Intracellular staining of 3-day Anti-Human CD3 and Anti-Human CD28 Functional Grade Purified (Product # 16-0039-81 and Product # 16-0289-81) stimulated human peripheral blood cells with Mouse IgG1 kappa Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human BATF PerCP-eFluor® 710 (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Viable lymphocytes, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis. Intracellular staining of unstimulated cells with Anti-Human BATF PerCP-eFluor® 710 is shown in the orange histogram.