DNMT1 (D63A6) XP ® Rabbit mAb 5032S from Cell Signaling Technology

Product Specs
ItemDNMT1 (D63A6) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number5032S
Quantity100 µl
ApplicationsW, IHC-P, IF-IC, FC-FP, ChIP
ReactivityH, M, R, Mk
TargetDNMT1
HostRabbit
NCBI Gene AliasesADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI
ClonalityMonoclonal
NCBI Full Gene NameDNA methyltransferase 1
Research AreaChromatin Regulation / Nuclear Function
IsotypeIgG
CloneD63A6
Molecular Weight200

Questions and Answers

What applications has the DNMT1 (D63A6) XP® Rabbit mAb been used for in scientific research?

The DNMT1 (D63A6) XP® Rabbit mAb has been shown to work in various applications including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, and chromatin immunoprecipitation.

Can this antibody be used to study the effects of DNMT1 in cancer research?

Yes, the DNMT1 antibody has been utilized in studies investigating its role in cancer. For instance, it was used in research demonstrating that pharmacological inhibition of DNMT1 can restore macrophage autophagy and alter macrophage polarization in nonalcoholic fatty liver disease, which is relevant to cancer progression (Pant et al., 2023).

Is the DNMT1 antibody effective for detecting DNMT1 in different species?

The DNMT1 (D63A6) XP® Rabbit mAb is reactive with multiple species, including humans, mice, rats, and non-human primates, making it versatile for comparative studies across different model organisms.

How has the DNMT1 antibody been utilized in epigenetic studies?

The DNMT1 antibody has been employed in studies examining the epigenetic regulation of gene expression. For example, it was used to assess the impact of DNMT1 on the methylation status of genes involved in cancer, highlighting its role in maintaining genomic methylation patterns (Chen et al., 2023; Ngan et al., 2023).

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(1) Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy modelEur J HistochemApril 20, 2026Juan Fan, Yu Ma, Liying Sun, Suhua Miao, Jianghong He(C#2972; Cell Signaling Technology, Inc.), p-AKT (#9271; Cell Signaling Technology, Inc.), DNMT1 (#5032; Cell Signaling Technology, Inc.), GAPDH (ab8245; Abcam). HRP-con- jugated secondary antibodies (bs-0295G/0296G;

(2) ACRC/GCNA is an essential protease that repairs DNA-protein crosslinks during vertebrate developmentNucleic Acids ResApril 20, 2026Cecile Otten, Marin Kutnjak, Christine Supina-Pavic, Marija Pranjic, Ivan Anticevic, Vanna Medved, Marta Popovicimmunostained with the following primary antibodies respectively: anti-DNMT1 (Cell Signaling Technology, #5032, 1:1000), anti-TOP2A (Abcam, ab52934, 1:1000), anti-TOP1 (Santa Cruz, sc-271285, 1:1000), anti-PARP1

(3) WNT inhibition activates interferon stimulated gene expression by alleviating epigenetic repression of endogenous retrovirusesScientific ReportsMarch 5, 2026Courtney M. Williams, Jessica Harper Calderon, Varenka Rodriguez DiBlasi, Louis Jinrui Liu, Samer Nuwayhid, Hannah Cevasco, Wei Wang, Rekha Soni, Christina Adler, David D’Ambrosio, et al.phospho-IRF-3 (37829), MDA-5 (5321), RIG-I (3743), P-STING (19781),STING (13647), LSD1 (2184), DNMT1 (5032), EZH2 (5246), ERK (4695), phospho-ERK (4370 ) were purchased from Cell Signaling Technologies and used

(4) DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312Nature CommunicationsFebruary 26, 2026Ying Fu, Yu Xiang, Yun Han, Juan Cai, Shaobin Duan, Anqun Chen, Zheng DongAF1817), GAPDH (Proteintech, 10494), β-actin (Proteintech, 66009), DNMT1 (Cell Signaling Technology, 5032), DNMT3a (abcam, ab188470), DNMT3b (abcam, ab2851) and FLAG (Sigma-Aldrich, 102698217), as appropriate.

(5) Regulation of BCL11A DNA binding and expression in human erythrocyte precursor HUDEP-2 cellsbioRxivFebruary 12, 2026Meigen Yu, Puspa Das, John R. Horton, Jujun Zhou, Jisun Lee, Tingting Hong, Yue Lu, Marcos R. Estecio, Polina A. Iakova, Abhinav K. Jain, et al.primary antibodies overnight at 4°C. Primary antibodies included DNMT1 (Cell Signaling Technology - CST #5032), H3K9me2 (CST #4658), H3K27me3 (CST #9733s), H3 (CST #14269), EED (CST #51673), GAPDH (CST #5174),

(6) Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progressionCell Death & DiseaseJanuary 30, 2026Shizhou Yang, Yuejiang Ma, Tingting Wu, Xiufeng Huangs,1:2000,WB ERK,CST,4695 s,1:1000,WB p-MEK,CST,9154 T,1:1000,WB MEK,proteintech,11049-1-AP,1:20000,WB DNMT1,CST,5032 T,1:1000/1:100,WB/ IF CD68,proteintech,25747-1-AP,1:5000,IF CD206,proteintech,18704-1-AP,1:500,IF Ki67,Abclonal,A20018,1:500,IHC "Anti-rabbit

(7) Loss of MicroRNA-29b promotes DNMT3b-mediated STING downregulation to attenuate radiotherapy-induced antitumor immunity in KRAS-mutated colorectal cancernpj Precision OncologyJanuary 28, 2026Hsin-Yu Chang, Jhen-Yu Chen, Tao-Wei Ke, Yi-Wen Jiang, Wei-Ze Hong, Yuan-Yao Tsai, Shu-Fen Chiang, Yu-Sen Lin, Chi-Hsien Huang, Te-Hong Chen, et al.iReal Biotech.), calreticulin (#12238, Cell Signaling Technology), beta-actin (Ab8227, Abcam), DNMT1 (#5032, Cell Signaling Technology), DNMT3a (#49768, Cell Signaling Technology), DNMT3b (#57868, Cell Signaling Technology

(8) Reprogramming the epigenetic profile improves the B regulatory cell function of patients with recurrent pregnancy lossCell Communication and SignalingJanuary 23, 2026Fei Ma, Qing Xu, Lingzhi Xu, Yuanyi Zhang, Xiaoyang Feng, Yanyu Ye, Ping Tang, Pingchang Yang, Yan Ninginhibitor).Antibodies: Anti-TRIM28 (Abcam, ab10483; validated for ChIP/Co-IP), anti-DNMT1 (Cell Signaling, 5032; ChIP-validated), anti-K48-linked ubiquitin (Millipore, 05–1307; validated for ubiquitination assays),

(9) TRIM37-PARP1-TET1 axis maintains stemness and prevents osteoporosis by inhibiting DNMT1 alternative splicing via 5hmC regulationNat CommunDecember 16, 2025Chun-Te Ho, Ling-Hui Li, Wei-Chao Chang, Heng-Hsiung Wu, Ya-Huey Chen, Shih-Chieh Hung(GTX627420, GeneTex), TET2 (ab94580, Abcam, Cambridge, UK), PARP1 (#9532, Cell Signaling Technology), DNMT1 (#5032, Cell Signaling Technology, Danvers, MA), pSer1981-ATM (GTX132146, Gene Tex), TRIM37 (GTX114565, GeneTex),

(10) Ring Finger Protein 1, a Novel Ubiquitin E3 Ligase Targeting Cancerous Inhibitor of Protein Phosphatase 2A to Suppress Smoking-Induced Lung TumorigenesisMedComm (2020)December 7, 2025In-Ho Jeong, Jae Kwang Yun, Jun‐O. Jin, Geun Dong Lee, Peter Chang-Whan Lee(#2368, Cell Signaling Technologies), anti‐E2F1 (#SC‐56661, Santa Cruz Biotechnology), anti‐DNMT1 (#5032, Cell Signaling Technologies). Sequences of siRNAs, shRNAs, and primers are described in the Table S1. Cycloheximide

(11) Discovery of a Novel DNMT1 Inhibitor with Improved Efficacy in Treating β‐ThalassemiaAdvanced ScienceDecember 5, 2025Yijie Shen, Jiale Wei, Shibing Tang, Dongliang Wu, Liangyi Zong, Shuyuan Ma, Qing Xiong, Ruijie Gong, Siyuan Xu, Chuxuan Peng, et al.Antibodies Western blotting was performed using standard procedures. Primary antibodies: DNMT1 (1:1000, CST, Cat. #5032), DNMT3A (1:1000, Proteintech, Cat. #20954‐1‐AP), DNMT3B (1:1000, Proteintech, Cat. #26971‐1‐AP), HBG1/2

(12) Study on Abnormal Angiogenesis in Moyamoya Disease via Mitochondrial D‐Loop MethylationBrain and BehaviorNovember 22, 2025Yuting Luo, Heng Ye, Chutong Guo, Shaoqing Wu, Xunsha Sun5% milk for 1 h. Antibodies were diluted with the use of a primary antibody diluent: DNMT: 1:1:1000 (5032T, Cell Signaling Technology, USA); GAPDH: 1:3000 (Cell Signaling Technology, USA); GAPDH: 1:1:1000; the

(13) MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progressionOncogeneOctober 7, 2025Hiroki Ozawa, Yin Wang, Henry G. Withers, Naoki Haratake, Ayako Nakashoji, Atrayee Bhattacharya, Atsushi Fushimi, Chie Kikutake, Kazuhiro Yamanoi, Shaowen White, et al.Santa Cruz, Dallas, TX, USA and ab316860, 1:1000 dilution; abcam, Cambridge, MA, USA), anti-DNMT1 (5032, 1:1000 dilution; CST), anti-DNMT3A (3598, 1:1000 dilution; CST), anti-DNMT3B (67259, 1:1000 dilution;

(14) Knockdown of ACC1 promotes migration and invasion of U251 glioma cells by epigenetically suppressing SDHInternational journal of oncologySeptember 1, 2025Wei X, Wang Y, Zhao W, Yang W, Tang J, Zhao B, Liu Y.no. 4190), cyclin D1 (1:1,000; cat. no. 2922), p21 (1:1,000; cat. no. 2947), DNMT1 (1:1,000; cat. no. 5032), histone H3 acetylation at lysine 9 (H3K9ac; 1:1,000; cat. no. 9649) and H3 (1:1,000; cat. no. 4499)

(15) Osteopontin derived from hypoxia-induced M2 macrophages promotes osteosarcoma progression through modulation of EGR3/ISG15 signaling and RIG-I expressionJ Transl MedAugust 21, 2025Chunyang Xing, Wei Hu, Liyuan ZhaoName,Brand,Number β-actin,CST,4970 HIF1A,Abcam,ab179483 E-cadherin,Proteintech,"20,874-1-AP" N-cadherin,Abclonal,A19083 Vimentin,Abclonal,A19607 p-STAT3,CST,9145S STAT3,Proteintech,"10,253-2-AP" p-AKT,CST,4060s AKT,Proteintech,"60,203-2-lg" p-P13K,CST,4228 P13K,Proteintech,"60,225-1-Ig" p-ERK,CST,p-ERK ERK,Proteintech,"16,443-1-AP" p-NFκB,CST,3033S NFκB,CST,#8242 OPN,Abcam,ab69498 EGR3,Abcam,ab232820 RIG-I,Santa Cruz,sc-376845 PD-L1,CST,13684T ISG15,Sigma,HPA004627 DNMT1,CST,5032 Anti-mouse IgG,CST,7076P2 Anti-rabbit IgG,CST,7074P2 HRP-conjugated anti-mouse IgG,Servicebio,G1214 HRP-conjugated

(16) Deletion of EP3 prostaglandin receptor in murine macrophages aggravates diet-induced obesity by suppressing SPARCThe EMBO JournalJuly 23, 2025Wenlong Shang, Yinxiu Li, Lu Wang, Jiao Liu, Huiwen Ren, Qian Liu, Shumin Guo, Yuhong Wang, Yubo Ma, Tianyi You, et al.blocking solution) overnight at 4 °C. The primary antibodies were diluted as follows: rabbit anti-Dnmt1 (#5032, 1:1000; Cell Signaling Technology, Boston, MA, USA), rabbit anti-Dnmt3a (#ab188470, 1:2000; Abcam,

(17) Allosteric activation of the SPRTN protease by ubiquitin maintains genome stabilityNature communicationsJuly 21, 2025Dürauer S, Kang HS, Wiebeler C, Machida Y, Schnapka DS, Yaneva D, Renz C, Götz MJ, Weickert P, Major AC, et al.Thermo Scientific) using MOPS buffer and subsequently immunoblotted using anti-DNMT1 antibody (1:1000) (#5032, Cell Signaling), anti-SPRTN antibody (1:500) (6F2) (ref. 26), anti-RNF4 antibody (1:500) (AF7964, R&D systems

(18) DNMT1 blocks SOX21-repressed CKS2 transcription to promote gastric cancer progressionBMC CancerJuly 17, 2025Jie Wei, Song Xue, Xinglong Du, Yuanfei Dai, Yuting Ji, Guangsi Heab9485) were purchased from Abcam (Cambridge, UK), and the primary antibodies against DNMT1 (1:1000, 5032), E-Cadherin (1:1000, 3195), N-Cadherin (1:1000, 4061), and Vimentin (1:1000, 5741) were purchased from

(19) Exposing the DNA methylation-responsive compartment of the leukaemic genome in T-ALL cell lines support its potential as a novel therapeutic target in T-ALLClin EpigeneticsJuly 3, 2025Maike Bensberg, Aida Selimović-Pašić, Lisa Haglund, Júlia Goldmann, Sandra Hellberg, Colm E. Nestormembranes were incubated with primary antibodies against DNMT1 (1:1000, Cell Signalling Technology, 5032, RRID:AB_10548197), Phospho-Histone H2A.X (1:1000, Cell Signalling Technology, 9718) or β-actin (1:1000,

(20) Low expression of SGCA promotes lung squamous cell carcinoma malignant progressionSci RepJuly 2, 2025Guojie Chen, Guangyao Mao, Xinyu Zong, Xiao Chen, Guo Ting, Mei LinMMP2 (Cat. No.40994; Cell Signaling, USA), MMP9 (Cat. No.13667; Cell Signaling, USA), Bax (Cat. No.5032; Cell Signaling, USA) and Bcl-2 (Cat. No.4423; Cell Signaling, USA). Next, the membranes were further

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DNMT1 (D63A6) XP ® Rabbit mAb

DNMT1 (D63A6) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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DNMT1 (D63A6) XP ® Rabbit mAb from Cell Signaling Technology

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