Flow Cytometry: CD30/TNFRSF8 Antibody (Ki-4) - Chimeric [NBP2-52683] - Flow-cytometry using anti-CD30 antibody Ki-4. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Ki-4 (NBP2-52683) before (B) or after anti-CD3/28 activation (C) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.