Fig 1: Analysis of B cell Subpopulations By using scRNA-seq And Flow Cytometry. (A) A UMAP plot showing the subpopulations of B cells after performing integration of control kidney and ccRCC B cells. (B) Gene nebula maps showing the distribution of the three featured genes (MARCH3, B2M and DTWD1) in integrated B cells. (C) Violin plots showing the expression of TGF-ß1 and IL-10 in B cell subpopulations. (D) A heatmap showing the heterogeneity of the top 10 genes expressed among B cell subpopulations. (E) Stacked bar plots showing the comparison of B cell subpopulations in control kidneys and ccRCC biopsies. (F) Flow cytometry analysis of B cell subpopulations in control kidneys and ccRCC biopsies. (G) Scatter plots showing the statistical analysis of B cell subpopulations in control kidneys and ccRCC biopsies. Each dot represents one readout. The data represent 2 independent experiments. **p < 0.01; NS, not significant.