Fig 2: TNFR2 expression on CD8+ T cells is correlated with canonical markers of exhaustion. A and B, TNFR1 (A) and TNFR2 (B) expression on CD8+ T cells harvested from the tumor, spleen, or tdLNs from tumor-bearing mice over time, across tumor types and locations. N = 10–35 per time point/per tissue. ǂ represents P < 0.0001 between tumor and spleen and between tumor and tdLN. Ɵ represents P < 0.01 between tumor and tdLN and P < 0.0001 between tumor and spleen. C and D, Correlation of TNFR2 expression on tumor-infiltrating CD8+ T cells with expression of canonical markers of exhaustion (PD-1, SLAMF6, TIM3, and TOX) in subcutaneous E0771 (C) and intracranial CT2A (D). Tumors were harvested at similar timepoints as displayed in Fig. 1A and B. A simple linear regression model was performed to determine significance and R2 values. E, Expression of TNFR2 on CD8+ T cells in the spleen of mice infected with chronic LCMV at days 7 and 14 after infection compared with naïve spleen. One-way ANOVA was performed. F–H, Expression of PD-1 (F), TIM3 (F), and TOX (H) on TNFR2− and TNFR2+ CD8+ T cells in the spleen of mice infected with chronic viral infection at peak viral titers (day 14). A paired t test was performed. Data represented as mean ± 95% confidence interval. ns, not significant; *, P < 0.05; **, P < 0.01; ****, P < 0.0001. ic, intracranial; sc, subcutaneous.