Immunofluorescence analysis of shown to co-localize with recombinant protein using PBK1 antibody|Western blot analysis of HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate using PBK1 antibody
Supplier Page from Biorbyt for PBK1 antibody
Application Notes: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PBK1 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous PBK1. For immunofluorescence microscopy, fix cells with methanol.
Application Dilutions: ELISA: 1:2,500 - 1:10,000, IF: 1:100, IP: 1:100, WB: 1:500 - 1:2,000