Western blot was performed using Anti-MyD88 Polyclonal Antibody (Product # 14-6223-63) and a 35kDa band corresponding to MyD88 was observed in all cell lines and tissues tested. Whole cell extracts (30 µg lysate) of MOLT-4 (Lane 1), Jurkat (Lane 2), SK-BR-3 (Lane 3), HeLa (Lane 4), Mouse Spleen (Lane 5) and Mouse Lung (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).