Western blot was performed using LDB1 Rabbit Polyclonal Antibody (Product # PA5-56948) and 46kDa, 42kDa bands corresponding to LDB1 was observed across cell lines along with an uncharacterized band (*) at ~60kDa in the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of PANC-1 (Lane 1), A-431 (Lane 2), HeLa (Lane 3), Hep G2 (Lane 4) and MCF7 (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (0.4ug/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).