Fig 1: Transcriptomic and immunophenotypic characterisation of FAPs.a Principal component analysis of gene expression profiles of FAPs (blue) and SCs (orange) as determined by RNA sequencing, based on the 500 most variably expressed genes (n = 3 for each cell type). b Volcano plot showing differentially expressed genes between FAPs (blue) and SCs (orange), as determined by RNA sequencing (n = 3). Genes corresponding to selected key FAP and SC markers are indicated. Dashed line indicates significance cutoff at a false discovery rate of 0.05. c Box plots showing the expression of selected key FAP (blue) and SC (orange boxes) marker genes (n = 3), as determined by RNA sequencing. Results are expressed in terms of reads per kilobase million (RPKM). d Comparative flow cytometric analysis of FAPs and SCs, based on surface expression of PDGFRα-AF647, ITGA5-APC, CD14-APC, or CD61-APC, each combined with CD56-PE.