Fig 1: Rb1 attenuates oxidative stress and suppresses inflammation in PI. (A) ELISA quantification of serum TNF-α, IL-6, and IL-10 levels; (B) ELISA detection of TNF-α, IL-6, and IL-10 levels in homogenates of dorsal wound tissues; (C) immunohistochemical analysis of TNF-α, IL-6, and IL-10 expressions in dorsal wound tissues (bar: 25 μm); (D) quantitative analysis of inflammatory cytokines across groups; (E) immunohistochemical analysis of CD8, CD80, and CD163 expressions in dorsal wound tissues. Scale bar: 25 μm; (F) quantification of CD8, CD80, and CD163 expressions across different treatment groups; (G) quantification of MDA levels in different treatment groups; (H) quantification of GSH levels in different treatment groups; (I) immunohistochemical analysis of 4-HNE expression in dorsal wound tissues (Scale bar: 25 μm). Each group contained six animals. All data are presented as mean ± standard deviation (Mean ± SD). Comparisons between two groups were performed using an independent-sample t-test. *p < 0.05, **p < 0.01, and ***p < 0.001 compared between groups.