JAWS II DC undergo maturation when cultured in SMG. (a) Unstimulated and stimulated JAWS II DC (2 × 105/ml) were cultured in Static (light gray) or SMG (dark gray) conditions for 72 h. JAWS II DC activated during Static or SMG culture were either incubated with (stimulated) or without (unstimulated) a cocktail of cytokines (IFN-γ, IL-4 and TNFα). Afterwards, the cells were collected and stained with antibodies for MHC class I and II, CD80, CD86, 4-1BBL and DC-SIGN and their corresponding isotype controls. Open histograms with broken lines represent isotype controls. (b) Graph represents the MFI of each of the surface molecules examined in (a) for unstimulated Static (light gray bars) and SMG JAWS II DC (black bars). (c) Culture supernatants, collected from unstimulated and stimulated JAWS II DC (2 × 105/ml) cultured as in (a), were assessed for IL-6 production by ELISA. One representation of two independent experiments with similar results is shown for (a,b). For (c), the data represents the mean + SD of quadruplicates of two independent experiments. In (b), *p-value ≤ 0.05 comparing the expression of surface molecules by SMG and Static JAWS II DC. In (c), *p-value ≤ 0.05 comparing the production of IL-6 by unstimulated and stimulated SMG to Static JAWS II DC.