S100 Protein antibody orb344628 from Biorbyt

Product Specs
ItemS100 Protein antibody
CompanyBiorbyt
Price $970.00Supplier Page
Catalog Numberorb344628
Quantity500 ug
ApplicationsELISA, IHC, WB
Conjugate/TagUnconjugated
PurityThis Protein A purified antibody is directed against bovine S100 protein. The product was purified from monospecific antiserum by Protein A chromatography. A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar
TargetS100 Protein
IsotypeIgG
ImmunogenThis Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers)
NCBI Full Gene NameS100 calcium binding protein A1
ClonalityPolyclonal
HostRabbit
Concentration5.0 mg/mL
ReactivityBovine, Human, Mouse, Rat
FormatLyophilized
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Notes: This Protein A purified antibody has been tested for use in immunohistochemistry, and western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 11 kDa in size corresponding to S100 monomer by western blotting in the appropriate cell lysate or extract.

Application Dilutions: ELISA: 1:5,000 - 1:25,000, IHC: 1:200 - 1:2,000, WB: 1:500 - 1:3,000

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