Western blot analysis was performed on Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), Caco-2 (Lane 2), A549 (Lane 3), HeLa (Lane 4), NIH/3T3 (Lane 5), NTERA-2 (Lane 6) and PC-3 (Lane 7). The blots were probed with Anti-Fatty Acid Synthase Recombinant Rabbit Monoclonal Antibody (Product # 702060, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 273 kDa band corresponding to Fatty Acid Synthase was observed across the cell ines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with wet transfer method. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).