TRPC6 antibody orb344463 from Biorbyt

Product Specs
ItemTRPC6 antibody
CompanyBiorbyt
Price $292.00Supplier Page
Catalog Numberorb344463
Quantity25 µl
ApplicationsELISA, IHC, WB
Concentration1.0 mg/mL
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityThis product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined
ReactivityHuman, Mouse
TargetTRPC6
IsotypeIgG1
Clone3F2.H10.F2
HostMouse
ImmunogenThis monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein
NCBI Full Gene Nametransient receptor potential cation channel subfamily C member 6
NCBI Gene AliasesFSGS2, TRP6
ClonalityMonoclonal
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing

Specifications/Features

Application Notes: Anti-TRPC6 monoclonal antibody has been tested by ELISA, immunohistochemistry and western blotting. Expect a band approximately 30 kDa in size corresponding to the cytoplasmic domain of TRPC6 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 µg/mL.

Application Dilutions: ELISA: 1:10,000 - 1:50,000, IHC: 2.5 µg/mL, WB: 1:500- 1:2,000

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