Flow Cytometry: MDR1/ABCB1 Antibody (MRK16) - Chimeric [NBP2-52654] - Flow-cytometry using the MDR1/ABCB1 Antibody (MRK16) [NBP2-52654]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (NBP2-52654, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
MDR1/ABCB1 Antibody (MRK16) - Chimeric
Supplier Page from Novus Biologicals, a Bio-Techne Brand for MDR1/ABCB1 Antibody (MRK16) - Chimeric - Azide and BSA Free
Available conjugates: Available conjugates: UnconjugatedSpecificity: Recognizes a discontinuous extracellular epitope on P-glycoprotein.Sizes Available: 0.2 mg (also 0.025 mg)