Anti-PCNA antibody [EPR3821] ab92552 from Abcam

Product Specs
ItemAnti-PCNA antibody [EPR3821]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab92552
Conjugate/TagUnconjugated
Antibody TypePrimary
Quantity10 UL, 40 UL, 100 UL
HostRabbit
ClonalityMonoclonal
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
IsotypeIgG
CloneEPR3821
Concentration0.189 mg/ml
Gene NamePCNA
ReactivityHuman, Mouse, Rat
TargetPCNA

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) CSTF2T facilitates pancreatic adenocarcinoma growth and metastasis by elevating H3K4Me1 methylation of CALB2 via ASH2LCancer Biology & TherapyDecember 31, 2023Wei Liu, Dongwei Hu, Jinjie Li, Qigang Xu, Zongjing Chen, Bo XuCALB2 (PAB20373, 1:1000, Amyjet Scientific, Wuhan, China), proliferating cell nuclear antigen (PCNA, ab92552, 1:1000, Abcam), cleaved-Caspase3 (ab32042, 1:500, Abcam), cleaved-Caspase8 (ABP50008, 1:500, Amyjet

(2) PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancerJ NanobiotechnologyDecember 11, 2023Chao Wang, Yong-Hong Xu, Hua-Zhen Xu, Ke Li, Quan Zhang, Lin Shi, Li Zhao, Xiao Chenimmunohistochemical (IHC) staining. IHC analysis Antibodies for immunohistochemical analysis included PCNA (ab92552, Abcam), Ki67 (ab16667, Abcam), cle-caspase 3 (9664, CST), PD-L1 (66248-1-Ig, Proteintech), CD206 (ab64693,

(3) Prevention of STAT3-related pathway in SK-N-SH cells by natural product astaxanthinBMC Complementary Medicine and TherapiesNovember 29, 2023Shao-Qian Sun, Feng-Xiang Du, Li-Hua Zhang, Hao-Shi, Fu-Ying Gu, Yu-Lin Deng, Yi-Zhi Jianti-Caspase-9 (1:1000, ab32539, Abcam), anti-NF-kβp65 (1:1000, ab76302, Abcam), anti-JAK2 (1:1000, ab92552, Abcam), anti-Stat3 (1:1000, ab119352, Abcam), and anti-β-actin (1:1000, ab8227, Abcam). Following primary

(4) Bisdemethoxycurcumin alleviates LPS-induced acute lung injury via activating AMPKα pathwayBMC Pharmacology and ToxicologyNovember 20, 2023Huifang Li, Qi Zou, Xueming Wangwere obtained from Cell Signaling Technology (Danvers, MA, USA). The anti-SOD2 (#ab68155), anti-PCNA (#ab92552) and NRF2 Transcription Factor Assay Kit (#ab207223) were purchased from Abcam (Cambridge, MA, USA).

(5) Inhibitory Effect of β-Sitosterol on the Ang II-Induced Proliferation of A7r5 Aortic Smooth Muscle CellsAnalytical Cellular PathologyNovember 7, 2023Yuankun Chen, Shumiao He, Ao Zeng, Siqing He, Xiaobao Jin, Chunmei Li, Wenjie Mei, Qun Luantibody (ab214050), anti-α-SMA antibody (ab5694), anti-LC3B antibody (ab192890), anti-PCNA antibody (ab92552), anti-Cyclin D1 antibody (ab40754), anti-Beclin 1 antibody (ab210498), and the secondary antibody (ab6721)

(6) Bone Marrow Mesenchymal Stem Cells Release miR-378a-5p-Carried Extracellular Vesicles to Alleviate Rheumatoid ArthritisJournal of Innate ImmunityNovember 3, 2023Yaqin Zhang, Ziying Jiao, Shanshan WangAbcam, UK), Bcl-2 (1:1,000, ab32124, Abcam, UK), Ki67 (1:5,000, ab16667, Abcam, UK), PCNA (1:5,000, ab92552, Abcam, UK), YAP (1:10,000, ab76252, Abcam, UK), VEGFA (1:1,000, ab46154, Abcam, UK), E-cadherin (1:5,000,

(7) In vitro study of miRNA-369-3p targeting TCF4 regulating the malignant biological behavior of colon cancer cellsJournal of Gastrointestinal OncologyOctober 31, 2023Yushan Li, Jianming Sun, Angelica Judith Granados-López, Zhiyue Chu, Hong Zhangcat. no. ab217668; Abcam, UK), Ki67 (1/5,000; cat. no. ab92742; Abcam, UK), PCNA (1/1,000; cat. no. ab92552; Abcam, UK), vascular endothelial growth factor (VEGF) (1 µg/mL; cat. no. ab46154; Abcam, UK), E-cadherin

(8) Paternal Obesity Induced by High-Fat Diet Impairs the Metabolic and Reproductive Health of Progeny in RatsMetabolitesOctober 20, 2023Carlos Larqué, Haydée Lugo-Martínez, Xiadany Mendoza, Monserrat Nochebuena, Luis Novo, Ricardo Vilchis, Guadalupe Sánchez-Bringas, Laura Ubaldo, Myrian Velasco, Rene EscalonaSanta Cruz, TX, USA); mouse anti-WT1 (1:100; SC-7385, Santa Cruz, TX, USA); and rabbit anti PCNA (1:500; ab92552, Abcam; Cambridge, UK). The slides were washed twice in PBS-0.05% Tween and incubated 2 h at RT with

(9) Circ_0102231 inactivates the PI3K/AKT signaling pathway by regulating the miR‐635/NOVA2 pathway to promote the progression of non‐small cell lung cancerThoracic CancerOctober 20, 2023Jianhong Liu, Qiong Yu, Xu Yangdetection system (Thermo Fisher) was applied to visualize the bands. The antibodies were: anti‐PCNA (ab92552, Abcam), anti‐NOVA2 (PA5‐69076, Thermo Fisher), anti‐p‐PI3K (ab278545, Abcam), anti‐p‐AKT (ab38449,

(10) Triptolide inhibits epithelial ovarian tumor growth by blocking the hedgehog/Gli pathwayAging (Albany NY)October 17, 2023Lanyan Hu, Mai Gao, Huifu Jiang, Lingling Zhuang, Ying Jiang, Siqi Xie, Hong Zhang, Qian Wang, Qi Chenantibodies including Smo (ab72130), E-cadherin (ab1416), N-cadherin (ab18203), MMP9 (ab76003), PCNA (ab92552) and Ki-67 (ab16667) were obtained from Abcam (UK). Cell culture A2780 cells were obtained from ATCC

(11) Bone-targeting exosome nanoparticles activate Keap1 / Nrf2 / GPX4 signaling pathway to induce ferroptosis in osteosarcoma cellsJournal of NanobiotechnologySeptember 30, 2023Wenkai Chen, Zongguang Li, Naichun Yu, Linlin Zhang, Hongyu Li, Yongjie Chen, Fengqing Gong, Wenping Lin, Xu He, Siyuan Wang, et al.Cat#ab75972, Abcam), Keap1(1:1000, Cat#ab227828, Abcam), Nrf2(1:1000, Cat#ab62352, Abcam), PCNA(1:5000, Cat#ab92552, Abcam). Cellular GSH levels The GSH measurement was conducted by a kit, per the protocols (Beyotime,

(12) DNA-Based Hydrogels with Multidrug Sequential Release for Promoting Diabetic Wound RegenerationJACS AuSeptember 25, 2023Wei Li, Hui Xie, Liping Gou, Ye Zhou, Hao Wang, Ruoqing Li, Yong Zhang, Shuyun Liu, Jingping Liu, Yanrong Lu, et al.anti-iNOS (ab202417, 1:1000, Abcam), anti-mannose receptor (CD206, ab300621, 1:1000, Abcam), anti-PCNA (ab92552, 1:1000, Abcam), anti-CD31 (ab222783, 1:1000, Abcam), anti-α-SMA (55135-1-AP, 1:1000, Proteintech),

(13) The application of HER2 and CD47 CAR-macrophage in ovarian cancerJournal of Translational MedicineSeptember 22, 2023Yizhao Chen, Xiangling Zhu, Hanze Liu, Cunzhi Wang, Yu Chen, Huihui Wang, Yilong Fang, Xuming Wu, Yuting Xu, Chunhua Li, et al.standard protocol. The primary antibodies were included: anti-proliferating cell nuclear antigen (PCNA) (ab92552, Abcam), MHC II (ab180779, Abcam), CD163 (ab182422, Abcam), IFN-γ (ab218426, Abcam), HER2 (ab134182,

(14) Quercetin inhibits the metabolism of arachidonic acid by inhibiting the activity of CYP3A4, thereby inhibiting the progression of breast cancerMolecular MedicineSeptember 14, 2023Huaming Tang, Yuanli Kuang, Wan Wu, Bing Peng, Qianmei Fualbumin (BSA) and incubated with corresponding primary antibodies to CYP3A4 (ab230022, Abcam), PCNA (ab92552, Abcam), p21 (ab109199, Abcam), caspase3 (ab32351, Abcam), cleaved caspase3 (ab2302, Abcam), PARP (9542,

(15) METTL14 mediates m(6)a modification on osteogenic proliferation and differentiation of bone marrow mesenchymal stem cells by regulating the processing of pri-miR-873Molecular medicine reportsSeptember 1, 2023Dong X, Liao B, Zhao J, Li X, Yan K, Ren K, Zhang X, Bao X, Guo W.room temperature, followed with incubation with primary antibodies against PCNA (1:1,000; cat. no. ab92552; Abcam), osteocalcin (1:2,000; cat. no. sc-376835; Santa Cruz), Bglap (1:1,000; cat. no. MBS3223783;

(16) IGF2-NR4A2 Signaling Regulates Macrophage Subtypes to Attenuate Liver CirrhosisJournal of Clinical and Translational HepatologyAugust 28, 2023Lichao Yao, Xue Hu, Mengqin Yuan, Qiuling Zhang, Pingji Liu, Lian Yang, Kai Dai, Yingan Jiangovernight with primary antibodies, anti-Ki67 (ab16667, 1:200; Abcam, Cambridge, UK) and anti-PCNA (ab92552, 1:200; Abcam) at 4 °C. After the sections were washed and incubated for 1 h with horseradish peroxidase-conjugated

(17) Akkermansia muciniphila inhibits tryptophan metabolism via the AhR/β-catenin signaling pathway to counter the progression of colorectal cancerInternational Journal of Biological SciencesAugust 21, 2023Lu Zhang, Qing Ji, Qian Chen, Zhenzhen Wei, Shuochuan Liu, Long Zhang, Yuli Zhang, Zan Li, Huaimin Liu, Hua Suiat 37°C in a humidified 5% CO2 atmosphere. Monoclonal antibodies specific for Ki67 (ab1667), PCNA (ab92552), AhR (83200S), β-catenin (8480S), LEF1 (2230S), TCF4 (2569S), GAPDH (5174S) and β-actin (ab179467)

(18) Highly reproducible rat arterial injury model of neointimal hyperplasiaPLoS OneAugust 17, 2023Richard P. Tan, Jui Chien Hung, Alex H. P. Chan, Angus J. Grant, Matthew J. Moore, Yuen Ting Lam, Praveesuda Michael, Steven G. Wiseand PCNA for smooth muscle cell contractile and synthetic phenotype respectively, and CD68 (Abcam, ab92552, 1:500), MHC Class II (Abcam, ab180779, 1:500), IL-1β (ThermoFisher, P420B, 1:200), and TNF-α (Abcam,

(19) Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate paclitaxel-induced spermatogenesis defects and maintain male fertilityBiological ResearchAugust 13, 2023YuSheng Zhang, YaNan Liu, Zi Teng, ZeLin Wang, Peng Zhu, ZhiXin Wang, FuJun Liu, XueXia Liu70 kDa Protein 2 (HSPA2) (ab108416), HSPA4L (ab231577), Proliferating cell nuclear antigen (PCNA) (ab92552), Sirtuin 1 (SIRT1) (ab189494, Abcam, Cambridge, UK), STEM121 (Y40410, Cellartis, Takara, Japan), and

(20) Epicardial Adipose Tissue–Derived Leptin Promotes Myocardial Injury in Metabolic Syndrome Rats Through PKC/NADPH Oxidase/ROS PathwayJournal of the American Heart AssociationAugust 1, 2023Hui Chen, Lei Liu, Min Li, Danjun Zhu, Gang Tianc (catalog number ab133504, 1:1000), COX IV (catalog number ab202554, 1:2000), PCNA (catalog number ab92552, 1:5000), and β‐actin (catalog number ab133504, 1:5000) (all from Abcam). Equivalent amounts of the

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Anti-PCNA antibody [EPR3821] from Abcam

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Anti-PCNA antibody [EPR3821] from Abcam

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