MHC Class II (I-A/I-E) Antibody Alexa Fluor 700 56-5321-82 from Thermo Fisher Scientific

Product Specs
ItemMHC Class II (I-A/I-E) Antibody Alexa Fluor 700
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number56-5321-82
Quantity100 µg
ApplicationsFlow Cytometry
Concentration0.2 mg/mL
Conjugate/TagAlexa Fluor™ 700
FormatPBS with 0.09% sodium azide; pH 7.2
ReactivityMouse
TargetMHC Class II (I-A/I-E)
IsotypeIgG2b kappa
CloneM5/114.15.2
HostRat
NCBI Full Gene Namehistocompatibility 2, class II antigen A, beta 1, histocompatibility 2, class II antigen E beta
NCBI Gene AliasesH-2Eb, Rmcs1, Ia2, I-Abeta, IAb, Eb, H-2Ab, Abeta, H2Eb, Ia-4, H2-Ab, Ia4, Ia-2
ClonalityMonoclonal
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypePrimary
Storage4° C, store in dark, DO NOT FREEZE!

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) B Cells from Aged Mice Do Not Have Intrinsic Defects in Affinity Maturation in Response to ImmunizationThe Journal of ImmunologyNovember 15, 2023Jia Le Lee, Silvia Innocentin, Alyssa Silva-Cayetano, Stephane M. Guillaume, Michelle A. Lintermananti-mouse CD40,BioLegend (3/23),124622,1:2000 A700-coupled anti-mouse MHCII IA/IE,Invitrogen (M5/114.15.2),56-5321-82,1:2000 ViaKrome808-coupled Live/Dead,Beckman Coulter,C36628,1:2000 APC-Fire750-coupled anti-mouse

(2) Circular RNA vaccine induces potent T cell responsesProceedings of the National Academy of Sciences of the United States of AmericaMay 16, 2023Amaya L, Grigoryan L, Li Z, Lee A, Wender PA, Pulendran B, Chang HY.127624), anti-CD19 (BUV395, BD 563557), anti-CD3 (BB700, BD742175), anti-MHCII (AF700, eBioscience 56-5321-82), anti-CD11b (BV650, BioLegend 101239), anti-CD11c (BV421, BioLegend 117330), anti-CD86 (A647, BioLegend

(3) FGF21 protects against hepatic lipotoxicity and macrophage activation to attenuate fibrogenesis in nonalcoholic steatohepatitiseLifeJanuary 17, 2023Cong Liu, Milena Schönke, Borah Spoorenberg, Joost M Lambooij, Hendrik J P van der Zande, Enchen Zhou, Maarten E Tushuizen, Anne-Christine Andreasson, Andrew Park, Stephanie Oldham, et al.700,BioLegend,105319 CLEC2,17D9,FITC,Bio-Rad,MCA5700 F4/80,BM8,BV711,BioLegend,123147 Ly6C,HK1.4,APC-Cy7,BioLegend,128025 Ly6G,1A8,BV650,BioLegend,127641 MHC-II,M5/114.15.2,BV750,BD Biosciences,747458 MHC-II,M5/114.15.2,Alexa Fluor 700,Thermo Fisher,56-5321-82 NK1.1,PK136,PerCP-Cy5.5,BioLegend,108727 Siglec-F,E50-2440,PE,BD Biosciences,552126 Siglec-F,E50-2440,BV605,BD Biosciences

(4) Adjuvanting a subunit SARS-CoV-2 vaccine with clinically relevant adjuvants induces durable protection in micenpj VaccinesDecember 1, 2022Lilit Grigoryan, Audrey Lee, Alexandra C. Walls, Lilin Lai, Benjamin Franco, Prabhu S. Arunachalam, Yupeng Feng, Wei Luo, Abigail Vanderheiden, Katharine Floyd, et al.566411, 1:200 dilution), anti-CD3 (BB700; BD #742175, 1:200 dilution), anti-MHCII (AF700; eBioscience #56-5321-82, 1:400 dilution), anti-CD11b (BV650; Biolegend #101239, 1:300 dilution), anti-CD11c (BV421; Biolegend

(5) CDKN1A is a target for phagocytosis-mediated cellular immunotherapy in acute leukemiaNature communicationsNovember 8, 2022Allouch A, Voisin L, Zhang Y, Raza SQ, Lecluse Y, Calvo J, Selimoglu-Buet D, de Botton S, Louache F, Pflumio F, et al.anti-mF4/80 clone BM8 (#17-4801-82, diluted 1:100), Alexa Fluor 700 anti-mMHCII (I-A/I-E) clone M5/114.15.2 (#56-5321-82, diluted 1:100), and PE-Cyanine7 anti-hCD71 clone OKT9 (#25-0719-42, diluted 1:100) were purchased from

(6) Do more with Less: Improving High Parameter Cytometry Through Overnight StainingCurr ProtocNovember 1, 2022Whyte CE, Tumes DJ, Liston A, Burton OT.780,GL3,1:200,13,ThermoFisher,78‐5711‐82 Anti‐mouse I‐1/I‐E Alexa Fluor 700,M5/114.15.2,1:500,13,ThermoFisher,56‐5321‐82 Anti‐mouse CD11c Alexa Fluor 488,N418,1:1000,13,ThermoFisher,53‐0114‐82 Reagents Used for This Study

(7) A RORγt(+) cell instructs gut microbiota-specific T(reg) cell differentiationNatureOctober 1, 2022Kedmi R, Najar TA, Mesa KR, Grayson A, Kroehling L, Hao Y, Hao S, Pokrovskii M, Xu M, Talbot J, et al.(RMV-7), BD 747834, 1:100;CD51 TotalSeq™-A1008 (RMV-7), BioLegend 104111, 1:100;MHCII I-A/I-E AF700 (56-5321-82), eBioscience 56-5321-80, 1:200;MHCII I-A/I-E BV421 (M5/114.15.2), BioLegend 107631, 1:400;CCR6 BV711(140706),

(8) Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in miceSTAR ProtocolsSeptember 16, 2022Michael R. Dâ€™Agostino, Sam Afkhami, Alisha Kang, Art Marzok, Matthew S. Miller, Zhou Xing550261,RRID: AB_398460" Anti-mouse MHC II AF700 (clone M5/114.15.2),Thermo Fisher Scientific,"Cat# 56-5321-82,RRID: AB_494009" Anti-mouse CD3 V450 (clone 17A2),BD Biosciences,"Cat# 561389,RRID: AB_10679120" Anti-mouse

(9) An improved organotypic cell culture system to study tissue-resident macrophages ex vivoCell Rep MethodsAugust 22, 2022Aktories P, Petry P, Glatz P, Andrieux G, Oschwald A, Botterer H, Gorka O, Erny D, Boerries M, Henneke P, et al.RRID: AB_493523 "Alexa Fluor 700 anti-mouse I-A/I-E Antibody, Clone M5/114.15.2",Thermo Fisher,Cat # 56-5321-82;RRID: AB_494009 "PE anti-mouse Tim-4 Antibody, Clone RMT4-54",BioLegend,Cat# 130006; RRID: AB_2201843 "APC anti-mouse Tim-4

(10) Toll-like Receptor 9 Pathway Mediates Schlafen⁺-MDSC Polarization During Helicobacter-induced Gastric MetaplasiasGastroenterologyAugust 1, 2022Ding L, Chakrabarti J, Sheriff S, Li Q, Thi Hong HN, Sontz RA, Mendoza ZE, Schreibeis A, Helmrath MA, Zavros Y, et al.PDCA-1 (Biolegend, #127103), IFNα (Abcam, #ab7373), CD11b (Biolegend #101239), MHCII (ThermoFisher # 56-5321-82). Human DCs, MDSCs and CTLs in the dish were harvested in PBS and stained with SLFN12L (Novus Biological

(11) RXRα Regulates the Development of Resident Tissue MacrophagesImmunoHorizonsJune 22, 2022Philpott J, Kazimierczyk S, Korgaonkar P, Bordt E, Zois J, Vasudevan C, Meng D, Bhatia I, Lu N, Jimena B, et al.Fluor 647 (429, BioLegend, 105712), MHC class II Alexa Fluor 700 (M5/114, Thermo Fisher Scientific, 56532182), Siglec-F BV421 (E50-2440, BD Biosciences, 562681), Tim-4 BV510 (21H12, BD Biosciences, 742774), Live/Dead,

(12) Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccineNature immunologyApril 1, 2022Li C, Lee A, Grigoryan L, Arunachalam PS, Scott MKD, Trisal M, Wimmers F, Sanyal M, Weidenbacher PA, Feng Y, et al.(1:100, BB700; BD #566411), anti-CD3 (1:100, BB700; BD #742175), anti-MHCII (1:400, AF700; eBioscience #56-5321-82), anti-CD11b (1:300, BV650; BioLegend #101239), anti-CD11c (1:400, BV421; BioLegend #117330), anti-CD86

(13) Interferon-γ primes macrophages for pathogen ligand-induced killing via a caspase-8 and mitochondrial cell death pathwayImmunityMarch 8, 2022Simpson DS, Pang J, Weir A, Kong IY, Fritsch M, Rashidi M, Cooney JP, Davidson KC, Speir M, Djajawi TM, et al.(FITC),eBioscience,Cat#: 11-4801-81; RRID: AB_2735037; Clone: BM8 Rat anti-MHCII (AF700),eBioscience,Cat#: 56-5321-82; RRID: AB_494009; Clone: M5/114.15.2 Rat anti-CD206 (APC),eBioscience,Cat#: 47-2061-82; RRID: AB_2802285;

(14) Respiratory mucosal delivery of next-generation COVID-19 vaccine provides robust protection against both ancestral and variant strains of SARS-CoV-2CellMarch 3, 2022Afkhami S, D'Agostino MR, Zhang A, Stacey HD, Marzok A, Kang A, Singh R, Bavananthasivam J, Ye G, Luo X, et al.550261, RRID:AB_398460" Anti-mouse MHC II AF700 (clone M5/114.15.2),Thermo Fisher Scientific,"Cat# 56-5321-82, RRID:AB_494009" Anti-mouse CD3 V450 (clone 17A2),BD Biosciences,"Cat# 561389, RRID:AB_10679120" Anti-mouse

(15) A molecular atlas of innate immunity to adjuvanted and live attenuated vaccines, in miceNature communicationsJanuary 27, 2022Lee A, Scott MKD, Wimmers F, Arunachalam PS, Luo W, Fox CB, Tomai M, Khatri P, Pulendran B.(1:100; BB700; BD #566411), anti-CD3 (1:100; BB700; BD #742175), anti-MHCII (1:400; AF700; eBioscience #56-5321-82), anti-CD11b (1:300; BV650; Biolegend #101239), anti-CD11c (1:400; BV421; Biolegend #117330), anti-CD86

(16) Transgene distribution and immune response after ultrasound delivery of rAAV9 and PHP.B to the brain in a mouse model of amyloidosisMolecular therapy. Methods & clinical developmentDecember 10, 2021Kofoed RH, Heinen S, Silburt J, Dubey S, Dibia CL, Maes M, Simpson EM, Hynynen K, Aubert I.

(17) Skin inflammation activates intestinal stromal fibroblasts and promotes colitisThe Journal of clinical investigationNovember 1, 2021Dokoshi T, Seidman JS, Cavagnero KJ, Li F, Liggins MC, Taylor BC, Olvera J, Knight R, Chang JT, Salzman NH, et al.

(18) Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2Cell death discoveryOctober 29, 2021Zhou N, Liu W, Zhang W, Liu Y, Li X, Wang Y, Zheng R, Zhang Y.

(19) Natural killer cells and dendritic epidermal γδ T cells orchestrate type 1 conventional DC spatiotemporal repositioning toward CD8⁺ T cellsiScienceSeptember 24, 2021Ghilas S, Ambrosini M, Cancel JC, Brousse C, Massé M, Lelouard H, Dalod M, Crozat K.GB11),ThermoFisher,#GRB05 Anti I-A/I-E (MHC-II) A700 (Clone M5/114.15.2),ThermoFisher eBioscience,#56-5321-82 Anti IL-12(p40/p70) APC (Clone C15.6),BD Biosciences,#554480 Anti IFNγ A700 (Clone XMG1.2),BD Biosciences,#557998 Anti

(20) Early activation of the cardiac CX3CL1/CX3CR1 axis delays β-adrenergic-induced heart failureScientific reportsSeptember 9, 2021Flamant M, Mougenot N, Balse E, Le Fèvre L, Atassi F, Gautier EL, Le Goff W, Keck M, Nadaud S, Combadière C, et al.

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8/31/2018Consistent MHCII Antibody for Flow CytometryDr. Amy StoneThis antibody was selected as part of a panel studying innate immune responses to RNA viruses. The goal of this work is to determine the ability of the innate immune system to function against viral ... Read Review

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MHC Class II (I-A/I-E) Antibody Alexa Fluor 700 from Thermo Fisher Scientific

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MHC Class II (I-A/I-E) Antibody Alexa Fluor 700 from Thermo Fisher Scientific

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