APC anti-mouse TCR beta chain 109212 from BioLegend

Product Specs
ItemAPC anti-mouse TCR beta chain
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number109212
Quantity100 µg
ApplicationsFlow Cytometry (FC)
Conjugate/TagAPC
IsotypeIgG
CloneH57-597
HostArmenian Hamster
ReactivityMouse
Antibody TypePrimary
NCBI Gene AliasesTCRbeta, Tib
NCBI Full Gene NameT cell receptor beta chain
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenAffinity purified TCR from mouse DO-11.10 cells
ClonalityMonoclonal
TargetTCR beta chain

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) CD163+ macrophages monitor enhanced permeability at the blood-dorsal root ganglion barrierJ Exp MedFebruary 5, 2024Harald Lund, Matthew A. Hunt, Zerina Kurtović, Katalin Sandor, Paul B. Kägy, Noah Fereydouni, Anais Julien, Christian Göritz, Elisa Vazquez-Liebanas, Maarja Andaloussi Mäe, et al.C068C2, 141716; Biolegend), CD11b-PEFire640 (clone M1/70, 101279; Biolegend) TCRb-APC (clone H57-597, 109212; Biolegend), MHCII-A700 (clone M5/114.15.2, 107622; Biolegend), XCR1-APC/Cy7 (clone ZET, 148223; Biolegend),

(2) Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysisSTAR ProtocolsDecember 1, 2023Enitome E. Bafor, Toni Martin, Jeffrey Carrell, Megan Karwan, Adrienne E. Kimmel, Uyi.G. Omogiade, Michael Sanford, Howard A. Young, Julio C. Valencia(1:100),BioLegend,Cat# 100706; RRID: AB_312745 Anti-mouse NK1.1 Brilliant Violet 711 (1:50),BioLegend,Cat# 109212; RRID: AB_313435 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant

(3) ICAM-1 mediated cell-cell adhesion exerts dual roles on human B cell differentiation and IgG productioniScienceNovember 22, 2023Shuai Liu, Zhi-cui Liu, Mei-yu Zhang, Shu-jun Wang, Meng Pan, Ping Ji, Cheng Zhu, Ping Lin, Ying WangAB_10900254" Anti-human-CD54-PE,BioLegend,"Cat# 353106, RRID: AB_10897647" Anti-human-αβTCR-APC,BioLegend,"Cat# 109212, RRID: AB_313435" Anti-human-HLA-DR-APC/Cy7,BD Biosciences,"Cat# 561358, RRID: AB_10611876" Anti-human-PD-1-BV650,BioLegend,"Cat#

(4) Reprogramming Cancer Cells to Antigen-presenting CellsBIO-PROTOCOLNovember 20, 2023Alexandra G. Ferreira, Olga Zimmermannova, Ilia Kurochkin, Ervin Ascic, Fritiof Åkerström, Carlos-Filipe Pereira25-0042-82)Anti-mouse TCR b chain Monoclonal Antibody (clone: H57-597), Allophycocyanin (BioLegend, catalog number: 109212)Anti-mouse/human CD44 Monoclonal Antibody (clone: IM7), Allophycocyanin-Cyanine7 (BioLegend, catalog

(5) Primary Mouse Invariant Natural Killer T (iNKT) Cell Purification and TransductionBIO-PROTOCOLJuly 5, 2023Gloria Delfanti, Paolo Dellabona, Giulia Casoraticlone: AF6-120.1 (BioLegend, catalog number: 114406)TCRβ-APC, clone: H57-597 (BioLegend, catalog number: 109212)Vβ5.2 PE/Cy7, clone MR9-4 (BioLegend, catalog number: 139508)Mouse PBS57-CD1d-tetramer, provided by

(6) The Immunosuppressive Potential of Cholesterol Sulfate Through T Cell Microvilli DisruptionImmune NetworkJune 15, 2023Jeong-Su Park, Ik-Joo Chung, Hye-Ran Kim, Chang-Duk Junwere purchased from Cell Signaling Technology (Danvers, MA, USA). Fluorescence-conjugated anti-TCRβ (109212), CD62L (104412), CD45 (368512), CD3ζ (152304), CD44 (103007), and ZAP70 (313404) were purchased from

(7) Gata3 is detrimental to regulatory T cell function in autoimmune diabetesbioRxiv : the preprint server for biologyMarch 23, 2023Kiaf B, Bode K, Schuster C, Kissler S.antibodies against CD45 PE-Cy 7 (clone: 30-F11, cat # 103114: Biolegend), TCRβ APC (clone: H57–597, cat # 109212: Biolegend) and with the viability dye Propidium Iodide (PI, cat # R37169; Thermo Fisher Scientific).

(8) Identification of T Cell Receptors Targeting a Neoantigen Derived from Recurrently Mutated FGFR3CancersFebruary 6, 2023Tate T, Matsumoto S, Nemoto K, Leisegang M, Nagayama S, Obama K, Nakamura Y, Kiyotani K.BioLegend (San Diego, CA, USA). We also obtained an anti-mouse TCRβ chain-APC antibody (clone H57-597, cat# 109212) from BioLegend (San Diego, CA, USA). We purchased peptide-HLA tetramers labeled with PE from MBL (Tokyo,

(9) Construction of a T cell receptor signaling range for spontaneous development of autoimmune diseaseJournal of Experimental MedicineFebruary 6, 2023Atsushi Tanaka, Shinji Maeda, Takashi Nomura, Mara Anais Llamas-Covarrubias, Satoshi Tanaka, Lin Jin, Ee Lyn Lim, Hiromasa Morikawa, Yohko Kitagawa, Shuji Akizuki, et al.(B21.5)-PE (12-5805-81; eBioscience), anti-Vβ11 (RR3-15)-PE (553198; BD), anti-TCR-β (H57-597)-APC (109212; Biolegend), anti-DO11.10 TCR (KJ1.26)-APC (17-5808-80; eBioscience), anti-IL-6 (MP5-20F3)-PE (12-7061-71;

(10) CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathologyNature immunologyNovember 1, 2022Knizkova D, Pribikova M, Draberova H, Semberova T, Trivic T, Synackova A, Ujevic A, Stefanovic J, Drobek A, Huranova M, et al.PerCP-Cy5.5 (405710), KLRG1 BV510 (138421), Ly6C PE-Cy7 (128018), Ly6G AF647 (127610), TCRβ PerCP (109212), MCP-1/CCL2 PE (505903), B220 AF700 (103232), TCRβ PE (109208), CD45.1 BV650 (110735), Flag APC (637307)

(11) Covalent TCR-peptide-MHC interactions induce T cell activation and redirect T cell fate in the thymusNature CommunicationsAugust 23, 2022Christopher Szeto, Pirooz Zareie, Rushika C. Wirasinha, Justin B. Zhang, Andrea T. Nguyen, Alan Riboldi-Tunnicliffe, Nicole L. La Gruta, Stephanie Gras, Stephen R. DaleypMHC tetramers for 1 h at RT. Cells were washed, incubated with APC anti-TCRβ (BioLegend, Cat. no. 109212) and LIVE/DEAD™ Fixable Aqua Dead Cell Stain (Thermofisher, Waltham, MA, Cat. no. L34957) for 30 min,

(12) Mobilization-based chemotherapy-free engraftment of gene-edited human hematopoietic stem cellsCellJune 23, 2022Omer-Javed A, Pedrazzani G, Albano L, Ghaus S, Latroche C, Manzi M, Ferrari S, Fiumara M, Jacob A, Vavassori V, et al.RRID:AB_394657 Anti-mouse PNA,Vector Laboratories,Cat# FL-1071; RRID:AB_2315097 Anti-mouse TCRbeta,BioLegend,Cat# 109211; RRID:AB_313434 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant

(13) Correlation between circulating innate lymphoid cell precursors and thymic functioniScienceFebruary 18, 2022Sandra Bajana, Aneta Pankow, Kaili Liu, Martyna Michniowska, Joseph F. Urban, Wei R. Chen, Xiao-Hong Sun118129, clone GL3, RRID:AB_2563356" Armenian hamster anti-mouse TCR beta chain APC,Biolegend,"Cat# 109212, clone H57-597, RRID:AB_313435" Armenian hamster anti-mouse TCR beta chain APC/Fire750,Biolegend,"Cat#

(14) DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosusiScienceJanuary 21, 2022Shiozawa S, Tsumiyama K, Miyazaki Y, Uto K, Sakurai K, Nakashima T, Matsuyama H, Doi A, Tarui M, Izumikawa M, et al.AB_15955573 Anti-Thy1.2-APC Ab,Biolegend,Cat#105311 RRID; AB_313182 Anti-TCRβ-APC Ab,Biolegend,Cat#109211 RRID; AB_313434 Anti-TLR7 Ab,BD Biosciences,Cat#565557 RRID; AB_2739295 Anti-Vβ8 TCR-PE Ab,BD Biosciences,Cat#553862

(15) HLA-A(∗)02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronavirusesCell reportsDecember 28, 2021Nesterenko PA, McLaughlin J, Tsai BL, Burton Sojo G, Cheng D, Zhao D, Mao Z, Bangayan NJ, Obusan MB, Su Y, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, NGFR-PECy7,Biolegend,"CAT# 345110, RRID:AB_11203542" murine TCRβ-APC,Biolegend,"CAT# 109212, RRID:AB_313435" CD3-APCCy7,Thermo Fisher,"CAT# 47-0036-42, RRID:AB_10717514" CD8a-PE,Thermo Fisher,"CAT#

(16) Parity-induced changes to mammary epithelial cells control NKT cell expansion and mammary oncogenesisCell ReportsDecember 7, 2021Amritha Varshini Hanasoge Hanasoge Somasundara et al.conjugated anti-TCR γ/δ (BioLegend Cat# 118108, RRID:AB_313832), APC conjugated anti-TCR β (BioLegend Cat# 109212, RRID:AB_313435), APC conjugated anti-H-2Kb (BioLegend Cat# 116517, RRID:AB_10568693), Pacific Blue

(17) Discovery of first-in-class inhibitors of ASH1L histone methyltransferase with anti-leukemic activityNature CommunicationsDecember 1, 2021David S. Rogawski et al.anti-CD11b (#101208, M1/70), Gr-1 (#108433, RB6-8C5), B220 (#103224, RA3-6B2), CD19 (#115520, 6D5), CD3 (#109211, H57-597), Zombie Aqua-dead cells (#423101) at 1:50 dilution. The analysis of CBC and other blood parameters

(18) Empirical identification and validation of tumor-targeting T cell receptors from circulation using autologous pancreatic tumor organoidsJournal for immunotherapy of cancerNovember 1, 2021Meng Q, Xie S, Gray GK, Dezfulian MH, Li W, Huang L, Akshinthala D, Ferrer E, Conahan C, Perea Del Pino S, et al.selected using puromycin (1 µg/mL). TCR-positive cells were sorted by FACS using mTCR antibody (Biolegend, 109212). SKW-3 cells were also transduced with human CD8 αβ and selected using blasticidin (final 10 µg/mL)

(19) Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapyCell reports. MedicineAugust 17, 2021Müller TR, Jarosch S, Hammel M, Leube J, Grassmann S, Bernard B, Effenberger M, Andrä I, Chaudhry MZ, Käuferle T, et al.#737657), CD8 PE (Invitrogen, reference #MHCD0804), anti-murine TCR β-chain APC (BioLegend, reference #109212), anti-murine TCR β-chain APC/Fire750 (BioLegend, reference #109246), CD62L FITC (Biolegend, reference

(20) Intrinsic Immunogenicity of Small Cell Lung Carcinoma Revealed by Its Cellular PlasticityCancer DiscoveryAugust 1, 2021Navin R. Mahadevan et al.After expansion, selected cells were sorted to >98% purity using an anti-mouse pan-TCR-β antibody (Cat# 109211, clone H57–597, Biolegend, San Diego, CA) expression (BD FACSMelody). Tumor cell-T cell cocultures were

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1/29/2020APC Anti-Mouse TCR Beta Chain (BioLegend) Detected TCR Expression in Isolated Mouse Splenocytes Anonymous ReviewerThe antibody can detect TCR beta on cell surface using flow cytometry.
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8/21/2019Review of Anti-Mouse TCRb (BioLegend) Sreya BagchiGood antibody for staining mouse TCRb+ cells.
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5/2/2017Anti-TCRb Identifies T Cells In The Lymph Node Very Well Robert YuanWe wanted to examine the lymph nodes in a diseased vs healthy state, and T cells were one cell of interest, leading us to use the antibody.

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APC anti-mouse TCR beta chain from BioLegend

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APC anti-mouse TCR beta chain from BioLegend

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