Purified anti-mouse CD3 100202 from BioLegend

Product Specs
ItemPurified anti-mouse CD3
CompanyBioLegend
Price $94.00Supplier Page View Company Product Page
Catalog Number100202
NCBI Gene AliasesCtg-3, Ctg3, T3g
ApplicationsFC - Quality tested, IHC-F - Verified, IP, ICC - Reported in the literature, not verified in house
ReactivityMouse
Quantity500 µg
Clone17A2
Conjugate/TagUnconjugated
IsotypeRat IgG2b, kappa
HostRat
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.5mg/ml
ImmunogenγδTCR-positive T-T hybridoma D1
Antibody TypePrimary
ClonalityMonoclonal
TargetCD3

Questions and Answers

Can this anti-mouse CD3 antibody be used for flow cytometry applications?

Yes, the purified anti-mouse CD3 antibody (clone 17A2) has been shown to work effectively in flow cytometry applications, as indicated by its usage in various studies, including those that analyze T cell populations.

What is the recommended dilution for using this antibody in immunohistochemistry?

For immunohistochemistry, the purified anti-mouse CD3 antibody is recommended to be used at a dilution of 1:50 for frozen sections, as noted in the literature.

Has this antibody been utilized in any specific studies related to T cell function or autoimmune diseases?

Yes, this antibody has been referenced in studies investigating T cell activation and function, including research on systemic lupus erythematosus, where it was used to analyze T cell populations and their cytokine production.

What type of immune cells does this antibody specifically target?

The purified anti-mouse CD3 antibody specifically targets CD3, a component of the T cell receptor complex, making it suitable for identifying and studying T cells in various experimental settings.

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) β-Catenin Stabilization Protects Against Alveolar Hemorrhage Through Amphiregulin and BATF-Mediated Regulatory T CellsJCI InsightMarch 23, 2026Fiona Mason, Hui Xiong, Ali Mobeen, Md Saddam Hossain, Sara Mahmudlu, Rosanne Trevail, Mikyal Mobeen, Li Chen, Sunny Lee, Tuncay Delibasi, et al.(130-117-044) from Miltenyi Biotec. Cell were stimulated with precoated plates with purified CD3 (catalog 100202) and CD28 (catalog 122022), both from BioLegend. Cells were stimulated for 5 minutes. For Western blot

(2) Engineered Bacteria‐Vesicle Delivered Lactate Reprogramming Boosts Tumor RadiosensitivityAdvanced ScienceMarch 1, 2026Fei Peng, Zhe Lei, Zhehao Zhang, Zhiyue Su, Chonghai Zhang, Huan Yang, Shu Liu, Mengyuan Hu, Yuhong Wang, Lingchuan Guo, et al.(HY‐17567). For flow cytometry, the following anti‐mouse antibodies were used: CD3‐FITC (Biolegend, 100202), CD4‐APC (Biolegend, B201509), CD8‐PE (Biolegend, 100734), CD11c‐FITC (Elabscience, N418), CD86‐APC

(3) Commensal-myeloid crosstalk in neonatal skin regulates interleukin-1 signaling and cutaneous type 17 inflammationImmunityFebruary 10, 2026Miqdad O. Dhariwala, Ricardo O. Carale, Andrea M. DeRogatis, Henry Rodriguez-Valbuena, Joy N. Okoro, Christina A. Ekstrand, Antonin Weckel, Victoria M. Tran, Irek Habrylo, Rio Barrere-Cain, et al.CD4 (Clone RM4-5),Biolegend,"Cat# 100506, RRID:AB_312709" antimouse CD3 (Clone 17A2),Biolegend,"Cat# 100202, RRID:AB_312659" antimouse B220 (Clone RA3-6B2),Biolegend,"Cat# 103202, RRID:AB_312987" antimouse

(4) IL‐4/STAT6‐signaling Influences Local Inflammation and Regeneration Processes During Acute Pancreatitis and Promotes Fibrosis by a Direct Activation of Pancreatic Fibroblasts During Chronic PancreatitisAdvanced ScienceJanuary 5, 2026ElSheikh ElSheikh, Salvers Salvers, Piesker Piesker, Buchholz Buchholz, Lange Lange, Ameling Ameling, Steil Steil, Hammer Hammer, Homuth Homuth, Völker Völker, et al.700247, Invitrogen), anti‐NFkBp65 (dilution 1:100, #8242, Cell Signaling), anti‐CD3 (dilution 1:100, Cat 100202 Biolegend), anti‐MPO (dilution 1:200, AF3667, R&D systems), anti‐STAT3 (dilution 1:100, D3Z2G, cell

(5) Method for generation and ex vivo expansion of genetically edited mouse TregsJournal of Leukocyte BiologyDecember 1, 2025Yosef Gilad, Adam M Dean, Yan Xia, Sang Jun Han, David M Lonard, Bert W O'Malleyto the manufacturer's protocol. The cells were then plated on an anti-mouse CD3 antibody (BioLegend; 100202) precoated dish in an indicated culture media supplemented with 2 μg/mL anti-mouse CD28 antibody (BioLegend;

(6) Therapeutic radiation drives leptomeningeal dissemination of medulloblastoma through an innate immune processDevelopmental CellNovember 3, 2025Carolina Nör, Kaitlin Kharas, Alex Rasnitsyn, Maria C. Vladoiu, Nam Woo Cho, Jacob S. Young, Felipe Nör, Ncedile Mankahla, Joonas Haapasalo, Kristiina Nordfors, et al.,Becton Dickinson,556999 Ly6B,Serotec,MCA771GA CD68,Abcam,Ab125212 IBA1,Abcam,Ab153496 89Y CD45,Biolegend,103102 111Cd CD3,Biolegend,100202 112Cd CD64,Biolegend,139302 113In Ter119,Biolegend,116202 114Cd CD8,Biolegend,100702 116Cd CD4,Biolegend,100506 139La

(7) Whole-genome sequencing reveals rare and structural variants contributing to psoriasis and identifies CERCAM as a risk geneCell GenomicsOctober 8, 2025Kyuto Sonehara, Rei Watanabe, Yutaka Matsumura, Yuichi Mitsui, Yosuke Ogawa, Kaori Odomari, Saori Sakaue, Shinichi Namba, Mariko Komuro, Mio Edamoto, et al.Antibody (Clone 93),Biolegend,Cat# 101320; RRID:AB_1574975 Purified anti-mouse CD3,Biolegend,"Cat# 100202, RRID:AB_312659" Alexa Flour 488-conjugated anti-rat IgG,Thermo Fisher Scientific,"Cat# A-11006, RRID:AB_2534074" CERCAM Polyclonal antibody

(8) Zeaxanthin augments CD8+ effector T cell function and immunotherapy efficacyCell Reports MedicineSeptember 16, 2025Freya Q. Zhang, Jiacheng Li, Rukang Zhang, Jiayi Tu, Zhicheng Xie, Takemasa Tsuji, Hardik Shah, Matthew O. Ross, Ruitu Lyu, Junko Matsuzaki, et al.Antibody,Biolegend,Cat#302902; Clone#CD28.2;RRID:AB_314304. Purified anti-mouse CD3 Antibody,Biolegend,Cat#100202; Clone#17A2;RRID:AB_312659. Purified anti-mouse CD28 Antibody,Biolegend,Cat#102102; Clone#; 37.51;RRID:AB_312867. Human/mouse/rat

(9) Isolation and Ex Vivo Testing of CD8+ T-Cell Division and Activation Using Mouse SplenocytesBio ProtocAugust 20, 2025Melissa Dolan, Yuhao Shi, Amber McKenery, Stephanie Tzetzo, Kohei Chida, Kazuaki Takabe, Scott I. Abrams, John M.L. EbosAntibody,Purpose,Host,Company,Catalog number Anti-mouse CD3 (clone 17-A2),Cell activation,Rat,BioLegend,100202 Anti-mouse CD28 (clone 37.51),Cell activation,Syrian hamster,BioLegend,102102 Anti-mouse CD16/CD32

(10) Probiotic-mediated tumor microenvironment reprogramming with protease-sensitive interleukin-15 and photothermal therapyCell reports. MedicineJune 17, 2025Wang H, Zheng L, Yang C, Jia L, Zhou R, Liu H, Dong Y, Xu X, Shi G, Yang J, et al.AB_10900082 anti-CD44-AF700,Biolegend,Cat#103026; RRID: AB_493712 anti-CD3,Abcam,Cat#ab5690; RRID: AB_305055 anti-CD3,Biolegend,Cat#100202; RRID: AB_312658 anti-CD8,BD Biosciences,Cat#550281; RRID: AB_2275792 anti-CD49b,BD Biosciences,Cat#553855;

(11) A cowpea mosaic virus adjuvant conjugated to liposomes loaded with tumor cell lysates as an ovarian cancer vaccineNat CommunMay 30, 2025Zhongchao Zhao, Debbie K. Ledezma, Jessica Fernanda Affonso de Oliveira, Anthony O. Omole, Nicole F. Steinmetzantibody (1:100, Biolegend, 103202), and rat anti-mouse CD3 monoclonal antibody (1:100, Biolegend, 100202). The next day, DCs were stained using a goat anti-Armenian hamster TRITC polyclonal antibody (1:500,

(12) PINK1 deficiency rewires early immune responses in a mouse model of Parkinson's disease triggered by intestinal infectionNPJ Parkinsons DisMay 22, 2025Sherilyn Junelle Recinto, Alexandra Kazanova, Lin Liu, Brendan Cordeiro, Shobina Premachandran, Hicham Bessaiah, Alexis Allot, Elia Afanasiev, Sriparna Mukherjee, Jessica Pei, et al.in a 24-well suspension plate pre-coated with 10 μg/mL anti-mouse CD3 antibody (BioLegend, Cat. No. 100202). Cells were then immediately co-stimulated with 5 μg/mL anti-mouse CD28 antibody (BioLegend, Cat. No.

(13) Genetics- and age-driven neuroimmune and disc changes underscore herniation susceptibility and pain-associated behaviors in SM/J miceSci AdvApril 25, 2025Emanuel J. Novais, Olivia K. Ottone, Eric V. Brown, Vedavathi Madhu, Victoria A. Tran, Pranay Ramteke, Abhijit S. Dighe, Michael D. Solga, Alexandra Manchel, Angelo C. Lepore, et al.ab36001), IB4 (1:200, Sigma-Aldrich, L2895), CD19 (1:50, BioLegend, 115569), CD3 (1:50, BioLegend, 100202), Ly6G (1:50, BioLegend, 127602), MHCII (1:50, Thermo Fisher Scientific, 50-112-9377), and F4/80 (1:50,

(14) C3G promotes bone marrow adipocyte expansion and hematopoietic regeneration after myeloablation by enhancing megakaryocyte niche functionJ Hematol OncolApril 1, 2025Óscar Herranz, Pablo Berrocal, Carmen Sicilia-Navarro, Cristina Fernández-Infante, Luis Hernández-Cano, Almudena Porras, Carmen Guerreroadded: anti-Gr-1 (#108401, 1/400), anti-CD11b (#101201, 1/400), anti-CD19 (#115501, 1/200), anti-CD3 (#100201, 1/200), anti-Ter119 (#116201, 1/200). Following incubation, washed Sheep anti-Rat IgG Dynabeads™ (Thermo Fisher Scientific

(15) Severe Acute Respiratory Syndrome Coronavirus 2 Variant Infection Dynamics and Pathogenesis in Transgenic K18-hACE2 and Inbred Immunocompetent C57BL/6J MiceVirusesMarch 30, 2025Hongwei Liu, Brianna M. Ramirez, Talia S. Wong, Christopher M. Weiss, Kevin C. K. Lloyd, Qizhi Gong, Lark L. CoffeyRecognized,Host Species and Format,Source and Catalog Number,RRID,Protocol CD3,Rat monoclonal,BioLegend (100202),AB_312659,(1:400) with fluor-conjugated 2nd antibody CD68,Rat monoclonal,Invitrogen (14-0681-82),AB_2572857,(1:100)

(16) UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemiaLife science allianceMarch 1, 2025Samarakoon Y, Yelland T, Garcia-Gonzalez E, da Silva Justo Junior A, Mahmood M, Manoharan A, Patterson S, Serafin V, Gammage PA, Marmiroli S, et al.used were as follows: CD8 alpha (PerCP-Cy5.5, mouse, Cat #104721; BioLegend), CD3 (APC, mouse, Cat #100202; BioLegend), Va5.1 T-cell receptor (PE, mouse, Cat #139503; BioLegend), CD69 (BV650, mouse, Cat #104541;

(17) Beta cell extracellular vesicle PD-L1 as a novel regulator of CD8+ T cell activity and biomarker during the evolution of type 1 diabetesDiabetologiaFebruary 1, 2025Chaitra Rao, Daniel T. Cater, Saptarshi Roy, Jerry Xu, Andre De G De Oliveira, Carmella Evans-Molina, Jon D. Piganelli, Decio L. Eizirik, Raghavendra G. Mirmira, Emily K. Simsround bottom plates (Greiner Bio One, Cat#650180) were coated with anti-CD3 (0.5 μg/ml, BioLegend Cat# 100202, RRID:AB_312659) and anti-CD28 (1 μg/ml, BD Biosciences Cat# 553294, RRID:AB_394763) antibodies overnight.

(18) Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumorsEMBO reportsJanuary 1, 2025Shi Y, McKenery A, Dolan M, Mastri M, Hill JW, Dommer A, Benzekry S, Long M, Abrams SI, Puzanov I, et al.day, splenocytes were stained with CFSE (Biolegend, 423801), stimulated with mouse αCD3 (Biolegend, 100202) and αCD28 (Biolegend, 102102) according to manufacturer recommendations. To generate conditioned media,

(19) Bispecific antibody targeting of lipid nanoparticlesbioRxivDecember 23, 2024Angelo Amabile, MATTHEW PHELAN, ZHIXIN YU, PEDRO SILVA, Adam Marks, JUDIT MORLA-FOLCH, Moah Sohn, Gurkan Mollaoglu, CHIARA FALCOMATA, Abraham J P Teunissen, et al.experiments,Anti-mouse CD4,Biolegend,Cat#116018 Tethering experiments,Anti-mouse CD3,Biolegend,Cat#100202 Tethering experiments,Anti-mouse CD8b,Biolegend,Cat#126602 Tethering experiments,Anti-mouse CD45,Biolegend,Cat#103101 LNPMal

(20) IRF4 regulates myeloid-derived suppressor cells expansion and function in Schistosoma japonicum-infected miceParasites & VectorsNovember 28, 2024Lu Zhou, Peibin Lin, Guorong Deng, Lengshan Mo, Cansheng Hong, Zhihan Jiang, Yiqiang Zhu, Yi Zhao, Yanwei Qi, Tengfei Hu, et al.Cat. no. 651009t), their corresponding isotype controls and purified anti-mouse CD3 (17A2, Cat. no. 100202)/CD28 (37.51, Cat. no. 122002) antibody were obtained from BioLegend (San Diego, CA, USA). Fluorescein-conjugated

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Purified anti-mouse CD3 from BioLegend

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Purified anti-mouse CD3 from BioLegend

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