IFN gamma Antibody PerCP-Cyanine5.5 45-7311-82 from Thermo Fisher Scientific

Product Specs
ItemIFN gamma Antibody PerCP-Cyanine5.5
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number45-7311-82
Quantity100 µg
ApplicationsFlow Cytometry
Concentration0.2 mg/mL
Conjugate/TagPerCP-Cyanine5.5
FormatPBS with 0.09% sodium azide; pH 7.2
ReactivityMouse
TargetIFN gamma
IsotypeIgG1 kappa
CloneXMG1.2
HostRat
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFN-g, If2f, Ifg
ClonalityMonoclonal
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypePrimary
Storage4° C, store in dark, DO NOT FREEZE!

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Self-amplifying mRNA seasonal influenza vaccines elicit mouse neutralizing antibody and cell-mediated immunity and protect ferretsnpj VaccinesOctober 4, 2023Michael Cheung, Cheng Chang, Raveen Rathnasinghe, Evan Rossignol, Yunfei Zhang, Annette Ferrari, Harsh Patel, Yanjun Huang, Michelle Sanchez Guillen, Tina Scalzo, et al.Fluor 488-labeled anti-TNFα (1:160, BD Biosciences #557719), PerCP/Cy5.5-labeled anti-IFN-γ (1:160, #45-7311-82, eBioscience, San Diego, California, USA), allophycocyanin-labeled anti-IL-5 (1:80, #504306, BioLegend,

(2) Icariin restrains NLRP3 inflammasome-mediated Th2 immune responses and ameliorates atopic dermatitis through modulating a novel lncRNA MALAT1/miR-124-3p axisPharm BiolAugust 21, 2023Wei Zhao, Huan-Huan Yu, Wei-Wei Meng, Ai-Min Liu, Bu-Xin Zhang, Ying Wang, Jie Li, Li Wang, Yu-Fu Fangthe instructions, and then stained with anti-IFN-γ-PerCP-cy5.5 (Invitrogen, Carlsbad, CA, cat. no. 45-7311-82, 1/800 dilution) and anti-IL-4-PE (Invitrogen, Carlsbad, CA, cat. no. 12-7041-82, 1/160 dilution) for

(3) IL-18BP mediates the balance between protective and pathological immune responses to Toxoplasma gondiiCell reportsMarch 28, 2023Clark JT, Weizman OE, Aldridge DL, Shallberg LA, Eberhard J, Lanzar Z, Wasche D, Huck JD, Zhou T, Ring AM, et al."IFN gamma Monoclonal Antibody (XMG1.2), PerCP-Cyanine5.5, eBioscience™",Thermo Fisher,"(Thermo Fisher Scientific Cat# 45–7311-82, RRID:AB_1107020)" "CD200 Receptor Monoclonal Antibody (OX110), APC, eBioscience™",Biolegend,"(Thermo Fisher Scientific

(4) TCR repertoire landscape reveals macrophage-mediated clone deletion in endotoxin toleranceInflammation ResearchMarch 1, 2023Juanjuan Zhao, Li Jia, YiJing Tao, Xu Zhao, Jing Yang, Yanxin Lu, Yaping Yan, Ling Mao, Lin Hu, Jia Lu, et al.CD4-Percp-Cy5.5 (no. 12–0041-82), CD62L-PE (no. 12–0621-81), CD69-APC (no. 17–0691-82), IFN-γ-Percp-Cy5.5 (no. 45–7311-82), IL-4-APC (no. 17–7041-82), and Ki67-PE (no. 12–5698-82), with corresponding isotype-matched controls

(5) Differential requirement of neutralizing antibodies and T cells on protective immunity to SARS-CoV-2 variants of concernNPJ vaccinesFebruary 13, 2023Azevedo PO, Hojo-Souza NS, Faustino LP, Fumagalli MJ, Hirako IC, Oliveira ER, Figueiredo MM, Carvalho AF, Doro D, Benevides L, et al.Cytofix/Cytoperm (BD Bioscience) for 20 min at 4 °C in the dark and incubated with anti-IFN-γ (PerCP-Cy5.5; Cat. # 45-7311-82; 1:80; eBioscience) for 30 min at 4 °C. Representative density plots are presented in Supplementary

(6) T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasisImmunityJanuary 10, 2023Briukhovetska D, Suarez-Gosalvez J, Voigt C, Markota A, Giannou AD, Schübel M, Jobst J, Zhang T, Dörr J, Märkl F, et al.

(7) Biometabolites of Citrus unshiu Peel Enhance Intestinal Permeability and Alter Gut Commensal BacteriaNutrientsJanuary 9, 2023Lee SH, Seo D, Lee KH, Park SJ, Park S, Kim H, Kim T, Joo IH, Park JM, Kang YH, et al.

(8) The aryl hydrocarbon receptor cell intrinsically promotes resident memory CD8+ T cell differentiation and functionCell ReportsJanuary 4, 2023Joseph W. Dean, Eric Y. Helm, Zheng Fu, Lifeng Xiong, Na Sun, Kristen N. Oliff, Marcus Muehlbauer, Dorina Avram, Liang ZhouQA16A02),BioLegend,Cat# 372222; RRID: AB_2728389 Anti-mouse IFN gamma – PerCP-Cyanine5.5 (clone XMG1.2),Invitrogen,Cat# 45-7311-82; RRID: AB_1107020 Anti-mouse TNF – PE-Cyanine7 (clone MP6-XT22),Invitrogen,Cat# 25-7321-82; RRID: AB_11042728 Anti-mouse

(9) Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial CellsCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Zhang Q, Su X, Zhang C, Chen W, Wang Y, Yang X, Liu D, Zhang Y, Yang R.

(10) Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challengeBiomaterialsJanuary 1, 2023Sun S, Li E, Zhao G, Tang J, Zuo Q, Cai L, Xu C, Sui C, Ou Y, Liu C, et al.

(11) Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the braineLifeDecember 21, 2022Kovacs MA, Cowan MN, Babcock IW, Sibley LA, Still K, Batista SJ, Labuzan SA, Sethi I, Harris TH.the following eBioscience antibodies at 1:200 dilution: TNF-α-AF488 (53-7321-82), IFN-γ-PerCP-Cy5.5 (45-7311-82), and Ki67-PE-Cy7 (25-5698-82). The following eBioscience live/dead dyes were used at a 1:800 dilution:

(12) Local and Systemic Injections of Human Cord Blood Myeloid-Derived Suppressor Cells to Prevent Graft Rejection in Corneal TransplantationBiomedicinesDecember 12, 2022Lee JY, Sohn HJ, Kim CH, Kim TG, Lee HS.

(13) Agr2-associated ER stress promotes adherent-invasive E. coli dysbiosis and triggers CD103(+) dendritic cell IL-23-dependent ileocolitisCell reportsNovember 15, 2022Viladomiu M, Khounlotham M, Dogan B, Lima SF, Elsaadi A, Cardakli E, Castellanos JG, Ng C, Herzog J, Schoenborn AA, et al.

(14) Transcription factor RORα enforces stability of the Th17 cell effector program by binding to a Rorc cis-regulatory elementImmunityNovember 8, 2022Hall JA, Pokrovskii M, Kroehling L, Kim BR, Kim SY, Wu L, Lee JY, Littman DR.

(15) Expansion of T memory stem cells with superior anti-tumor immunity by Urolithin A-induced mitophagyImmunityNovember 8, 2022Denk D, Petrocelli V, Conche C, Drachsler M, Ziegler PK, Braun A, Kress A, Nicolas AM, Mohs K, Becker C, et al.

(16) RhoB affects colitis through modulating cell signaling and intestinal microbiomeMicrobiomeSeptember 16, 2022Yang J, Pei G, Sun X, Xiao Y, Miao C, Zhou L, Wang B, Yang L, Yu M, Zhang ZS, et al.anti-IL-17A conjugated to APC (1:100, 509616, Biolegend), anti-IFN-γ conjugated to PerCP/Cy5.5 (1:100, 45-7311-82, Invitrogen), anti-IL-4 conjugated to PE (1:100, 504103, Biolegend). For Treg cell analysis, cells were

(17) Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 ReceptormBioAugust 30, 2022Akhtar MN, Kumar S, Sen P.

(18) Dendritic cell Piezo1 directs the differentiation of T(H)1 and T(reg) cells in cancereLifeAugust 22, 2022Wang Y, Yang H, Jia A, Wang Y, Yang Q, Dong Y, Hou Y, Cao Y, Dong L, Bi Y, et al.

(19) Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus InfectionInternational journal of molecular sciencesAugust 20, 2022Yang G, Wang J, Wang S, Zhu Y.

(20) Breast cancer cell-derived extracellular vesicles promote CD8(+) T cell exhaustion via TGF-β type II receptor signalingNature communicationsAugust 1, 2022Xie F, Zhou X, Su P, Li H, Tu Y, Du J, Pan C, Wei X, Zheng M, Jin K, et al.

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(1)

11/21/2020Great IFNg antibody in PerCP Meng SunWhen our lab does adoptive transfer cells into a host and then infect the host with an acute infection (like LCMV Armstrong). When we harvest the lymphocytes from their spleens, we always re-stimulate... Read Review
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12/6/2019IFN Gamma Monoclonal Antibody (XMG1.2), PerCP-Cyanine5.5 Christoforos DimitropoulosGood, sensitive, high-resolution antibody.
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1/16/2019Great Flow Antibody to Detect IFNg Secretion in T CellsDr. Min Hee OhAs an immunologist, we assess CD8 T cell cytokine secretion via flow cytometry. We analyze interferon gamma secretion (IFNg) since this cytokine is one of the first cytokines to be expressed for ... Read Review
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8/17/2018Great Antibody to Identify IFNG+ Secreting T Cells Chirag PatelOur lab studies the mTOR signaling pathway in T cell differentiation with an emphasis on both CD4 and CD8 T cells. We routinely conduct flow cytometry experiments to analyze the differentiation ... Read Review

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IFN gamma Antibody PerCP-Cyanine5.5 from Thermo Fisher Scientific

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IFN gamma Antibody PerCP-Cyanine5.5 from Thermo Fisher Scientific

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