MHC Class II (I-A/I-E) Monoclonal Antibody For Flow Cytometry

September 11, 2017

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), APC-eFluor 780, eBioscience™

Catalog Number:

47-5321-82

I am interested in characterizing antigen presenting cell populations in the murine colonic lamina propria in the context of colon cancer. Therefore, we optimized the stain for MHCII.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse colon tissue sample

Primary Incubation

1:200 dilution; 20 min at 4 deg C

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

The MHCII positive population is clearly seen

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Staining is clear

The Bad

None

The Bottom Line

This is a good antibody

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