Mouse IgG2a kappa Alexa Fluor 488 Isotype Control 53-4724-80 from Thermo Fisher Scientific

Product Specs
ItemMouse IgG2a kappa Alexa Fluor 488 Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number53-4724-80
Quantity25 µg
ApplicationsControl, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Concentration0.5 mg/mL
Conjugate/TagAlexa Fluor™ 488
FormatPBS with 0.09% sodium azide; pH 7.2
TargetMouse IgG2a kappa
IsotypeIgG2a kappa
HostMouse
PurityAffinity chromatography
CloneeBM2a
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypeIsotype Control
Storage4°C, store in dark, DO NOT FREEZE!
ClonalityMonoclonal

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(1) Lipophosphoglycan From Dermotropic New World Leishmania Upregulates Interleukin-32 and Proinflammatory Cytokines Through TLR4 and NOD2 ReceptorsFrontiers in cellular and infection microbiologyJanuary 1, 2022Silveira MB, Gomes RS, Shio MT, Rugani JN, Paranaiba LF, Soares RP, Ribeiro-Dias F.53-9917-42) or isotype control (IgG2a K mouse Alexa Fluor 488, Clone eBM2a, eBioscience, catalog n. 53-4724-80), for 15 min, to analyze TLR4 expression on the cell membrane. Cells were washed twice with PBS and

(2) Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 ProductionFrontiers in immunologyJanuary 1, 2019Faraj TA, Stover C, Erridge C.anti-TLR2, anti-TLR4 and mouse IgG2a-K isotype control (eBioscience, 53-9922-41, 1:20, 53-9917-41, 1:20 & 53-4724-80, 1:50). Data were acquired using a Gallios cytometer (Beckman Coulter). Hepatocyte and Hepatic Macrophage

(3) Resistin facilitates metastasis of lung adenocarcinoma through the TLR4/Src/EGFR/PI3K/NF-κB pathwayCancer scienceAugust 1, 2018Gong WJ, Liu JY, Yin JY, Cui JJ, Xiao D, Zhuo W, Luo C, Liu RJ, Li X, Zhang W, et al.488 (E10289‐1632; eBioscience, San Diego, CA, USA) or mouse IgG2a k isotype control Alexa Fluor 488 (53472480, eBioscience) in the dark for 30 min on ice and detected by an FC500 Flow Cytometer. 2.7 Cell migration

(4) Dengue immune sera enhance Zika virus infection in human peripheral blood monocytes through Fc gamma receptorsPloS oneJanuary 1, 2018Li M, Zhao L, Zhang C, Wang X, Hong W, Sun J, Liu R, Yu L, Wang J, Zhang F, et al.

(5) Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal CellsStem cell reportsMay 10, 2016Lindsay SL, Johnstone SA, McGrath MA, Mallinson D, Barnett SC.for 20 min, followed by TLR4-488 (eBioscience; 53-9917-41) or mouse isotype control 488 (eBioscience; 53-4724-80) for 20 min on ice. Cells were washed then resuspended in PBS containing 2% paraformaldehyde and evaluated

(6) TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass SurgeryPloS oneJanuary 1, 2015Avlas O, Bragg A, Fuks A, Nicholson JD, Farkash A, Porat E, Aravot D, Levy-Drummer RS, Cohen C, Shainberg A, et al.this separated cell fraction was examined by FACS analysis using Mouse IgG2a IsoControl (eBioscience #53-4724-80) and Anti-human CD284 (TLR4) Clone HTA125 (eBioscience #53-9917-41) or Anti-human CD282 (TLR2) Clone

(7) Iron sucrose impairs phagocytic function and promotes apoptosis in polymorphonuclear leukocytesAmerican journal of nephrologyJanuary 1, 2012Ichii H, Masuda Y, Hassanzadeh T, Saffarian M, Gollapudi S, Vaziri ND.

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10/17/2019A Good Isotype Antibody for Assessing Non-Specific Antibody Binding on Peripheral CD8+ T Cells by Flow Cytometry Chris Corkum,Highly recommended for use as an isotype control in flow cytometry experiments involving PBMC samples or purified CD8+ T cells when properly matched to a test antibody.

Mouse IgG2a kappa Alexa Fluor 488 Isotype Control from Thermo Fisher Scientific

Thermo Fisher Scientific

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Mouse IgG2a kappa Alexa Fluor 488 Isotype Control from Thermo Fisher Scientific

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