Smad2 antibody orb345610 from Biorbyt

Product Specs
ItemSmad2 antibody
CompanyBiorbyt
Price $292.00Supplier Page
Catalog Numberorb345610
Quantity25 µl
ApplicationsELISA, IP, WB
Concentration0.55 mg/mL
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
PurityThis affinity purified antibody is directed against human Smad2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. Also, the antibody is Smad2 specific, and reactivity to other Smad proteins (specifically Smad1, Smad3, Smad4, and Smad7) is not detected in over-expressed cell lysates (Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD)
ReactivityHuman, Monkey
TargetSmad2
IsotypeIgG
HostRabbit
ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein
NCBI Full Gene NameSMAD family member 2
NCBI Gene AliasesJV18, MADH2, LDS6, hMAD-2, hSMAD2, MADR2, JV18-1, CHTD8
ClonalityPolyclonal
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing

Specifications/Features

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract.

Application Dilutions: ELISA: 1:100,000, IP: User Optimized, WB: 1:1,000 - 1:3,000

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