Rat IgG2a kappa APC Isotype Control 17-4321-81 from Thermo Fisher Scientific

Product Specs
ItemRat IgG2a kappa APC Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number17-4321-81
Quantity50 µg
ApplicationsControl, Flow Cytometry
Concentration0.2 mg/mL
Conjugate/TagAPC
FormatPBS with 0.09% sodium azide; pH 7.2
TargetRat IgG2a kappa
IsotypeIgG2a kappa
CloneeBR2a
HostRat
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypeIsotype Control
Storage4°C, store in dark, DO NOT FREEZE!
ClonalityMonoclonal

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Website: www.thermofisher.com

(1) Intact lymph node homing and CD8+ T-cell priming abilities of Sprouty2-deficient dendritic cellsFrontiers in ImmunologyMarch 18, 2026Nadine Anslinger, Guerric P. B. Samson, Vladimir Purvanov, Michael Basler, Daniel F. LeglerAPC-conjugated rat IgG2a kappa isotype control (clone eBR2a; eBioscience; Thermo Fisher Scientific; #17-4321-81), APC-conjugated anti-CD86 (clone GL-1; BioLegend; #105012), APC-conjugated anti-CD80 (clone 16-10A1;

(2) Immune Regulatory Role of Parasympathetic Nervous System in Murine Corneal Allografts via Inhibition of Dendritic Cell Activation and T Cell PolarizationInvest Ophthalmol Vis SciMarch 12, 2026Dong Ihll Lee, So Young Kim, Yong Woo Ji, Areum Yeo, Jiwon Jeon, Jong Suk Song, Hyung Keun LeePE, cat# 12-4301-82; eBioscience] for CD25 and Rat IgG2a κ isotype control [clone eBR2a, APC, cat# 17-4321-81, eBioscience] for FoxP3) were stained under identical conditions and applied specifically within the

(3) The influence of adrenoceptor blocker treatment on fracture healing in osteoporotic and non-osteoporotic boneFrontiers in PhysiologyFebruary 16, 2026Sandra Dieterich, Christoph Kölbl, Miriam Eva Angelica Tschaffon-Müller, Oliver Küppers, Dorothea Gebauer, Melanie Rebecca Kuhn, Katarina Teresa Acker, Anita Ignatius, Melanie Haffner-LuntzereBioscience), Armenian hamster IgG PE-Cyanine7 (1:100, 25-4888-82, eBioscience), rat IgG2a κ APC (1:800, 17-4321-81, eBioscience), rat IgG κ APC-eFluor 780 (1:200, 47-4031-82, eBioscience), rat IgG2a κ Alexa Fluor 700

(4) Single-cell RNA sequencing reveals disease associated changes in brain endothelial cells in the 5XFAD mouseFluids and Barriers of the CNSJanuary 30, 2026Rebecca J. Embalabala, Haley Masters, Elaina Ziehm, Jamie Pouncey, Hyosung Kim, Ethan S. Lippmanncompensation controls and isotype controls (0.1 µg/mL, Miltenyi Biotec 130-123-273; 0.1 µg/mL, Invitrogen 17-4321-81). CD31+/CD45- cells were isolated using fluorescence activated cell sorting (FACS) using a 5-laser BD

(5) A preclinical model for the identification of therapeutically active transgenes in local cancer immunotherapyOncoImmunologyDecember 31, 2025Arne Menze, Dmitrij Ostroumov, Hans Heinrich Wedemeyer, Valery Volk, Friedrich Feuerhake, Florian Kühnel, Thomas Christian Wirth(Thermo Fisher Scientific Cat#12–4888–83; RRID: AB_470074) Rat IgG2a κ Isotyp (Thermo Fisher Scientific Cat#17–4321–81; RRID: AB_470181), rat IgG2a κ isotype (Biolegend Cat#400512; RRID: AB_2814702). The following antibodies

(6) Epigenetic and transcriptional programming of murine eosinophils in the esophagusNature CommunicationsNovember 25, 2025Jennifer M. Felton, Lee E. Edsall, Ty D. Troutman, Lydia Mack, Michael Kotliar, Netali Ben-Baruch Morgenstern, Annalise M. Psaila, Mark Rochman, Andrea M. Klingler, Garrett A. Osswald, et al.Biolegend; 101229 [isotype, rat IgG2bK; 400629]), CD101 (APC, Invitrogen; 17-1011-82 [isotype, rat IgG2aK; 17-4321-81]), ST2 (BUV737, BD; 749323 [isotype, rat IgG2aK; 612760]), TLR4 (PE-Cy7, Biolegend; 145408 [isotype,

(7) Metformin-associated gut microbiota remodeling correlates with reinvigorated splenic immunity in aged mice: microbiome-immune crosstalk via the gut-spleen axisFrontiers in ImmunologySeptember 25, 2025Shu-Qin Ding, Xin-Yi Lyu, Shi-Yu Zhou, Yi-Wan Fang, Hao-Xin Ji, Jiang-Yan Li, He-Zuo Lüμg/test Invitrogen,FCM,CD163,rat monoclonal,12-1631-82,AB_2716924,PE,0.25 μg/test Invitrogen,FCM,IgG2a kappa Isotype Control,rat,17-4321-81,AB_470181,APC,0.125 μg/test Invitrogen,FCM,IgG2a kappa,rat,47-4321-82,AB_1271997,APC-eFluor™ 780,0.25

(8) NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functionsFrontiers in ImmunologySeptember 1, 2025Guerric P. B. Samson, Nadine Anslinger, Daniel F. Legler, Daniela Finke,Thermo Fisher Scientific,Cat#17-1971-82 APC-conjugated rat IgG2a kappa isotype control (CCR7 isotype; eBR2a),Thermo Fisher Scientific,Cat#17-4321-81 APC-conjugated anti-mouse CD25 (PC61.5),Thermo Fisher Scientific,Cat#17-0251-82 PE/Cy7-conjugated

(9) Human CAR Tregs Targeting SOD1 and Expressing BDNF Reduce Inflammation and Delay Disease in G93A hSOD1-NSG MiceCellsAugust 26, 2025David J. Graber, W. James Cook, Marie-Louise Sentman, Joana M. Murad-Mabaera, Elijah W. Stommel, Charles L. SentmanIntracellular staining using anti-FoxP3-APC (1/100, Invitrogen 17-4776-42) and isotype-APC (1/200, Invitrogen 17-4321-81) followed membrane permeabilization using eBioscience™ Foxp3/Transcription Factor Staining Buffer Set

(10) MET pathway inhibition increases chemo-immunotherapy efficacy in small cell lung cancerCell reports. MedicineJuly 15, 2025Del Rey-Vergara R, Galindo-Campos MA, Rocha P, Carpes M, Martínez C, Masfarré L, Menéndez S, Quimis F, Rossell A, Iñañez A, et al.Thermo Fisher Scientific), PD-L1-APC (#17-5982-82; RRID:AB_2688092; Thermo Fisher Scientific; 1:100; isotype control: #17-4321-81; RRID:AB_470181; Thermo Fisher Scientific). After staining, cells were washed once with MACS buffer

(11) Immunosuppression of spleen in mice treated with erythropoietin: transcriptomic and immunological analysisFrontiers in ImmunologyMarch 21, 2025Xinyi Lyu, Jiahao Shi, Qi Liu, Mingjun Jiang, Xilian Liu, Yulan Li, Shuqin Ding, Xianpeng Daimonoclonal,11-5698-82,AB_11151330,FITC,0.25 μg/test Invitrogen,FCM,IgG2a kappa Isotype Control,rat,17-4321-81,AB_470181,APC,0.125 μg/test Invitrogen,FCM,IgG2a kappa,rat,47-4321-82,AB_1271997,APC-eFluor™ 780,0.25

(12) Calcium Release-Activated Calcium Modulator ORAI1-Sensitive Serine Dehydratase Regulates Fatty Acid-Induced CD4+ Th17/Treg Imbalance in Dairy CowsAnimals (Basel)January 30, 2025Bingbing Zhang, Jingjing Wang, Ming Li, Jianan Wen, Juan J. Loor, Shuang Wang, Ziwei Ji, Xinquan Lv, Guihua Wang, Cheng Xia, et al.Waltham, MA, USA) antibodies were employed. Negative flow cytometry controls included Isotype Control (17-4321-81, eBioscience, Waltham, MA, USA). 2.9. Statistical Analysis Data are presented as the means ± SEM,

(13) A Novel Approach to Peripheral Nerve Regeneration: Local FK-506 Delivery Using a Reservoir Flap ModelYonsei Medical JournalNovember 12, 2024Jong Won Hong, Jung Hyun Lim, Eun Hye Kang, Young Seok KimCD25 (Cat No 554866, BD), CD45RA (Cat No 561624, BD), CD161a (Cat No 565413, BD), and FoxP3 (Cat No 17-4321-81, eBioscience). FACS preparation and analysis were conducted according to established protocols.18 Evaluation

(14) Mechanosensing regulates tissue repair program in macrophagesScience advancesMarch 15, 2024Meizlish ML, Kimura Y, Pope SD, Matta R, Kim C, Philip NH, Meyaard L, Gonzalez A, Medzhitov R.immunoglobulin 1κ (IgG1κ)–PE (clone R3-34, BD Biosciences, 554685) and rat IgG2aκ-APC (clone eBR2a, eBioscience, 17-4321-81) were also used as isotype control for gating FIZZ1- and ARG1-positive cells, respectively. After washing

(15) Human CD4+CD25+ T cells expressing a chimeric antigen receptor (CAR) against aberrant superoxide dismutase-1 (SOD1) triggers antigen-specific immunomodulationCytotherapyFebruary 1, 2024David J. Graber, W. James Cook, Marie-Louise Sentman, Joana M. Murad-Mabaera, Charles L. SentmanIntracellular staining using anti-FoxP3-APC (1/100, Invitrogen 17-4776-42) and isotype-APC (1/200, Invitrogen 17-4321-81) followed membrane permeabilization using eBioscience™ Foxp3 / Transcription Factor Staining Buffer

(16) TGF-β in the microenvironment induces a physiologically occurring immune-suppressive senescent stateCell reportsMarch 28, 2023Matsuda S, Revandkar A, Dubash TD, Ravi A, Wittner BS, Lin M, Morris R, Burr R, Guo H, Seeger K, et al.17-4801-80, Life Technologies). For flow gating, we used isotype controls of fluorescence (Cat #s: 17-4321-81, 12-4714-42 and 12-4888-81, Life Technologies). Samples were acquired on a BD Fortessa. Data were analyzed

(17) Dexmedetomidine alleviates acute lung injury by promoting Tregs differentiation via activation of AMPK/SIRT1 pathwayInflammopharmacologyFebruary 1, 2023Zhang ZT, Xie K, Luo RJ, Zhang DY, He ZW, Li KF, Lin SH, Xu F.(FJK-16 s), APC (eBioscience, 17–5773-82); Rat IgG2a kappa Isotype Control (eBR2a), APC (eBioscience, 17–4321-81). Since the antibodies used in immunohistochemistry, immunofluorescence, and western blotting experiments

(18) Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinomaFrontiers in pharmacologyJanuary 1, 2023Zhang W, Jiang X, Zou Y, Yuan L, Wang X.used as isotype control, for FOXP3-APC, Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™(17–4321-81, eBioscience) was used as isotype control, for CD25 PerCP-Cyanine5.5, Rat IgG1 kappa Isotype Control

(19) Polyethylenimine, an Autophagy-Inducing Platinum-Carbene-Based Drug Carrier with Potent Toxicity towards Glioblastoma Cancer Stem CellsCancersOctober 15, 2022McCartin C, Dussouillez C, Bernhard C, Mathieu E, Blumberger J, Dontenwill M, Herold-Mende C, Idbaih A, Lavalle P, Bellemin-Laponnaz S, et al.1/200 diluted MHC-II (#116418, Biolegend, San Diego, CA, USA) or the corresponding isotype controls (#17-4321-81, Thermo Fisher Scientific, Illkirch-Graffenstaden, France) for 30 min on ice. The cells were then washed

(20) Platelets Fuel the Inflammasome Activation of Innate Immune CellsSSRN Electronic JournalApril 1, 2022Verena Rolfes et al.Thermo Fisher Scientific 48-4301-82; RRID:AB_1271984 Rat IgG2a K Iso Control APC (eBR2a) Thermo Fisher Scientific 17-4321-81; RRID:AB_470181 Rat IgG2b, K Iso Control PE (eB149/10H5) Thermo Fisher Scientific 12-4031-82; RRID:AB_470042 2-mercaptoethanol Sigma-Aldrich M6250-250ML Acetylsalicylic acid

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Rat IgG2a kappa APC Isotype Control from Thermo Fisher Scientific

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Rat IgG2a kappa APC Isotype Control from Thermo Fisher Scientific

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