CD3 Antibody Functional Grade 16-0037-81 from Thermo Fisher Scientific

Product Specs
ItemCD3 Antibody Functional Grade
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number16-0037-81
Quantity50 µg
ApplicationsFlow Cytometry, Functional assay
Concentration1 mg/mL
Conjugate/TagFunctional Grade
FormatTBS with no preservative; pH 8.0
PurityAffinity chromatography
ReactivityHuman
TargetCD3
IsotypeIgG2a kappa
CloneOKT3
HostMouse
Storage Temperature4° C
Antibody TypePrimary
ClonalityMonoclonal
Storage4°C
NCBI Full Gene NameCD3 epsilon subunit of T-cell receptor complex, CD3 gamma subunit of T-cell receptor complex, CD247 molecule, CD3 delta subunit of T-cell receptor complex
NCBI Gene AliasesCD3epsilon, CD3-GAMMA, CD3Z, CD3-ZETA, CD3GAMMA, T3G, T3D, T3E, TCRE, CD3H, TCRZ, IMD18, IMD19, CD3DELTA, CD3Q, CD3-DELTA, T3Z, IMD25, IMD17, CD3ZETA

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Exosomes derived from AHR-overexpressing human umbilical cord mesenchymal stem cells attenuate H/R injury via AHR/NLRP3 pathwayCell CycleDecember 15, 2026Ying Qin, Dongyan Shen, Kequan Guo, Xu Meng, Xiaozheng Cui, Yongchao Li, Yixin JiaPA5-18337), FOXP3 Monoclonal Antibody (236A/E7, Cat. No. 53–4777-42), CD3 Monoclonal Antibody (OKT3, Cat. No. 16–0037-81), and CD8a Monoclonal Antibody (RPA-T8, Cat. No. 12–0088-80) were purchased from Thermo Fisher Scientific

(2) Establishment of Patient-Derived Organoids for Pediatric Cancer ResearchCancers (Basel)May 2, 2026Muhammad Younis, Tarlan Arjmandi, Mohammad Haque, Katherine McClain, Thussenthan Walter-Angelo, Franklin Back, Divya Gandra, Abigail Moore, Chandrika Behura, Vladimir S. Spiegelman, et al.CA, USA; #422302) human CD45 (Invitrogen; Carlsbad, CA, USA; Cat # 58-0459-42), CD3 (Invitrogen Cat # 16-0037-81), B220 (BioLegend Cat# 103206) and CD11b (BioLegend Cat # 101241), FACS stain buffer (BD Pharmingen;

(3) ROS-Responsive MnO2 nanozyme co-delivering cGAMP and TGF-β inhibitor synergistically activates STING signaling and remodels the immunosuppressive thyroid cancer microenvironmentJournal of NanobiotechnologyApril 11, 2026Guangming Liu, Rui LiThe purified CD8+ T cells were seeded into 24-well plates pre-coated with anti-CD3 antibody (2 µg/mL, 16-0037-81, eBioscience, USA) and cultured with anti-CD28 antibody (2 µg/mL, 16-0289-85, eBioscience, USA) and

(4) Immuno-Instructive 3D Tendon Biomimetic Scaffolds Functionalized with Amniotic Epithelial Stem Cell Secretome for Controlled Inflammation and Targeted Macrophage PolarizationInternational Journal of Molecular SciencesFebruary 20, 2026Mohammad El Khatib, Annunziata Mauro, Giuseppe Prencipe, Oriana Di Giacinto, Valeria Giovanna Festinese, Carola Agostinone, Maura Turriani, Paolo Berardinelli, Barbara Barboni, Valentina Russocells per well in a 96-well high-binding plate (Corning #9018) pre-coated with anti-CD3 (2 µg/mL, #16-0037-81) and anti-CD28 (2 µg/mL, #16-0289-81) antibodies (Invitrogen, Thermo Fisher Scientific). 4.9.1. Direct

(5) Up-regulation of an epithelial miRNA is associated with immune evasion in progressive bronchial premalignant lesionsCancer Immunology ResearchFebruary 11, 2026Boting Ning, Darren J. Chiu, Roxana M. Pfefferkorn, Erin Cullinane, Yohana Kefella, Erin Kane, Valerie Reyes-Ortiz, Gang Liu, Xiaohui Zhang, Hanqiao Liu, et al.(Fisher Scientific Cat#200–02-10UG) with the addition of anti-human CD3 (clone OKT3, Thermofisher Cat#16–0037-81, RRID: AB_468854, 1ug/mL in 2ml) and anti-human CD28 (clone CD28.2, Thermofisher Cat#16–0289-81, RRID:

(6) Upregulation of an Epithelial miRNA Is Associated with Immune Evasion in Progressive Bronchial Premalignant LesionsCancer Immunol ResFebruary 11, 2026Boting Ning, Darren J. Chiu, Roxana M. Pfefferkorn, Erin Cullinane, Yohana Kefella, Erin Kane, Valerie Reyes-Ortiz, Gang Liu, Xiaohui Zhang, Hanqiao Liu, et al.cat. #200-02-10UG) with the addition of anti–human CD3 (clone OKT3, Thermo Fisher Scientific, cat. #16-0037-81, RRID: AB_468854; 1 μg/mL in 2 mL) and anti–human CD28 (clone CD28.2, Thermo Fisher Scientific, cat.

(7) Engineered extracellular vesicles displaying bi-specific T-cell engagers for targeted therapy of B-cell malignanciesExp Hematol OncolFebruary 7, 2026Xiuxiu Yang, Qian Xu, Jue Wang, Shanwei Ye, Caroline Markmann, Shujia Zhang, Qian Zhang, Vijay G. Bhoj, Liang Huang, Zheng Zhangwith BiTE EVs for 24 h. GFP expression was measured by flow cytometry. OKT3 (1 µg/µL; Thermo Fisher, #16-0037-81) served as a positive control. In vitro cytotoxicity Luciferase-expressing target cells (Raji, CD19

(8) SLC6A14-mediated glutamine promotes SYTL4–CXCL8 axis activation to drive gemcitabine resistance and immune evasion in pancreatic cancerExperimental & Molecular MedicineDecember 25, 2025Hyeon Woong Kang, Ju Hyun Kim, Jae Woong Jeong, Sungsoon Fang, Won-Gun Yun, Hye-Sol Jung, Wooil Kwon, Jin-Young Jang, Hyo Jung Kim, Joon Seong Parksulfoxide and stored at −80 °C. T cell isolation and activation Anti-CD3 (Thermofisher, cat. no. 16-0037-81) was diluted in PBS and precoated in a six-well plate. T cells were isolated using the Pan T cell isolation

(9) SAA restricts T cell mediated anti-tumor immunity by limiting antigen presentation in lung cancerFront ImmunolDecember 19, 2025Mei Huang, Run Shi, Cong Xu, Yihan Zhang, Xiaoyue Du, Shaodi Wen, Chunbin Wang, Feng Jiang, Guoren Zhou, Xin Wang, et al.SinoBiological), TNF (10602-HNAE, SinoBiological), IL2 (11848-HNAH1-E, SinoBiological), CD3 antibody (16-0037-81, ThermoFisher), CD28 antibody (14-0289-82, ThermoFisher), SAA antibody (924903, R&D), rec. SAA1 (300-53,

(10) Multimodal analysis defines GNG4 as a distinguishing feature of germinal center-positioned CD4 T follicular helper cells in humansbioRxivDecember 13, 2025Sam Barnett Dubensky, Yutong Zhu, Molly Gallagher, Kingsley Gideon Kumashie, Tianyu Lu, Jonathan Tedesco, Nina De Luna, Katherine Premo, Yi Qi, Suzanna Rachimi, et al.TC-treated flat-bottom 96-well plate (Fisherbrand, FB012931) was coated with 50 μL anti-CD3 (Invitrogen, 16-0037-81) diluted to 2 μg/mL in PBS, then washed three times with PBS. 1e6 cells were stimulated per well in

(11) CD5 ablation enhances persistence and antitumor potency of engineered T cells by mitigating exhaustion and promoting cytotoxicityJournal for ImmunoTherapy of CancerNovember 29, 2025Jia Wu, Jiali Cheng, Li Zhu, Qiang Gao, Haolong Lin, Yuhao Zeng, Yong Li, Weini Li, Ning An, Liang Huang, et al.stimulated through TCR engagement using varying concentrations of CD3 monoclonal antibody (Thermo Fisher, 16-0037-81). EGFP expression levels were analyzed by flow cytometry at the specified time points. Gene expression analysis

(12) Safe immunosuppression-resistant pan-cancer immunotherapeutics by velcro-like density-dependent targeting of tumor-associated carbohydrate antigensCellNovember 26, 2025Zhou RW, Purohit PK, Kim JH, Lee SU, Burshteyn N, Tifrea D, Cordon A, Grigorian A, Newton BL, Edwards RA, et al.(236A/E7),Thermo Fisher,17-4777-42; RRID:AB_10804651 "Human CD3 (OKT3), Functional Grade",Thermo Fisher,16-0037-81; RRID:AB_468854 "Human CD28 (CD28.2), Functional Grade",Thermo Fisher,16-0289-81; RRID:AB_468926 Human

(13) Study on the in vitro and in vivo killing effects of PD-1 antibody-secreting c-Met-targeted CAR-T cells on esophageal cancer cell line ECA109Discov OncolOctober 21, 2025Shang Peng, Yanqiu Li, Yanjun Zhang, Jingting Min, Ran An, Nana Du, Haipeng Li, Xiangcheng Zhen, Fei Chu, Zhenghong Li(PBMCs) were isolated from healthy volunteers and activated with mouse anti-CD3/CD28 antibodies (Gibco#16-0037-81, 16-0289-81). Active T cells were infected with lentivirus at a multiplicity of infection (MOI) of 5

(14) Modulation of fibronectin extracellular matrix enhances anti-tumor efficacy of immune checkpoint blockadeCell Reports MedicineSeptember 16, 2025Kabir A. Khan, Maresa Caunt Mitzner, William Cruz-Munoz, Grant Li, Patricia Himmels, Ping Xu, Hung Yang Kuo, Raj Jesudason, Alvin Gogineni, Robby Weimer, et al.Pen/Strep, Glutamine, 10 ng/mL rh-IL2 (R&D cat no # 202-IL-010) and 50 ng/mL anti-human CD3 (eBioscience 16-0037-81). On day of assay activated CD8+ T-cells were incubated in 0.5 μM of Cell Tracker Green CMFDA Dye (Thermoscientific

(15) A CXCR4 partial agonist improves immunotherapy by targeting immunosuppressive neutrophils and cancer-driven granulopoiesisCancer CellAugust 11, 2025Jin Qian, Chenkai Ma, Quin T. Waterbury, Xiaofei Zhi, Christine S. Moon, Ruhong Tu, Hiroki Kobayashi, Feijing Wu, Biyun Zheng, Yi Zeng, et al.,BD Biosciences,"Cat# 553295, RRID:AB_394764" Purified anti-CD3 (OKT-3),Thermo Fisher Scientific,"Cat# 16-0037-81, RRID:AB_468854" Purified anti-CD28 (28.2),Thermo Fisher Scientific,"Cat# 16-0289-81, RRID:AB_468926" PE-Cy7

(16) Impact of 3D cell culture hydrogels derived from basement membrane extracts or nanofibrillar cellulose on CAR-T cell activationiScienceJuly 30, 2025Sonia Aristin Revilla, Alessandro Cutilli, Eugenia Cambiaso, Dedeke Rockx-Brouwer, Cynthia Lisanne Frederiks, Marc Falandt, Riccardo Levato, Onno Kranenburg, Caroline A. Lindemans, Paul James Coffer, et al.grade,eBioscience,Cat# 16-0281-81; RRID: AB_468924 Anti-Human CD3e functional grade,eBioscience,Cat# 16-0037-81; RRID: AB_468854 Anti-Human CD28 functional grade,eBioscience,Cat# 16-0289-85; RRID: AB_468926 Anti-Mouse

(17) Inhibition of FAK promotes pancreatic cancer immunotherapy by mediating CXCL10 secretion to enhance CD8+ T cell infiltrationOncoimmunologyJuly 29, 2025Yu-Chen Shi, Qingling An, Na Tang, Yong-Qiang Zhang, Shu-Qiao Xing, Fan Song, Xiao-Qiang Liand BXPC3-FAK-KD cells for 24 h, PBMCs pre-activated with CD3 (1 µg/ml)/CD28 (1 µg/ml) (Thermofisher, 16–0037–81/16–0289–81) antibodies for 24 h were added, followed by an additional 24 h co-culture period. Subsequently,

(18) Phosphoglycerate mutase regulates Treg differentiation through control of serine synthesis and one-carbon metabolismeLifeJuly 28, 2025Godfrey WH, Lee JJ, Shanmukha S, Cho K, Deng X, Ambati CSR, Putluri V, Mostafa Kamal AH, Kim PM, Putluri N, et al.RRID:AB_3212871",1:200 Antibody,"Anti-CD3 (OKT3), Functional Grade, eBioscience (Mouse monoclonal)",Thermo Fisher,"Cat # 16-0037-81, RRID:AB_468854",2 μg/ml Antibody,"Anti-CD28 (37.51), Functional Grade, eBioscience (Syrian hamster

(19) Inhibition of FAK promotes pancreatic cancer immunotherapy by mediating CXCL10 secretion to enhance CD8+ T cell infiltrationOncoimmunologyJuly 28, 2025Yu-Chen Shi, Qingling An, Na Tang, Yong-Qiang Zhang, Shu-Qiao Xing, Fan Song, Xiao-Qiang Liand BXPC3-FAK-KD cells for 24 h, PBMCs pre-activated with CD3 (1 µg/ml)/CD28 (1 µg/ml) (Thermofisher, 16–0037–81/16–0289–81) antibodies for 24 h were added, followed by an additional 24 h co-culture period. Subsequently,

(20) Altered immunometabolic response to fasting in humans living with obesityiScienceJuly 18, 2025Helena Neudorf, Roderick E. Sandilands, Spencer Ursel, Hillary Shaba, Darren Barg, Takeshi Tsusaka, María Dolores Moya-Garzón, Erica Vaz, Patricia Schimweg, Emily L. Goldberg, et al.Baseline and 48 h, a 48-well plate was incubated for 2 h at 37°C with CD3 (5 μg/ml, Invitrogen, Cat. No. 16-0037-81) to allow binding to the plate. Next, the CD3 liquid was removed, and 8 × 105 freshly isolated T cells

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CD3 Antibody Functional Grade from Thermo Fisher Scientific

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