Mouse IgG2a kappa Isotype Control 14-4724-82 from Thermo Fisher Scientific

Product Specs
ItemMouse IgG2a kappa Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number14-4724-82
Quantity100 µg
ApplicationsControl, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin)
Concentration0.5 mg/mL
Conjugate/TagUnconjugated
FormatPBS with 0.09% sodium azide; pH 7.2
TargetMouse IgG2a kappa
IsotypeIgG2a kappa
CloneeBM2a
HostMouse
PurityAffinity chromatography
Storage Temperature4° C
Antibody TypeIsotype Control
Storage4°C
ClonalityMonoclonal

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Matrix structure and microenvironment dynamics correlate with chemotherapy response in ovarian canceriScienceMarch 20, 2026Florian Laforêts, Panoraia Kotantaki, Samar Elorbany, Joseph Hartlebury, Joash D. Joy, Beatrice Malacrida, Rachel C. Bryan-Ravenscroft, Chiara Berlato, Erica Di Federico, John F. Marshall, et al.RRID: AB_470111 "Mouse IgG2a kappa Isotype Control (eBM2a), eBioscience",Thermo Fisher Scientific,Cat# 14-4724-82; RRID: AB_470114 PCNA (D3H8P) XP® Rabbit mAb #13110,Cell Signaling Technology,Cat# 13110; RRID: AB_2636979 Perilipin-1

(2) Alterations in Mitochondrial DNA in Corneal Fibroblast and Myofibroblast Post InjuryInvestigative Ophthalmology & Visual ScienceJanuary 16, 2026Nishant R. Sinha, Alexandria C. Hofmann, Laila A. Suleiman, Maxwell T. Jeffrey, Rajnish Kumar, Rajiv R. Mohan(3900S; Cell Signaling Technology) was used for TFAM and Invitrogen Mouse IgG2a, kappa Isotype Control (14-4724-82; Thermo Fisher Scientific) was used for αSMA as isotype controls. RNA Extraction, cDNA Synthesis, and

(3) Marked neurotropism and potential adaptation of H5N1 clade 2.3.4.4.b virus in naturally infected domestic catsEmerging Microbes & InfectionsDecember 31, 2025Shubhada K. Chothe, Surabhi Srinivas, Sougat Misra, Noel Chandan Nallipogu, Elizabeth Gilbride, Lindsey LaBella, Swastidipa Mukherjee, Christian H. Gauthier, Heidi L. Pecoraro, Brett T. Webb, et al.was performed using Mouse IgG2a kappa Isotype Control (eBM2a), eBioscience (Thermo Fisher Catalog # 14-4724-82), which showed no cross-reaction and minimal background staining (data not shown). Fluorescence intensity

(4) Residual Breast Cancer Cells Co-opt SOX5-driven Endochondral Ossification to Maintain DormancyCancer DiscoveryDecember 11, 2025Amulya Sreekumar, Eric Blankemeyer, Christopher J. Sterner, Tien-Chi Pan, Dhruv K. Pant, Sarah Acolatse, Hamza Turkistani, George K. Belka, Anupma Nayak, Sean D. Carlin, et al.Scientific, Cat. #02–6102, RRID:AB_2532938), and Mouse IgG2a kappa (Thermo Fisher Scientific, Cat. #14–4724-82, RRID:AB_470114). Following primary antibody incubation, samples were washed three times in 1× PBS and

(5) Corrigendum to SLAMF7 and SLAMF8 receptors shape human plasmacytoid dendritic cell responses to intracellular bacteriaJournal of Clinical InvestigationSeptember 2, 2025Joaquín Miguel Pellegrini, Anne Keriel, Laurent Gorvel, Sean Hanniffy, Vilma Arce-Gorvel, Mile Bosilkovski, Javier Solera, Stéphane Méresse, Sylvie Mémet, Jean-Pierre Gorvelcatalog SAB1401970) was from Sigma-Aldrich and the mouse IgG2a κ isotype antibody (clone eBM2a, catalog 14-4724-82) for the fluorescence minus one control (FMO) from eBioscience/Thermo Fisher Scientific. EAT-2 and phospho-flow

(6) Minimally invasive delivery of engineered heart tissues restores cardiac function in rats with chronic myocardial infarctionActa BiomaterialiaAugust 30, 2025Pengsheng Li, S. M. Shatil Shahriar, Ling Tang, Hannah Prachyl, Hari Chaliki, Luis Scott, Jingwei Xie, Wuqiang ZhuD9542-10MG). Isotype-matched normal mouse IgG1 (Cell signaling, Catalog# 5415S), IgG2a (Thermo Fisher Scientific, Catalog# 14472482), IgG2b (Cell signaling, Catalog# 53484), rabbit IgG (Cell signaling, Catalog# 3900S), goat IgG (Thermo Fisher Scientific

(7) IL1RAP Expression in Human Atherosclerosis: A Target of Novel Antibodies to Reduce Vascular Inflammation and AdhesionJournal of the American Heart AssociationMay 20, 2025Madelene Lindkvist, Anna Göthlin Eremo, Geena Varghese Paramel, Sheikh Anisul Haque, Camilla Rydberg Millrud, Sara Rattik, Caitríona Grönberg, David Liberg, Allan Sirsjö, Karin Fransénanti‐IL1RAP (Cantargia AB) or a corresponding mouse IgG2a κ isotype control antibody (eBM2a, catalog number 14‐4724‐82; eBioscience/Invitrogen/Thermo Fisher Scientific) for 1 hour, followed by treatment with 5 ng/mL mature

(8) Residual Breast Cancer Cells Co-opt SOX5-driven Endochondral Ossification to Maintain DormancybioRxivMay 14, 2025Amulya Sreekumar, Eric Blankemeyer, Christopher J. Sterner, Tien-Chi Pan, Dhruv K. Pant, Sarah Acolatse, Hamza Turkistani, George K. Belka, Sean D. Carlin, Charles A. Assenmacher, et al.IgG (Thermo Fisher Scientific, Cat. #02-6102), and Mouse IgG2a kappa (Thermo Fisher Scientific, Cat. #14-4724-82). Following primary antibody incubation, samples were washed three times in 1× PBS and incubated for

(9) Generation and characterization of chicken monocyte-derived dendritic cellsFrontiers in immunologyJanuary 1, 2025Ngantcha Tatchou E, Milcamps R, Oldenhove G, Lambrecht B, Ingrao F.P3.6.2.8.1),IgG1,UNLB,14-4714-82,eBioscience,5 µg/mL Mouse IgG2a isotype control (Clone eBM2a),IgG2a,UNLB,14-4724-82,eBioscience,5 µg/mL Goat anti-mouse IgG1 (Polyclonal),Goat IgG,FITC,1070-02,Southern Biotech,10 µg/mL Goat

(10) A novel multiplexed immunoassay for surface-exposed proteins in plasma extracellular vesiclesJournal of extracellular vesiclesNovember 1, 2024Tordoff E, Allen J, Elgart K, Elsherbini A, Kalia V, Wu H, Eren E, Kapogiannis D, Gololobova O, Witwer K, et al.rat",Neuronal CD68,BioLegend,Capture,333802,Rat Monoclonal,"Mouse, human",Microglia IgG2A,Thermo Fisher Scientific,Capture control,14472482,Mouse,N/A,Isotype control Capture and detection antibodies used in LuminEV assay.

(11) Virus-like particles as robust tools for functional assessment: Deciphering the pathogenicity of ABCA4 genetic variants of uncertain significanceJournal of Biological ChemistryOctober 1, 2024Senem Cevik, Subhasis B. Biswas, Arit Ghosh, Esther E. Biswas-Fiss(rabbit IgG isotype control, Invitrogen (Cat. # 02-610-2) and mouse IgG2a kappa isotype control, eBioscience (Cat. # 14-4724-82)) for 30 min at room temperature. Subsequently, the cells were washed three times with buffer A, followed

(12) Characterization of viroplasm-like structures by co-expression of NSP5 and NSP2 across rotavirus species A to JJournal of VirologySeptember 17, 2024Melissa Lee, Ariana Cosic, Kurt Tobler, Claudio Aguilar, Cornel Fraefel, Catherine Eichwaldanti-HA (clone HA-7; Merck, H3663), or 2 µg mouse IgG2a kappa isotype control (clone eBM2a; eBioscience, 14–4724-82) and incubated at 4°C for 30 min with rotation. The cell lysates were combined with 50 µL of Protein

(13) Inflammation primes the murine kidney for recovery by activating AZIN1 adenosine-to-inosine editingJournal of Clinical InvestigationSeptember 3, 2024Segewkal Hawaze Heruye, Jered Myslinski, Chao Zeng, Amy Zollman, Shinichi Makino, Azuma Nanamatsu, Quoseena Mir, Sarath Chandra Janga, Emma H. Doud, Michael T. Eadon, et al.concentration of 10 μg per 600 μL of lysate for 2 hours at 4°C. Mouse IgG2a κ (clone eBM2a; eBioscience 14-4724-82) was used as an isotype control. Protein G Dynabeads (Invitrogen 10003D) were washed in the immunoprecipitation

(14) Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brainCellAugust 8, 2024Nicole Scott-Hewitt, Matthew Mahoney, Youtong Huang, Nils Korte, T. Yvanka Yvanka de Soysa, Daniel K. Wilton, Emily Knorr, Kevin Mastro, Allison Chang, Allison Zhang, et al.(Mouse monoclonal, clone 12D10; EMD Millipore #MABE343) or isotype control (Mouse IgG2a, ThermoFisher #14–4724-82) antibodies were added to protein lysate at a concentration of 2μg/mg of protein. Samples were processed

(15) Mechanistic insights into SARS-CoV-2 spike protein induction of the chemokine CXCL10Scientific reportsMay 16, 2024Ghazanfari D, Courreges MC, Belinski LE, Hogrell MJ, Lloyd J, C Bergmeier S, McCall KD, Goetz DJ.monoclonal antibody (mAb) (14–9922-82, ThermoFisher) or 20 µg/mL mouse IgG2a kappa isotype control (14–4724-82, ThermoFisher) for 40 min in the dark on ice. Subsequently, the treated macrophages were washed twice

(16) Distinct functions for the paralogous RBM41 and U11/U12-65K proteins in the minor spliceosomeNucleic acids researchApril 24, 2024Norppa AJ, Chowdhury I, van Rooijen LE, Ravantti JJ, Snel B, Varjosalo M, Frilander MJ.Anti-Rabbit IgG HRP (Amersham, NA934V, WB: 1:10 000). Mouse IgG2a kappa Isotype Control (Invitrogen, 14-4724-82) served as a negative control antibody in IP experiments. eBiocience Avidin-HRP (Invitrogen, 18-4100,

(17) Systems-based identification of the Hippo pathway for promoting fibrotic mesenchymal differentiation in systemic sclerosisNature CommunicationsJanuary 3, 2024Feiyang Ma, Pei-Suen Tsou, Mehrnaz Gharaee-Kermani, Olesya Plazyo, Xianying Xing, Joseph Kirma, Rachael Wasikowski, Grace A. Hile, Paul W. Harms, Yanyun Jiang, et al.isotype control antibodies: rabbit IgG (ab172730), mouse IgG1 (ab 280974) both from Abcam, IgG2ak (14-4724-82) from Invitrogen (Supplementary Data 7), antibodies were stained in parallel with each set of the slides

(18) B3GALT6 promotes dormant breast cancer cell survival and recurrence by enabling heparan sulfate-mediated FGF signalingCancer CellJanuary 1, 2024Amulya Sreekumar, Michelle Lu, Biswa Choudhury, Tien-chi Pan, Dhruv K. Pant, Matthew R. Lawrence-Paul, Christopher J. Sterner, George K. Belka, Takashi Toriumi, Brian A. Benz, et al.IgG (Thermo Fisher Scientific, Cat. # 02–6102), Mouse IgG2a kappa (Thermo Fisher Scientific, Cat. # 14-4724-82). After performing 3 washes with 1x PBS, samples were incubated with secondary antibodies at 37°C for

(19) Surgical and intravital microscopy protocol to image Trypanosoma brucei-host interactions in live rodent modelsSTAR protocolsJune 17, 2022De Niz M, Figueiredo LM.IgG2 K isotype control (eBM2a) (20 μg/ 30 g body weight),"eBioscience, Thermo Fisher Scientific",Cat# 14-4724-82 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, AlexaFluor

(20) Interferon-gamma inhibits influenza A virus cellular attachment by reducing sialic acid cluster sizeiScienceApril 15, 2022Carol Ho-Yan Fong, Lu Lu, Lin-Lei Chen, Man-Lung Yeung, Anna Jinxia Zhang, Hanjun Zhao, Kwok-Yung Yuen, Kelvin Kai-Wang To31235;RRID:AB_243593 Mouse IgG2a kappa Isotype Control (eBM2a),"Thermofisher Scientific, MA, USA",Cat# 14-4724-82; RRID: AB_470114 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, A/Hong

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Mouse IgG2a kappa Isotype Control

Mouse IgG2a kappa Isotype Control from Thermo Fisher Scientific

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Mouse IgG2a kappa Isotype Control from Thermo Fisher Scientific

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