Great CD90.1 Antibody from Thermo Fisher Scientific

Innate Immunity
DRFZ / Charité – Universitätsmedizin Berlin
Graduate Student

Overall

Quality of Results

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Company:

Thermo Fisher Scientific

Product Name:

CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), FITC

Catalog Number:

11-0900-81

Clone Number:

HIS51

The aim of our research is to check the different expression profiles, in terms of cytokines and surface markers, of in vitro differentiated CD4+ T cells. This antibody was used in order to distinguish between naive T cells derived from wild type mice and mutant mice (carrying the congenic marker Thy-1.1)

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD4+ murine T cells

Primary Incubation

15mins in 4°C

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Able to distinguish between the populations of interest. The antibody was good and specific. The resolution could be higher.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good, sensitive, high resolution

The Bad

Resolution can be improved

The Bottom Line

Would recommend it

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