Fig 1: Suppression of miR-4461 by antisense oligonucleotides upregulates FGA expression in hepatocytes. Candidate target genes of miR-4461 were selected based on miRNA-binding prediction databases and predominant expressions in hepatocytes. The regulatory effects of miR-4461 were evaluated in HuH7 and HepG2 cells. Cells were transfected with either an antisense oligonucleotide targeting miR-4461 or a control oligonucleotide. A, B CYP2C8; C, D LBP; E, F FGA. A, C, E Immunoblot analysis of protein expressions. B, D, F) Quantification of immunoblot band intensities, presented as relative expression levels. Data represent the mean ± SD from three independent experiments. *P < 0.05.
Fig 2: miR-4461 suppressed FGA expression in hepatocytes. Effects of miR-4461 on candidate target genes were evaluated in HuH7 and HepG2 cells. Cells were transfected with either a miR-4461 mimic or a non-targeting control (miR-NTC), and protein expression was assessed. A, B CYP2C8; C, D LBP; E, F FGA. A, C, E Immunoblot analysis of protein expression. B, D, F) Quantification of immunoblot band intensities, expressed as relative values normalized to control. Data are presented as mean ± SD from three independent experiments. *P < 0.05.
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