PHD-2/Egln1 (D31E11) Rabbit mAb 4835S from Cell Signaling Technology

Product Specs
ItemPHD-2/Egln1 (D31E11) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number4835S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Mk
TargetPHD-2 (Egln1)
IsotypeIgG
HostRabbit
NCBI Gene AliasesC1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6
NCBI Full Gene Nameegl-9 family hypoxia inducible factor 1
CloneD31E11
Molecular Weight50
ClonalityMonoclonal
Research AreaDevelopmental Biology

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Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Distinct HIF1α and HIF2α functions control skeletal muscle metabolism and erythropoiesisJournal of Clinical InvestigationApril 15, 2026Junhyeong Lee, Merc Emil Matienzo, Sangyi Lim, Edzel Evallo, Yeongsin Kim, Sujin Jang, Keon Kim, Chang Hyeon Choi, Youn Ho Han, Chang-Min Lee, et al.ABclonal), OXPHOS (ab110413; Abcam), OPA1 (90471; Cell Signaling Technology), PHD1 (ab113077; Abcam), PHD2 (4835; Cell Signaling Technology), PHD3 (NB100-303; Novus Biologicals), and V5 (13202; Cell Signaling Technology)

(2) Distinct HIF1α and HIF2α functions control skeletal muscle metabolism and erythropoiesisJ Clin InvestFebruary 17, 2026Junhyeong Lee, Merc Emil Matienzo, Sangyi Lim, Edzel Evallo, Yeongsin Kim, Sujin Jang, Keon Kim, Chang Hyeon Choi, Youn Ho Han, Chang-Min Lee, et al.ABclonal), OXPHOS (ab110413; Abcam), OPA1 (90471; Cell Signaling Technology), PHD1 (ab113077; Abcam), PHD2 (4835; Cell Signaling Technology), PHD3 (NB100-303; Novus Biologicals), and V5 (13202; Cell Signaling Technology)

(3) SKA3-mediated hypoxia tolerance and metabolic reprogramming promote liver metastasis in lung adenocarcinomaCell Death & DiseaseNovember 26, 2025Zedong Sun, Minmin Zhou, Ningdan Song, Can Wang, Mingrui Liu, Simian He, Feifan Ji, Jiayu Chen, Lin Xu, Xiufen Zheng, et al.in IP lysis buffer and protease inhibitors. For immunoprecipitation, antibodies against PHD2 (1:200; 4835, CST), SKA3 (1:200; 186003, Abcam) were added to the lysates and incubated overnight at 4 °C, with rabbit

(4) HIF-1 regulates mitochondrial function in bone marrow-derived macrophages but not in tissue-resident alveolar macrophagesScientific reportsApril 4, 2025Woods PS, Cetin-Atalay R, Meliton AY, Sun KA, Shamaa OR, Shin KWD, Tian Y, Haugen B, Hamanaka RB, Mutlu GM.rabbit anti-LDHA (Cell Signaling, Cat# 20125, 1:1000), rabbit anti-PHD2/Egln1 (Cell Signaling, Cat# 4835, 1:1000), rabbit anti-MCT4 (Proteintech, Cat# 22787-1-AP), rabbit anti-Lamin B1 (Proteintech, Cat# 12987-1-AP,

(5) Ginsenoside CK targets PHD2 to prevent platelet adhesion and enhance blood circulation by modifying the three-dimensional arrangement of collagenActa Pharmaceutica Sinica BMarch 1, 2025Chuanjing Cheng, Kaixin Liu, Jinling Zhang, Yanqi Han, Tiejun Zhang, Yuanyuan Hou, Gang Baiaspirin (ASP, A8830) were supplied by Beijing Solarbio Co. (Beijing, China). Antibodies targeting PHD2 (4835S), HIF-1α (48085S), VEGF (9698S), collagen I (72026S), and GAPDH (5174S) were obtained from Cell Signaling Technology

(6) Crosslinking-mediated Interactome Analysis Identified PHD2-HIF1α Interaction Hotspots and the Role of PHD2 in Regulating Protein NeddylationbioRxivDecember 25, 2024Haiping Ouyang, Cindy Y. How, Xiaorong Wang, Clinton Yu, Ang Luo, Lan Huang, Yue Chen(Montgomery, TX). Anti-NEDD8(2754T), anti-CUL1(4995S), anti-CUL3 (2759S), anti-CUL4A (CSIG-2699T), anti-PHD2 (4835), anti-NRF2 (12721T) antibodies and HRP-linked secondary antibody (7076 and 7074) were from Cell Signaling Technology

(7) Branched-chain α-ketoacids aerobically activate HIF1α signalling in vascular cellsNature MetabolismOctober 29, 2024Wusheng Xiao, Nishith Shrimali, Niv Vigder, William M. Oldham, Clary B. Clish, Huamei He, Samantha J. Wong, Bradley M. Wertheim, Elena Arons, Marcia C. Haigis, et al.BD Biosciences; human BCAT2 (cat # 9432), LDHA (cat # 2012), MDH2 (cat # 11908), PHD2 (cat # 3293 and 4835), pVHL (cat # 68547), hydroxylated HIF1α-Pro564 (cat # 3434), VIM (cat # 5741), human and rat phospho-BCKDH-E1α

(8) Salidroside rescues hypoxic cardiomyocytes by regulating the EGLN1/HIF‑1α pathwayBiomedical ReportsOctober 2, 2024Wenmao Zhang, Ziling Liao, Chengfeng Xu, Xinping Linpurchased from MilliporeSigma. Tween-20 was purchased from Amresco Co., Ltd. Anti-EGLN1 (cat. no. #4835s) was purchased from Cell Signaling Technology, Inc. Anti-HIF-1α (cat. no. NB100-105) was purchased from

(9) Genetic modifications of EGLN1 reactivate HbF production in β0-thalassemia/HbEHeliyonSeptember 30, 2024Varit Jan-ngam, Siriraj Boontha, Alisa Tubsuwan, Somsakul Pop Wongpalee, Kanda Fanhchaksai, Adisak Tantiworawit, Pimlak Charoenkwan, Pinyaphat Khamphikhamskim milk in PBS-T buffer and was incubated with primary antibodies including rabbit anti-human PHD2 (#4835; Cell Signaling, Danvers, MA), rabbit anti-human GATA1 (#4591; Cell Signaling), mouse anti-human BCL11A

(10) An active peptide from yak inhibits hypoxia-induced lung injury via suppressing VEGF/MAPK/inflammatory signalingRedox BiologySeptember 1, 2024Feiyan Yang, Zeyu He, Zhongxing Chu, Wen Li, Guangfan Qu, Han Lu, Yiping Tang, Shuguo Sun, Zhang Luo, Feijun Luodilution, 3498, Cell Signaling), Bax (1:5000 dilution, 5023, Cell Signaling), PHD-2 (1:5000 dilution, 4835, Cell Signaling), Glut4 (1:5000 dilution, 2213, Cell Signaling), p38 (1:5000 dilution, 9212, Cell Signaling),

(11) TFE3 fusions direct an oncogenic transcriptional program that drives OXPHOS and unveils vulnerabilities in translocation renal cell carcinomabioRxivAugust 14, 2024Jiao Li, Kaimeng Huang, Fiona McBride, Ananthan Sadagopan, Daniel. S Gallant, Meha Thakur, Prateek Khanna, Bingchen Li, Maolin Ge, Cary N. Weiss, et al.AB_10624872), HIF1A (Fisher Scientific, Cat#: BDB610959; RRID: N/A), EGLN1 (Cell Signaling Tech, Cat#: 4835S; RRID: AB_10561316), VHL (Cell Signaling Tech, Cat#: 68547; RRID: AB_2716279), ACTIN (Cell Signaling

(12) Prolyl hydroxylase domain enzyme PHD2 inhibits proliferation and metabolism in non-small cell lung cancer cells in HIF-dependent and HIF-independent mannersFrontiers in OncologyJune 27, 2024Hongyan Deng, Zixuan Wang, Chunchun Zhu, Zhu Chenassay Western blot analysis was performed as described previously (32). Antibodies comprising anti-PHD2 (#4835, Cell Signaling Technology), anti-HIF1α (#36169, Cell Signaling Technology), and anti-GAPDH antibody

(13) Modified Zhenwu Tang delays chronic renal failure progression by modulating oxidative stress and hypoxic responses in renal proximal tubular epithelial cellsHeliyonMay 30, 2024Zhang YY, Jin PP, Guo DZ, Bian D.levels of SOD1, MDA, HIF-1α, PHD2, and α-SMA in renal tissue, as well as HIF-1α and PHD2 (CST, 36169, 4835) in cells of each experimental group. Proteins from rat renal tissue and monolayer adherent cells were

(14) LINC02774 inhibits glycolysis in glioma to destabilize HIF-1α dependent on transcription factor RP58MedComm (2020)October 1, 2023Chen Y, Liu Y, Xiong J, Ouyang L, Tang M, Mao C, Li L, Xiao D, Liu S, Yang Z, et al.#NB100‐139, 1:1000), GLUT1 (CST, #12939S, 1:1000), RP58 (Proteintech, #12714‐1‐AP, 1:1000), PHD2 (CST, #4835S, 1:1000), VEGF (Abclonal, #A12303, 1:500), N‐cadherin (CST, #13116, 1:1000), HK2 (Proteintech, #22029‐1‐AP,

(15) Nicotinamide Antagonizes Lipopolysaccharide-Induced Hypoxic Cell Signals in Human MacrophagesJournal of immunology (Baltimore, Md. : 1950)July 15, 2023Curran CS, Dougherty EJ, Cui X, Li Y, Jeakle M, Gamble T, Demirkale CY, Torabi-Parizi P.Cell Signaling, 1:1000), NAMPT (MAB4044, R&D systems, 1 μg/mL), p65 (8242, Cell Signaling, 1:1000), (PHD2 (4835S, Cell Signaling, 1:3000), PSMF1 (Sigma HPA041300, Sigma, 0.4ug/ml), SART1 (ab8858, Abcam, 1μg/ml), SIRT1(s47)

(16) Collagen-Derived Dipeptide Pro-Hyp Enhanced ATDC5 Chondrocyte Differentiation under Hypoxic ConditionsMoleculesJune 9, 2023Yoshifumi Kimira, Takahiro Sato, Mayu Sakamoto, Yoshihiro Osawa, Hiroshi Manorabbit anti-Sox9 (catalog #36169; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Runx2 (4835; Cell Signaling Technology, Danvers, MA, USA), or rabbit anti-β-actin (3700; Cell Signaling Technology)

(17) HIF-1α accumulation in response to transient hypoglycemia may worsen diabetic eye diseaseCell reportsJanuary 31, 2023Guo C, Deshpande M, Niu Y, Kachwala I, Flores-Bellver M, Megarity H, Nuse T, Babapoor-Farrokhran S, Ramada M, Sanchez J, et al.anti-HIF-1a,Gene Tex,Cat# GTX127309; RRID:AB_2616089 Rabbit monoclonal anti-PHD2,Cell Signaling,Cat# 4835; RRID: N/A Mouse monoclonal anti-GAPDH,Sigma,Cat# G8795; RRID:AB_1078991 Rabbit monoclonal anti-VHL,Cell Signaling

(18) Identification of a Novel Angiogenesis Signalling circSCRG1/miR-1268b/NR4A1 Pathway in Atherosclerosis and the Regulatory Effects of TMP-PF In VitroMoleculesJanuary 28, 2023Rong Yuan, Qiqi Xin, Xiaochang Ma, Meng Yu, Yu Miao, Keji Chen, Weihong CongAb110776, Abcam, Cambridge, UK), RUNX1 (1:1000, Ab35962, Abcam, Cambridge, UK), EGLN1/PHD2 (1:1000, 4835s, CST, Boston, MA, USA), beta actin (1:1000, TA-09, ZSGB-BIO, Beijing, China) and GAPDH (1:1000, 5174,

(19) Adipocyte-derived lactate is a signalling metabolite that potentiates adipose macrophage inflammation via targeting PHD2Nature communicationsSeptember 5, 2022Feng T, Zhao X, Gu P, Yang W, Wang C, Guo Q, Long Q, Liu Q, Cheng Y, Li J, et al.Western Blotting Substrates (Bio-Rad). Antibodies used are: PDH2 (1:1000, Cell Signaling Technology, #4835 S, D31E11), iNOS (1:1000, Thermo Fisher Scientific, #PA3-030A), HIF-1α (1:1000, Proteintech, #20960-1-AP),

(20) HIF-1α induces glycolytic reprograming in tissue-resident alveolar macrophages to promote cell survival during acute lung injuryeLifeJuly 13, 2022Woods PS, Kimmig LM, Sun KA, Meliton AY, Shamaa OR, Tian Y, Cetin-Atalay R, Sharp WW, Hamanaka RB, Mutlu GM.rabbit anti-LDHA (Cell Signaling, Cat# 2012, 1:1000), rabbit anti-PHD2/Egln1 (Cell Signaling, Cat# 4835, 1:1000), rabbit anti-Egln3/PHD3 (Novus Biologicals, Cat# NB100-303, 1:1000), mouse anti-IL1β (Cell Signaling

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PHD-2/Egln1 (D31E11) Rabbit mAb

PHD-2/Egln1 (D31E11) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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PHD-2/Egln1 (D31E11) Rabbit mAb from Cell Signaling Technology

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