Human/Mouse ADAMTS1 Antibody AF5867 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman/Mouse ADAMTS1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF5867
NCBI Gene AliasesADAMTS, ADAMTS-1, METH-1, METH1, ADAM-TS1, C3-C5
IsotypeIgG
Conjugate/TagUnconjugated
TargetADAMTS1
HostSheep
ReactivityHuman,Mouse
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot
Quantity100 ug (also 25 ug)
NCBI Full Gene Namea disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1, ADAM metallopeptidase with thrombospondin type 1 motif 1
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenChinese hamster ovary cell line CHO-derived recombinant mouse ADAMTS1, Phe254-Cys725, Accession # P97857
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

What types of cancer has the ADAMTS1 antibody been shown to be relevant for in research studies?

The ADAMTS1 antibody has been implicated in studies related to oral squamous cell carcinoma, where it was found to correlate with invasive abilities and poor prognoses in patients. Additionally, it has been associated with uveal melanoma and glioblastoma, where it plays a role in endothelial-like properties and tumor progression.

How has the ADAMTS1 antibody been utilized in experimental setups according to the literature?

The ADAMTS1 antibody has been used in various applications including Western blotting and immunohistochemistry to assess protein expression levels in different cancer models. For instance, it was employed to analyze ADAMTS1 levels in tumor xenografts and cell lines, demonstrating its role in tumor invasiveness and stemness properties.

Are there any specific findings regarding the mechanism of action of ADAMTS1 in cancer progression?

Yes, studies have shown that ADAMTS1 can activate the epidermal growth factor receptor (EGFR) signaling pathway, which is crucial for promoting anoikis resistance and invasion in renal cell carcinoma. This suggests that ADAMTS1 may facilitate tumor progression through its enzymatic activity and interaction with other signaling molecules.

What is the significance of using the ADAMTS1 antibody in the context of glioblastoma research?

In glioblastoma research, the ADAMTS1 antibody has been shown to support the endothelial plasticity of glioblastoma cells, indicating its role in the formation of endothelial-like networks. This plasticity is significant for tumor growth and resistance to therapies, highlighting the potential of ADAMTS1 as a therapeutic target in glioma treatment.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The oncogenic ADAMTS1–VCAN–EGFR cyclic axis drives anoikis resistance and invasion in renal cell carcinomaCellular & Molecular Biology LettersSeptember 27, 2024Yu-Ching Wen, Yung-Wei Lin, Kuo-Hao Ho, Yi-Chieh Yang, Feng-Ru Lai, Chih-Ying Chu, Ji-Qing Chen, Wei-Jiunn Lee, Ming-Hsien ChienAbcam, Cambridge, MA); GAPDH (60,004–1-Ig), α-tubulin (66,031–1-Ig; Proteintech, Chicago, IL); ADAMTS1 (AF5867; R&D Systems); and c-Jun (GTX101135; GeneTex, Irvine, CA). Data collection from bioinformatics analyses Messenger

(2) Cyclic increase in the ADAMTS1-L1CAM-EGFR axis promotes the EMT and cervical lymph node metastasis of oral squamous cell carcinomaCell Death & DiseaseJanuary 23, 2024Ming-Hsien Chien, Yi-Chieh Yang, Kuo-Hao Ho, Yi-Fang Ding, Li-Hsin Chen, Wen-Kuan Chiu, Ji-Qing Chen, Min-Che Tung, Michael Hsiao, Wei-Jiunn Leewere obtained from Cell Signaling Technology (Danvers, MA, USA). An antibody specific for ADAMTS1 (AF5867) was purchased from R&D Systems, while those for L1CAM (sc514360) and TGF-β (sc130348) were from Santa Cruz Biotechnology

(3) Tau Protein Modulates Perineuronal Extracellular Matrix Expression in the TauP301L-acan Mouse ModelBiomoleculesMarch 26, 2022Schmidt S, Holzer M, Arendt T, Sonntag M, Morawski M.compared to TauP301LHET-acanWT mice (p = 0.001, n = 8, t-test). (B–D) Western blot analysis of ADAMTS1 (B, AF5867 (R&D Systems), at ~70 kDA), MMP3 (C, clone EP1186Y (Abcam), at ~50 kDA) and TIMP3 (D, anti-TIMP3 (Proteintech),

(4) ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairmentEuropean journal of human genetics : EJHGJanuary 1, 2022Bharadwaj T, Schrauwen I, Rehman S, Liaqat K, Acharya A, Giese APJ, Nouel-Saied LM, Nasir A, Everard JL, Pollock LM, et al.

(5) MYH9 facilitates autoregulation of adipose tissue depot developmentJCI insightMay 10, 2021Cheung SY, Sayeed M, Nakuluri K, Li L, Feldman BJ.membranes. Membranes were probed with primary antibodies (ADAMTS1; 1:1000; R&D Systems, Bio-Techne; catalog AF5867), MYH9 (1:1000; Cell Signaling Technology, catalog 3403), β-actin (1:2000; GenScript, catalog A007002)

(6) ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma ProgressionBiomoleculesDecember 31, 2020Serrano-Garrido O, Peris-Torres C, Redondo-García S, Asenjo HG, Plaza-Calonge MDC, Fernandez-Luna JL, Rodríguez-Manzaneque JC.Then, they were blocked with 5% low-fat milk and incubated with a sheep anti-human ADAMTS1 antibody (AF5867, R&D Systems, Minneapolis, MN, USA). After incubation with the appropriate peroxidase-conjugated secondary

(7) Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor CellsCancersMarch 27, 2020Peris-Torres C, Plaza-Calonge MDC, López-Domínguez R, Domínguez-García S, Barrientos-Durán A, Carmona-Sáez P, Rodríguez-Manzaneque JC.proteins. Then, membranes were blocked with 5% low-fat milk and incubated with sheep anti-human ADAMTS1 (AF5867, R&D Systems, Minneapolis, MN, USA) and monoclonal mouse anti-Actin (sc-8432, Santa Cruz Biotechnology,

(8) ADAMTS-1 in abdominal aortic aneurysmPloS oneJanuary 1, 2017Vorkapic E, Folkesson M, Magnell K, Bohlooly-Y M, Länne T, Wågsäter D.solution. Sections were then incubated with human primary antibodies against ADAMTS-1 (2 μg/ ml, Cat. No. AF5867, R&D Systems, Inc, Minneapolis, USA), CD68 (0.1 μg/ml, Cat. No. NCL-CD68-KP1, Leica Microsystems, Newcastle,

(9) A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivoScience signalingOctober 25, 2016Wong JC, Krueger KC, Costa MJ, Aggarwal A, Du H, McLaughlin TL, Feldman BJ.

(10) Stroma-derived but not tumor ADAMTS1 is a main driver of tumor growth and metastasisOncotargetJune 7, 2016Fernández-Rodríguez R, Rodríguez-Baena FJ, Martino-Echarri E, Peris-Torres C, Del Carmen Plaza-Calonge M, Rodríguez-Manzaneque JC.blocked with 5 % low-fat milk and incubated with the monoclonal mouse anti-human ADAMTS1 antibody (AF5867, R&D Systems). After incubation with the appropriate secondary peroxidase-conjugated antibody, signal

(11) Galnt1 is required for normal heart valve development and cardiac functionPloS oneJanuary 1, 2015Tian E, Stevens SR, Guan Y, Springer DA, Anderson SA, Starost MF, Patel V, Ten Hagen KG, Tabak LA.MF-20 (Developmental Studies Hybridoma Bank #MF20; mouse monoclonal; 1:100); ADAMTS1 (R&D Systems #AF5867; sheep polyclonal; 1:100); ADAMTS5 (Thermo Scientific #PA5–27165; rabbit polyclonal; 1:100); ADAMTS20

(12) ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptorExperimental cell researchMay 1, 2014Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek MZ, Obika M, Ohtsuki T, Hofmann M, Kusachi S, et al.

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ADAMTS1 Antibody

ADAMTS1 Antibody

ADAMTS1 Antibody

ADAMTS1 Antibody

ADAMTS1 Antibody

Human/Mouse ADAMTS1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (12)

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Human/Mouse ADAMTS1 Antibody from R&D Systems, a Bio-Techne Brand

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