C57Bl/6 splenocytes were cultured for 3 days with Concanavalin A then stained with Anti-Human/Mouse CD45R (B220) APC-eFluor® 780 (Product # 47-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.25 µg of Anti-Mouse CD166 (ALCAM) APC (right). Viable cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.