AKT1 PE Antibody orb344551 from Biorbyt

Product Specs
ItemAKT1 PE Antibody
CompanyBiorbyt
Price $920.00Supplier Page
Catalog Numberorb344551
Quantity50 ug
ApplicationsDOT, FC
Concentration1.0 mg/mL
Conjugate/TagRPE
FormatLyophilized
PurityAnti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected
ReactivityHuman, Mouse, Rat
TargetAKT1 PE
IsotypeIgG2a
Clone14E5.A2.B2.H9
HostMouse
ImmunogenAnti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development
NCBI Full Gene NameAKT serine/threonine kinase 1
NCBI Gene AliasesRAC, RAC-ALPHA, PKB-ALPHA, PRKBA, AKT, PKB
ClonalityMonoclonal
StorageStore vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis

Specifications/Features

Application Notes: Anti-AKT1 PE Antibody is tested for Flow Cytometry. This antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Application Dilutions: FC: 1.0 µg/mL

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