CD69 Antibody PerCP-eFluor 710 46-0699-42 from Thermo Fisher Scientific

Product Specs
ItemCD69 Antibody PerCP-eFluor 710
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number46-0699-42
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypePrimary
Storage4°C, store in dark, DO NOT FREEZE!
ReactivityHuman (Hu)
ClonalityMonoclonal
NCBI Gene AliasesAIM, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3
NCBI Full Gene NameCD69 molecule
ApplicationsFlow Cytometry (FCM)
HostMouse
CloneFN50
IsotypeIgG1 kappa
TargetCD69
FormatPBS with BSA and 0.09% sodium azide; pH 7.2
Conjugate/TagPerCP-eFluor™ 710
Concentration5 µL/Test
Quantity100 Tests

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Enhancing CAR T‐cell therapy manufacturing efficiency through semi‐automated bioprocessingClin Transl ImmunologyJune 2, 2025Jason Isaacson, Prajakta Bhanap, Nicholas Putnam, Jasmine Padilla, Nujhat Fatima, Max Dotson, Danny Hayoun, Moloud Ahmadi, Gertrude Nonterah, Yongchang Ji(47‐0036‐42) CD4,Invitrogen (67‐0049‐42) CD8,Invitrogen (56‐0087‐42) CD25,Invitrogen (61‐0257‐42) CD69,Invitrogen (46‐0699‐42) HLA‐DR,Invitrogen (63‐9956‐42) CD2,Invitrogen (78‐0029‐42) SYTOX Blue dead cell stain,Invitrogen

(2) DNAJB1-PRKACA fusion neoantigens elicit rare endogenous T cell responses that potentiate cell therapy for fibrolamellar carcinomaCell reports. MedicineMarch 19, 2024Kirk AM, Crawford JC, Chou CH, Guy C, Pandey K, Kozlik T, Shah RK, Chung S, Nguyen P, Zhang X, et al.605-conjugated, Biolegend 502936, clone MAb11), and 1 μL anti-human CD69 (PerCP/efluor710-conjugated, eBioscience 46-0699-42, clone FN50). For TCR-transduced primary T cells, this cocktail included 1.25 μL anti-human IFNγ (Alexa

(3) Selection, engineering, and in vivo testing of a human leukocyte antigen-independent T-cell receptor recognizing human mesothelinPloS oneJanuary 1, 2024Hiscox MJ, Wasmuth A, Williams CL, Foot JN, Wiedermann GE, Fadda V, Boiani S, Cornforth TV, Wikiert KA, Bruton S, et al.

(4) Comprehensive Single-Cell Immune Profiling Defines the Patient Multiple Myeloma Microenvironment Following Oncolytic Virus Therapy in a Phase Ib TrialClinical cancer research : an official journal of the American Association for Cancer ResearchDecember 15, 2023Nawrocki ST, Olea J, Villa Celi C, Dadrastoussi H, Wu K, Tsao-Wei D, Colombo A, Coffey M, Fernandez Hernandez E, Chen X, et al.

(5) Spontaneous HIV expression during suppressive ART is associated with the magnitude and function of HIV-specific CD4+ and CD8+ T cellsCell Host & MicrobeSeptember 13, 2023Mathieu Dubé, Olivier Tastet, Caroline Dufour, Gérémy Sannier, Nathalie Brassard, Gloria-Gabrielle Delgado, Amélie Pagliuzza, Corentin Richard, Manon Nayrac, Jean-Pierre Routy, et al.Lot:8288818; RRID:AB_2744432 FN50 (PerCP-eFluor710) [Human anti-CD69],Thermo Fisher Scientific,Cat#46-0699-42; Lot:1920361; RRID:AB_2573694 FN50 (BV650) [Human anti-CD69],Biolegend,Cat# 310934; Lot:B303462; RRID:AB_2563158 H4A3

(6) A third SARS-CoV-2 mRNA vaccine dose in people receiving hemodialysis overcomes B cell defects but elicits a skewed CD4(+) T cell profileCell reports. MedicineMarch 21, 2023Sannier G, Nicolas A, Dubé M, Marchitto L, Nayrac M, Tastet O, Chatterjee D, Tauzin A, Lima-Barbosa R, Laporte M, et al.,BD Biosciences,"Cat#564448;Lot:8,288,818;RRID:AB_2744432" FN50 (PerCP-eFluor710) [Human anti-CD69],Thermo Fisher Scientific,"Cat#46-0699-42;Lot:1,920,361;RRID:AB_2573694" FN50 (BV650) [Human anti-CD69],Biolegend,Cat# 310934;Lot:B303462;RRID:AB_2563158 H4A3

(7) An extended SARS-CoV-2 mRNA vaccine prime-boost interval enhances B cell immunity with limited impact on T cellsiScienceJanuary 20, 2023Nicolas A, Sannier G, Dubé M, Nayrac M, Tauzin A, Painter MM, Goel RR, Laporte M, Gendron-Lepage G, Medjahed H, et al.Lot:8288818; RRID:AB_2744432 FN50 (PerCP-eFluor710) [Human anti-CD69],Thermo Fisher Scientific,Cat#46-0699-42; Lot:1920361; RRID:AB_2573694 FN50 (BV650) [Human anti-CD69],Biolegend,Cat# 310934; Lot:B303462; RRID:AB_2563158 H4A3

(8) Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infectionFASEB journal : official publication of the Federation of American Societies for Experimental BiologySeptember 1, 2022Ding C, Hong Y, Che Y, He T, Wang Y, Zhang S, Wu J, Xu W, Hou J, Hao H, et al.

(9) SARS CoV-2 mRNA vaccination exposes latent HIV to Nef-specific CD8(+) T-cellsNature communicationsAugust 19, 2022Stevenson EM, Terry S, Copertino D, Leyre L, Danesh A, Weiler J, Ward AR, Khadka P, McNeil E, Bernard K, et al.

(10) Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimenCell reportsJune 28, 2022Nayrac M, Dubé M, Sannier G, Nicolas A, Marchitto L, Tastet O, Tauzin A, Brassard N, Lima-Barbosa R, Beaudoin-Bussières G, et al.Lot:8288818 ; RRID:AB_2744432 FN50 (PerCP-eFluor710) [Human anti-CD69],Thermo Fisher Scientific,Cat#46-0699-42 ; Lot:1920361 ; RRID:AB_2573694 FN50 (BV650) [Human anti-CD69],Biolegend,Cat# 310934 ; Lot:B303462

(11) SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8(+) T cellsNature immunologyMay 1, 2022Minervina AA, Pogorelyy MV, Kirk AM, Crawford JC, Allen EK, Chou CH, Mettelman RC, Allison KJ, Lin CY, Brice DC, et al.647-conjugated, Biolegend 502516, clone 4S.B3) and 1 μL anti-human CD69 (PerCP-eFluor710-conjugated, eBioscience 46-0699-42, clone FN50) at 4 °C for 30 minutes. Cells were washed twice with 1X Perm/Wash buffer, and then were

(12) SARS-CoV-2 mRNA vaccination elicits a robust and persistent T follicular helper cell response in humansCellFebruary 17, 2022Mudd PA, Minervina AA, Pogorelyy MV, Turner JS, Kim W, Kalaidina E, Petersen J, Schmitz AJ, Lei T, Haile A, et al.AB_2561668 CD69 PerCP-eFluor 710 (clone FN50),CD69 PerCP-eFluor 710 (clone FN50),eBioscience,cat#: 460699-42; RRID: AB_2573694 CXCR5 Super Bright 436 (clone MU5UBEE),CXCR5 Super Bright 436 (clone MU5UBEE),eBioscience,cat#:

(13) SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8 T cellsmedRxiv : the preprint server for health sciencesJanuary 26, 2022Minervina AA, Pogorelyy MV, Kirk AM, Crawford JC, Allen EK, Chou CH, Mettelman RC, Allison KJ, Lin CY, Brice DC, et al.647-conjugated, Biolegend 502516, clone 4S.B3) and 1 μL anti-human CD69 (PerCP-eFluor710-conjugated, eBioscience 46-0699-42, clone FN50) at 4 °C for 30 minutes. Cells were washed twice with 1X Perm/Wash buffer, and then were

(14) Impaired T helper cell responses in human immunodeficiency virus-exposed uninfected newbornsImmunity, inflammation and diseaseDecember 1, 2021Brito-Pérez Y, Camacho-Pacheco RT, Plazola-Camacho N, Soriano-Becerril D, Coronado-Zarco IA, Arreola-Ramírez G, González-Pérez G, Herrera-Salazar A, Flores-González J, Bermejo-Haro MY, et al.Beckman Coulter), ‐CD56‐BV421 (Clone HCD56, Cat. 318328; Biolegend), ‐CD69‐PERCPef710 (Clone FN50, Cat. 46‐0699‐42; eBioscience™), ‐CD279‐PE (Clone EH12.1, Cat. 560795; BD Biosciences), ‐CD45‐PECy7 (Clone HI30, Cat.

(15) A single dose of the SARS-CoV-2 vaccine BNT162b2 elicits Fc-mediated antibody effector functions and T cell responsesCell host & microbeJuly 14, 2021Tauzin A, Nayrac M, Benlarbi M, Gong SY, Gasser R, Beaudoin-Bussières G, Brassard N, Laumaea A, Vézina D, Prévost J, et al.Lot:8288818; RRID:AB_2744432 FN50 (PerCP-eFluor710) [Human anti-CD69],Thermo Fisher Scientific,Cat#46-0699-42; Lot:1920361; RRID:AB_2573694 FN50 (BV650) [Human anti-CD69],Biolegend,Cat# 310934; Lot:B303462; RRID:AB_2563158 H4A3

(16) Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphomaNature communicationsMay 17, 2021Zhang S, Jiang VC, Han G, Hao D, Lian J, Liu Y, Zhang R, McIntosh J, Wang R, Dang M, et al.

(17) Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vesselsMolecular therapy : the journal of the American Society of Gene TherapyApril 7, 2021Zhou C, Wei W, Ma J, Yang Y, Liang L, Zhang Y, Wang Z, Chen X, Huang L, Wang W, et al.

(18) Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironmentCell death and differentiationFebruary 1, 2021Zhou C, Zhang Y, Yan R, Huang L, Mellor AL, Yang Y, Chen X, Wei W, Wu X, Yu L, et al.

(19) Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivoTheranosticsJanuary 1, 2021Zhong L, Shi W, Gan L, Liu X, Huo Y, Wu P, Zhang Z, Wu T, Peng H, Huang Y, et al.

(20) Identification of celastrol as a novel HIV-1 latency reversal agent by an image-based screenPloS oneJanuary 1, 2021Liu H, Hu PW, Dubrulle J, Stossi F, Nikolai BC, Mancini MA, Rice AP.

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CD69 Antibody PerCP-eFluor 710

CD69 Antibody PerCP-eFluor 710 from Thermo Fisher Scientific

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CD69 Antibody PerCP-eFluor 710 from Thermo Fisher Scientific

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