Fig 1: Effects of Serpinb1a on osteoclast formation. RAW 264.7 cells transiently expressing Serpinb1a or an empty vector were cultured with aMEM, 10% FBS and 50 ng/ml RANKL for 6 days. (A) The number of TRAP-positive MNCs was counted. (B) The number of CTR-positive MNCs was counted. Data shows the representative images (A, B). Scale bar indicates 50 µm (A, B). Arrowheads indicates TRAP- or CTR-positive MNCs (A, B). (C) Total RNA was extracted, and qRT-PCR for NFATc1, TRAP, cathepsin K (Ctsk), Serpinb1a or GAPDH was performed. Data are expressed relative to GAPDH mRNA. Data represent the mean ± SEM (n = 4 for the empty vector or Serpinb1a without RANKL group and 8 for the empty vector or Serpinb1a with RANKL group). **p < 0.01, *p < 0.05 by the Tukey-Kramer test. ##p < 0.01 by the Mann-Whitney U test.