Anti-ROC1 RBX1 Antibody A00524 from BosterBio

Product Specs
ItemAnti-ROC1 RBX1 Antibody
CompanyBosterBio
Price $641.00Supplier Page View Company Product Page
Catalog NumberA00524
Quantity100 µl
ApplicationsELISA,IHC,WB
Format0.01% (w/v) Sodium Azide, Liquid (sterile filtered)
ReactivityHuman
HostRabbit
NCBI Gene AliasesBA554C12.1, RNF75, ROC1
ClonalityPolyclonal
PurityThis product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC1. Cross-reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11
ImmunogenThis antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 of Human ROC1 (C-terminal) coupled to KLH
Concentration85 mg/mL by Refractometry
StorageStore vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening
IsotypeAntiserum
Gene NameRBX1
Antibody TypePrimary Antibodies
TargetTranscription factor GATA-4
Molecular Weight44565 MW

Specifications/Features

Application Notes: This antibody reacts with human ROC1 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation of ubiquitin ligase activity will also occur. A 12.2 kDa band corresponding to human ROC1 is detected. Most cell lines expressing ROC1 can be used as a positive control. Researchers should determine optimal titers for other applications.

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