Western blot was performed using Anti-N-WASP Polyclonal Antibody (Product # PA5-52198) and a ~60 kDa band corresponding to N-WASP was observed along with an uncharacterized band (*) at ~35 kDa across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of SK-BR-3 (Lane 1), SH-SY5Y (Lane 2), MCF7 (Lane 3), NIH/3T3 (Lane 4), PC-12 (Lane 5); and tissue extract Mouse Lung (Lane 6) and Mouse Cerebellum (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.04 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).