C57BL/6 mouse resident peritoneal exudate cells were surface stained with F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-4801-82). The cells were then fixed and permeabilized using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, and intracellularly stained with either 0.25 µg of Rat IgG1 kappa Isotype Control, PE (Product # 12-4301-82) (left) or 0.25 µg of RELM alpha Monoclonal Antibody, PE (Product # 12-5441-82)(right). All cells were used for analysis.