Fig 1: High-dose finasteride shows the antioxidant potential in glioblastoma cells. (A) U373 (upper) and T98G cells were subjected to CellROX staining after treatment with vehicle or finasteride (FIN) at the indicated concentration for 24 h. Nuclear DAPI signal is shown in blue. The relative CellROX signal intensities are shown (right). Each dot means an individual cell. (B) Immunoblot analysis of SESN2, SOD2, and PRDX5 in U373 (left) or T98G (right) cells after treatment with vehicle or FIN at the indicated concentration for 24 h. GAPDH was used as a loading control. The relative band intensities of SESN2, SOD2, and PRDX5 are shown below. The intensities of vehicle-treated samples were arbitrarily set to 1. (C) U373 (upper) and T98G cells were subjected to TMRE staining after treatment with vehicle or finasteride (FIN) at the indicated concentration for 24 h. Nuclear DAPI signal is shown in blue. The relative TMRE signal intensities are shown (right). Each dot means an individual cell. * p < 0.05, ** p < 0.01, *** p < 0.001.