foxp3- CD4 T cells.
N/A
Good separation between positive and negative populations even after fixation and permeabilization.
Good antibody against Helios.