Fig 2: Characterization of murine BMMSCs. a, b Flow cytometric analysis of the surface markers showed positive expression of CD29, CD90, CD105, CD146, and Sca-1, and negative expression of hematopoietic markers CD11b, CD34, and CD45. c Representative image of colony formation of murine BMMSCs. d Representative image of Oil Red O staining of murine BMMSCs. e Representative image of ALP staining of murine BMMSCs. f Representative image of Alizarin red staining of murine BMMSCs. Scale bar, 500 µm

Fig 3: Expression of CD90 marker in pituispheres and MSC. Immunofluorescence images of PA02, PA03, and PA05 (A) MSC and (B) pituispheres stained for CD90 (green). Isotype controls were used as negative control. Cell nuclei were counterstained with DAPI (blue). Scale bar, 13 µm.

Fig 4: Identification of ADMSCs. (A) Flow cytometry detection of surface antigens on ADMSCs: after induction, the CD29+, CD40+, and CD90+ cells were the highest populations of stem cells. (B) Representative images of osteogenic differentiation of ADMSCs as detected by Alizarin Red S method; osteogenic ADMSCs were stained red. (C) representative images of adipogenic differentiation of ADMSCs as detected by Oil Red O method; adipogenic ADMSCs were stained red. Magnification: 400×.

Fig 5: Identifications of CPSCs. a) CPSCs exhibited spindle-like morphology, chondrogenesis, and osteogenesis. Scale bar = 100 µm. b) Flow cytometric analysis of CD29 and CD90, characteristic positive markers of CPSCs, and the negative marker CD45. c) Morphology of the colony after cells were cultured for 1, 3, 5, 7 days and the colonies were stained with crystal violet. Scale bars: 200 µm (day 1), 100 µm (day 3) and 500 µm (day 5/7).