Fig 2: Targets of ES-62 in the BM and gut.(A) Data are presented as the mean ± SEM values for 3 individual mice (coloured symbols) in Naïve (days 0 and 36) and CIA-PBS groups (time points as indicated) where significant differences are indicated by **p<0.01 for the levels of Lin-Sca-1+c-kit+ (LSK) HSC (as % Lin- cells [frequency of precursor (FoP)]) over the phases of initiation (≤14 days), preclinical (day 21) and established joint pathology (≥ 28 days). Data are from the single experiment described in Figure 2. (B-D) Data are presented as the values for individual mice (coloured symbols, bar represents median of group) in each cohort and where microbiome depletion is denoted as “ABX” and square symbols. Significant differences are indicated by *p<0.05, ***p<0.001 and ****p<0.0001 for Lin-Sca-1+c-kit+ (LSK) HSCs (as absolute numbers (B) or (C) % Lin- cells [frequency of precursor (FoP)] and data are pooled from 2 experiments). BM CXCL12 mRNA levels are from the single experiment (of two) described in Figures 3, 4 (D). (E, F) Data are presented as the values of RANKL expression (MFI) for 3 individual mice (coloured symbols) RANKL+CD45- (E) and RANKL+B lineage (F) cells. Significant differences are indicated by *p<0.05 for RANKL expression by B220+ cells in the BM between the CIA-PBS and CIA-ES-62 groups. Levels of terminal fucosylation (as evidenced by Image J imaging of intensity of UEA-1 staining, see representative images of colon tissue [G]) are presented for ileum and colon tissue (G-I). Goblet cell numbers in ileum and colon tissue (J, K) were enumerated from PAS-stained sections and data are presented as the mean values for individual mice (coloured symbols, bar represents median of group). IL-1β mRNA expression in colon tissue (L) was determined by qRT-PCR and the data presented as the mean Rq values for individual mice (coloured symbols, bar represents median of group) in each cohort and where microbiome depletion is denoted as “ABX” and square symbols. Significant differences in panels (H-L) are indicated by *p<0.05 and ***p<0.001.