Fig 2: EphA2-/- Neutrophils Have Decreased Capacity to Limit Fungal Proliferation In Vivo(A) Flow cytometry of EphA2 surface expression on wild-type neutrophils. Histograms of 5 × 105 events are shown.(B) Wild-type neutrophils were either uninfected (top panel) or infected for 30 min with GFP-expressing C. albicans (bottom panel) and then stained with anti-mouse EphA2 (mEphA2) antibody (Ab) (red) and DAPI (blue). Scale bar, 10 µm.(C) Immunoblot analysis showing the time course of EphA2 phosphorylation in wild-type neutrophils that had been infected with yeast-phase C. albicans SC5314 for the indicated time points.(D) Oral fungal burden of wild-type and EphA2-/- mice that had been treated with either isotype control or anti-Gr-1 antibody and then orally infected with C. albicans for 2 days. Results are median ± interquartile range of five mice per group from two independent experiments. **p < 0.01 (Mann-Whitney test corrected for multiple comparisons). The y axis is set at the limit of detection.(E) Body weight of EphA2-/- mice that had been treated with either isotype control or anti-Gr-1 antibody and then orally infected with C. albicans for 2 days. Results show median of three mice per group from two independent experiments. *p < 0.05 (Mann-Whitney test).(F) Scheme of neutrophil adoptive transfer of orally infected CD18-/- mice. PMNs, polymorphonuclear neutrophils.(G) Oral fungal burden 2 days post-infection of immunocompetent wild-type and CD18-/- mice after adoptive transfer of wild-type or EphA2-/- neutrophils. Results are median ± interquartile range of six mice per group from two independent experiments. **p < 0.01, ***p < 0.001 (Mann-Whitney test corrected for multiple comparisons). The y axis is set at the limit of detection (20 CFUs per gram of tissue).(H) Neutrophil counts after 1 day of infection in the tongues of CD18-/- mice receiving wild-type or EphA2-/- neutrophils. Results are median ± interquartile in a single experiment (n = 4–5). NS, not significant (Mann-Whitney test).See also Figure S3.