Western Blot: DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric [NBP2-52649] - Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 (NBP2-52649) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. NBP2-52649 successfully detected the canonical human DC-SIGNR, as well as multiple other isoforms.
DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric
Supplier Page from Novus Biologicals, a Bio-Techne Brand for DC-SIGNR/CD299/CLEC4M Antibody (16E7) - Chimeric - Azide and BSA Free
Available conjugates: Available conjugates: UnconjugatedSpecificity: Human CD299, which is an oligomeric type II transmembrane protein with a C-type lectin extracellular domain, the expression of which is restricted to immature DC, macrophages in the lung, and endothelial cells in the liver. It binds ICAM-3 and ICAM-7 to mediate the interaction of DC with T lymphocytes and endothelial cells in the initial stages of immune response and in the migratory behavior of DC. CD299 also binds the gp120 protein of HIV and the E2 envelope protein of HCV, thereby playing a role in viral infection.Sizes Available: 0.2 mg (also 0.025 mg)