HIF3 alpha antibody orb345422 from Biorbyt

Product Specs
ItemHIF3 alpha antibody
CompanyBiorbyt
Price $784.00Supplier Page
Catalog Numberorb345422
Quantity100 µg
ApplicationsELISA, WB
Concentration1.2 mg/mL
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityThis affinity-purified antibody is directed against mouse Hif3alpha protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Hif3a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known
ReactivityMouse
TargetHIF3 alpha
IsotypeIgG
HostRabbit
ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor)
NCBI Full Gene Namehypoxia inducible factor 3, alpha subunit
NCBI Gene AliasesbHLHe17, MOP7, NEPAS, Ipas
ClonalityPolyclonal
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 alpha protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 alpha due to sequence homology.

Application Dilutions: ELISA: 1:14,000 - 1:80,000, WB: 1:1,000 - 1:8,000

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