MHC Class II (I-A/I-E) Antibody APC-eFluor 780 47-5321-82 from Thermo Fisher Scientific

Product Specs
ItemMHC Class II (I-A/I-E) Antibody APC-eFluor 780
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number47-5321-82
Quantity100 µg
ApplicationsFlow Cytometry
Concentration0.2 mg/mL
Conjugate/TagAPC-eFluor™ 780
FormatPBS with 0.09% sodium azide; pH 7.2
ReactivityMouse
TargetMHC Class II (I-A/I-E)
IsotypeIgG2b kappa
CloneM5/114.15.2
HostRat
NCBI Full Gene Namehistocompatibility 2, class II antigen A, beta 1, histocompatibility 2, class II antigen E beta
NCBI Gene AliasesH-2Eb, Rmcs1, Ia2, I-Abeta, IAb, Eb, H-2Ab, Abeta, H2Eb, Ia-4, H2-Ab, Ia4, Ia-2
ClonalityMonoclonal
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypePrimary
Storage4° C, store in dark, DO NOT FREEZE!

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Intracavitary adoptive transfer of IL-12 mRNA-engineered tumor-specific CD8+ T cells eradicates peritoneal metastases in mouse modelsOncoImmunologyDecember 31, 2023Claudia Augusta Di Trani, Assunta Cirella, Leire Arrizabalaga, Ángela Bella, Myriam Fernandez-Sendin, Joan Salvador Russo-Cabrera, Celia Gomar, Irene Olivera, Elizabeth Bolaños, José González-Gomariz, et al.134018, PE-CD45.1 #110743, FITC-Ly6C # 128006, APC-PD-L1 # 124311, APC-eFluor780-MHCII (I-A/I-E) # 47–5321-82 eBioscience (San Diego, CA, USA), PE-Dazzle594-CD38 # 102729, BV421-F4/80 # 123131, BV510-LY6G # 127633,

(2) Intratumoral injection of IL-12-encoding mRNA targeted to CSF1R and PD-L1 exerts potent anti-tumor effects without substantial systemic exposureMolecular Therapy - Nucleic AcidsSeptember 12, 2023Claudia Augusta Di Trani, Assunta Cirella, Leire Arrizabalaga, Maite Alvarez, Ángela Bella, Myriam Fernandez-Sendin, Joan Salvador Russo-Cabrera, Celia Gomar, Nuria Ardaiz, Alvaro Teijeira, et al.APC/Fire750-Tim-3 (#134018); FITC-Ly6C (#128006); PerCP5.5-CD45.2 (#109828); APC-eFluor780-MHC-II (I-A/I-E) (#47-5321-82; eBioscience); PE-Dazzle594-CD38 (#102729); BV421-F4/80 (#123131); BV510-LY6G (#127633); BV785-TCRb

(3) Localized immune surveillance of primary melanoma in the skin deciphered through executable modelingScience AdvancesApril 14, 2023Rowan Howell, James Davies, Matthew A. Clarke, Anna Appios, Inês Mesquita, Yashoda Jayal, Ben Ringham-Terry, Isabel Boned Del Rio, Jasmin Fisher, Clare L. Bennett(eBioscience, no. 48-0112-82, RRID AB_1582236), anti–MHCII-APC-Cy7/780 (clone M5/114, eBioscience, no. 47-5321-82, RRID AB_1548783), anti-CD24-BV650 (clone M1/69, BD Horizon, no. 563545, RRID AB_2738271), and anti–EpCAM-APC

(4) NLRP12-expressing dendritic cells mediate both dissemination of infection and adaptive immune responses in visceral leishmaniasisiScienceMarch 17, 2023Diogo Garcia Valadares, Owen Scott Clay, Yani Chen, Breanna Mary Scorza, Suzanne Louise Cassel, Fayyaz Shiraz Sutterwala, Mary Edythe Wilson17-5931-82; RRID:AB_469476 Monoclonal anti-MHCII (I-A/E)-PE-Cy.7 (clone MS/114.15.2),eBioscience,Cat# 47-5321-82; RRID:AB_1548783 Monoclonal anti-CD45.2-FITC (clone 104),eBioscience,Cat# 11-0454-82; RRID:AB_465061 Monoclonal

(5) LAMP2 regulates autophagy in the thymic epithelium and thymic stroma-dependent CD4 T cell developmentAutophagyFebruary 1, 2023Rodrigues PM, Sousa LG, Perrod C, Maceiras AR, Ferreirinha P, Pombinho R, Romera-Cárdenas G, Gomez-Lazaro M, Senkara M, Pistolic J, et al.(In1/CD74, Biolegend, 151,003); APC-eFluor780-conjugated anti-I-A/I-E (clone M5/114-15-2; eBioscience, 47–5321-82); eFluor450-conjugated anti-CD24 (clone M1/69; eBioscience, 48–0242-82); BV421 conjugated anti-EPCAM

(6) Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentationCell reportsAugust 16, 2022Gros M, Segura E, Rookhuizen DC, Baudon B, Heurtebise-Chrétien S, Burgdorf N, Maurin M, Kapp EA, Simpson RJ, Kozik P, et al.(clone B20.1, eBioscience #12-5812-82), anti-MHC-II-APC eFluor780 (clone M5/114.15.2, eBioscience #47-5321-82), anti-MHC-II-eFluor450 (clone M5/114.15.2, eBioscience #48-5321-82), anti-MHC-II-PerCPCy5.5 (clone

(7) Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platformGenes & developmentAugust 1, 2022Naranjo S, Cabana CM, LaFave LM, Romero R, Shanahan SL, Bhutkar A, Westcott PMK, Schenkel JM, Ghosh A, Liao LZ, et al.BD Biosciences 559864), EpCAM-PE (1:500; Biolegend 118206), and MHCII-APC-eFluor-780 (1:500; Thermo 47-5321-82) for 30 min on ice; and then resuspended in FACS buffer containing 1 µg/mL DAPI (Thermo D1306). The

(8) Cancer cells dying from ferroptosis impede dendritic cell-mediated anti-tumor immunityNature communicationsJune 27, 2022Wiernicki B, Maschalidi S, Pinney J, Adjemian S, Vanden Berghe T, Ravichandran KS, Vandenabeele P.buffer and stained for surface markers: CD11c-BV650 (#117339, BioLegend, 1:200), MHCII-APC/eFluor700 (#47-5321-82, eBioscience, 1:100), CD86-PE/Cy7 (#105116, BioLegend, 1:200), CD80-PE/Cy5 (#104712, BioLegend, 1:200),

(9) Neutrophil-dendritic cell interaction plays an important role in live attenuated Leishmania vaccine induced immunityPLoS neglected tropical diseasesFebruary 1, 2022Bhattacharya P, Ismail N, Saxena A, Gannavaram S, Dey R, Oljuskin T, Akue A, Takeda K, Yu J, Karmakar S, et al.16-10A1, BD), anti-mouse MHCII Class II (I-A/I-E) (Clone: M5/114.15.2, Thermo Fisher Scientific, Cat: 47-5321-82), anti-mouse CD40 (Clone: 1C10, Thermo Fisher Scientific, Cat: 17-0401-82), anti-mouse CD205 (Clone:

(10) Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2Cell death discoveryOctober 29, 2021Zhou N, Liu W, Zhang W, Liu Y, Li X, Wang Y, Zheng R, Zhang Y.

(11) Adenosine-to-inosine editing of endogenous Z-form RNA by the deaminase ADAR1 prevents spontaneous MAVS-dependent type I interferon responsesImmunitySeptember 14, 2021Tang Q, Rigby RE, Young GR, Hvidt AK, Davis T, Tan TK, Bridgeman A, Townsend AR, Kassiotis G, Rehwinkel J.X54-5/7.1),Biolegend,Cat# 139304; RRID: AB_10612740 Anti-MHCII-e780 (Clone M5/114.15.2),eBioscience,Cat# 47-5321-82; RRID: AB_1548783 Anti-CD31-PEcy7 (Clone 390),eBioscience,Cat# 25-0311-81; RRID: AB_2734962 Anti-CD326-APC

(12) CXCL10(+) peripheral activation niches couple preferred sites of Th1 entry with optimal APC encounterCell reportsAugust 10, 2021Prizant H, Patil N, Negatu S, Bala N, McGurk A, Leddon SA, Hughson A, McRae TD, Gao YR, Livingstone AM, et al.20/70),Biolegend,Cat# 135808; RRID: AB_10899415 anti-mouse MHC-II APC-ef780 (Clone M5/114.14.2),eBioscience,Cat# 47-5321-82; RRID: AB_1548783 anti-mouse IFNg APC (clone XMG1.2),Ebioscience,Cat# 17-7311-82; RRID: AB_469504 anti-mouse

(13) Synergistic immunotherapy of glioblastoma by dual targeting of IL-6 and CD40Nature communicationsJune 8, 2021Yang F, He Z, Duan H, Zhang D, Li J, Yang H, Dorsey JF, Zou W, Nabavizadeh SA, Bagley SJ, et al.

(14) Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast CancerBio-protocolJune 5, 2021Almeida AS, Fein MR, Egeblad M.

(15) Topical arginase inhibition decreases growth of cutaneous squamous cell carcinomaScientific reportsMay 24, 2021Mittal A, Wang M, Vidyarthi A, Yanez D, Pizzurro G, Thakral D, Tracy E, Colegio OR.

(16) CD8(+) T cell self-tolerance permits responsiveness but limits tissue damageeLifeApril 30, 2021Truckenbrod EN, Burrack KS, Knutson TP, Borges da Silva H, Block KE, O'Flanagan SD, Stagliano KR, Hurwitz AA, Fulton RB, Renkema KR, et al.RRID:AB_2621857,Clone 30-H12 Antibody,Anti-mouse MHC Class II (I-A/I-E) APC ef780,Thermo Fisher Scientific,Cat. #: 47-5321-82; RRID:AB_1548783,Clones M5/114.15.2 Antibody,Anti-mouse MHC Class II (I-A/I-E) BV510,BioLegend,Cat.

(17) Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of MelanomaCell reportsJanuary 12, 2021Budhu S, Giese R, Gupta A, Fitzgerald K, Zappasodi R, Schad S, Hirschhorn D, Campesato LF, De Henau O, Gigoux M, et al.

(18) Inhibition of the Inflammasome Activity of NLRP3 Attenuates HDM-Induced Allergic AsthmaFrontiers in immunologyJanuary 1, 2021Ma M, Li G, Qi M, Jiang W, Zhou R.(Biolegend, 115520, 6D5), SiglecF-Alexa Fluor 647 (BD, 562680, E50-2440), MHCII-APC-eFluor 780 (eBioscience, 47-5321-82, M5/114.15.2), Ly-6G-BV421 (Biolegend, 127628, 1A8), CD45-BV510 (Biolegend, 103137, 30-F11), CD11c-BV510

(19) Antagonistic Inflammatory Phenotypes Dictate Tumor Fate and Response to Immune Checkpoint BlockadeImmunityDecember 15, 2020Bonavita E, Bromley CP, Jonsson G, Pelly VS, Sahoo S, Walwyn-Brown K, Mensurado S, Moeini A, Flanagan E, Bell CR, et al.RRID: AB_493727 anti-MHCII I-A/I-E- APC- eFluor780 (Clone M5/114.15.2),Thermo Fisher Scientific,Cat# 47-5321-82; RRID: AB_1548783 anti-CD11c-PerCP/Cy5.5 (Clone N418),BioLegend,Cat# 117328; RRID: AB_2129641 anti-

(20) Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosisJCI insightDecember 3, 2020Lu S, Jolly AJ, Strand KA, Dubner AM, Mutryn MF, Moulton KS, Nemenoff RA, Majesky MW, Weiser-Evans MC.

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9/11/2017MHC Class II (I-A/I-E) Monoclonal Antibody For Flow Cytometry Nupur BhattacharyaI am interested in characterizing antigen presenting cell populations in the murine colonic lamina propria in the context of colon cancer. Therefore, we optimized the stain for MHCII.

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MHC Class II (I-A/I-E) Antibody APC-eFluor 780 from Thermo Fisher Scientific

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MHC Class II (I-A/I-E) Antibody APC-eFluor 780 from Thermo Fisher Scientific

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